[논문]추출용매에 따른 톳(Hizikia fusiformis) 추출물의 항산화 및 생리활성 비교

이연지; 전유진; 김용태

doi:10.5657/kfas.2020.0886

추출용매에 따른 톳(Hizikia fusiformis) 추출물의 항산화 및 생리활성 비교
Comparison of Antioxidant and Physiological Activities of Various Solvent Extracts from Hizikia fusiformis 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.53 no.6, 2020년, pp.886 - 893

이연지 (군산대학교 식품생명공학전공) , 전유진 (제주대학교 수산생명의학전공) , 김용태 (군산대학교 식품생명공학전공)

Abstract ▼ AI-Helper

The seaweed Hizikia fusiformis is rich in protein, carbohydrates, vitamins, and minerals. This study investigated the antioxidant and physiological activities of H. fusiformis extracts prepared with 70% ethanol, 80% methanol, or distilled water. The extraction yields of these various solvent extracts were as follows: ethanol extract, 15.26%; methanol extract, 17.95%; and water extract, 45.62%. The methanol extract showed the highest total polyphenol content (24.06 mg GAE/g), but total flavonoid content was similar in all extracts. ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging activity was highest in the ethanol extract (IC50: 0.90 mg/mL), while the methanol extract exhibited the strongest DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity (IC50: 8.09 mg/mL), reducing power (EC50: 0.40 mg/mL), and ferric reducing antioxidant power (0.28 mM). By contrast, tyrosinase and α-glucosidase inhibitory activities were higher in the ethanol extract than in the other extracts. The high BACE1 (β-secretase) inhibitory activity was observed in the ethanol extract (IC50: 1.03 mg/mL). These results indicate that H. fusiformis ethanol extracts may be useful for their antioxidant and functional properties in food and pharmaceutical materials.

주제어

표/그림 (6)

표 Table 1. Comparison of the extraction yields and color values of various solvent extracts from Hizikia fusiformis
그림 Fig 1. ABTS (A) and DPPH radical scavenging activities (B) of various solvent extracts from Hizikia fusiformis. ABTS, 2,2'-azino- bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt; DPPH, 2,2-diphenyl-1-picrylhydrazyl.
표 Table 3. Antioxidant activities of various solvent extracts from Hizikia fusiformis
표 Table 2. Total polyphenol and total flavonoid contents of various solvent extracts from Hizikia fusiformis
표 Table 4. Tyrosinase, α-glucosidase and BACE1 inhibitory activities of various solvent extracts (5 mg/mL) from Hizikia fusiformis
그림 Fig 2. BACE1 (β-secretase) inhibitory activities of various solvent extracts from Hizikia fusiformis.

참고문헌 (37)

Ali MY, Kim DH, Seong SH, Kim HR, Jung HA and Choi JS. 2017. α-Glucosidase and protein tyrosine phosphatase 1B inhibitory activity of plastoquinones from marine brown alga Sargassum serratifolium. Mar Drugs 15, 368. https://doi.org/10.3390/md15120368.

상세보기
Benzie IFF and Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 239, 70-76. https://doi.org/10.1006/abio.1996.0292.

상세보기
Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1200. https://doi.org/10.1038/1811199a0.

상세보기
Byun HG and Kim SK. 2005. Development of physiological activity and health functional materials of seaweed.Susanyeon-gu 22, 1-10.
Cho EK and Choi, YJ. 2010. Physiological activities of hot water extracts from Ecklonia cava kjellman. J Life Sci 20, 1675-1682. https://doi.org/10.5352/JLS.2010.20.11.1675.

원문보기 상세보기
Choi BW, Lee BH, Kang KJ, Lee ES and Lee NH. 1998. Screening of the tyrosinase inhibitors from marine algae and medicinal plants. Korean J Pharmacogn 29, 237-242.
Choi JH, Kim JS, Jo BS, Kin J, Park HJ, An BJ, Kim M, Cho YJ. 2011. Biological activity in functional cosmetic purple sweet potato extracts. Korean J Food Preserv 18, 414-422. https://doi.org/10.11002/kjfp.2011.18.3.414.

원문보기 상세보기
Choi MW, Jung CG, Kim HR and Kim JI. 2017. Effect of Sargassum serratifolium extracts on β-amyloid production. Korean J Fish Aquat Sci 50, 85-91. https://doi.org/10.5657/KFAS.2017.0085.

원문보기 상세보기
Cui YR, Kim HS, Je JG, Wang L, Oh JY, Jia L and Jeon YJ. 2019. Protective effects of antioxidant active fractions derived from the edible seaweed Hizikia fusiformis in oxidatively stressed human dermal fibroblasts. Korean J Fish Aquat Sci 52, 35-42. https://doi.org/10.5657/KFAS.2019.0035.

원문보기 상세보기
Iida K, Hase K, Shimomura K, Sudo S, Kadota S and Namba T. 1995. Potent inhibitors of tyrosinase activity and melanin biosynthesis from Rheum officinale. Planta Med 61, 425-428. http://dx.doi.org/10.1055/s-2006-958129.

상세보기
Jeon MJ, Kim M, Jang HJ, Lee SW, Kim JH, Kim HS and Lee SH. 2012. Whitening effect of Hizikia fusiformis ethanol extract and its fractions. J Life Sci 22, 889-896. https://doi.org/10.5352/JLS.2012.22.7.889.

원문보기 상세보기
Kim JH, Kang HM, Lee SH, Lee JY and Park LY. 2015. Antioxidant and α-glucosidase inhibition activity of seaweed extracts. Korean J Food Preserv 22, 290-296. https://doi.org/10.11002/kjfp.2015.22.2.290.

원문보기 상세보기
Kim MJ, Lee HH, Seo MJ, Kang BW, Park JU and Jeong YK. 2013. Antioxidation activities of organic solvent fractions obtained from seaweed, Hizikia fusiformis. J Life Sci 23, 361-367. https://doi.org/10.5352/JLS.2013.23.3.361.

원문보기 상세보기
Kim PH, Kim MJ, Kim JH, Lee JS, Kim KH, Kim HJ, Jeon YJ, Heu MS and Kim JS. 2014. Nutritional and physiologically active characterizations of the sea squirt Halocynthia roretzi sikhae and the seasoned sea squirt. Korean J Fish Aquat Sci 47, 1-11. https://doi.org/10.5657/KFAS.2014.0001.

원문보기 상세보기
Kim SH, Lim SB, Ko YH, Oh CK, Oh MC and Park CS. 1994. Extraction yields of Hizikia fusiforme by solvents and their antimicrobial effects. Korean J Fish Aquat Sic 27, 462-468.
Kwon MS, Mun OJ, Bae MJ, Lee SG, Kim MH, Lee SH, Yu KH, Kim YY and Kong CS. 2015. Anti-inflammatory activity of ethanol extracts from Hizikia fusiformis fermented with lactic acid bacteria in LPS-stimulated RAW264.7 macrophages. J Korean Soc Food Sci Nutr 44, 1450-1457. https://doi.org/10.3746/jkfn.2015.44.10.1450.

원문보기 상세보기
Kwon YR and Youn KS. 2015. Quality characteristics of Hijikia fusiforme extracts with different extraction method. Korean J Food Preserv 22, 70-77. https://doi.org/10.11002/kjfp.2015.22.1.70.

원문보기 상세보기
Kwon YR and Youn KS. 2017. Antioxidant and physiological activities of Hijikia fusiforme by extraction methods. Korean J Food Preserv 24, 631-637. https://doi.org/10.11002/kjfp.2017.24.5.631.

원문보기 상세보기
Lee KW, Kim YS, Park PJ, Jeong JH. 2014. Comparison of effect of water and ethanolic extract from roots and leaves of Allium hookeri. J Korean Soc Food Sci Nutr 43, 1808-1816. https://doi.org/10.3746/jkfn.2014.43.12.1808.

원문보기 상세보기
Lee NY. 2013. Antioxidant effect and tyrosinase inhibition activity of seaweeds ethanol extracts. J Korean Soc Food Sci Nutr 42, 1893-1898. https://doi.org/10.3746/jkfn.2013.42.12.1893.

원문보기 상세보기
Lee SH. 2011. Current status and prospect of nutraceuticals from marine algae. Bull Food Technol 24, 165-175.
Lee YJ, Kim WS, Jeon YJ and Kim YT. 2020a. Physicochemical properties and antioxidant activities of the yanggaeng containing Hizikia fusiformis powder. Korean J Fish Aquat Sci 53, 588-596. https://doi.org/10.5657/KFAS.2020.0588.

원문보기 상세보기
Lee YJ, Kim WS, Jeon YJ and Kim YT. 2020b. Physicochemical properties and antioxidant activities of the yanggaeng containing Pyropia yezoensis. Korean J Fish Aquat Sci 53, 672-680. https://doi.org/10.5657/KFAS.2020.0672.

원문보기 상세보기
Lee YJ, Kim WS, Lee BJ, Jeon YJ and Kim YT. 2017. Quality characteristics and antioxidant activities of gruel Containing Saccharina japonica powder. Korean J Fish Aquat Sci 50, 707-713. https://doi.org/10.5657/KFAS.2017.0707.

원문보기 상세보기
Lee YJ, Lim SY, Kim WS and Kim YT. 2016. Processing and quality characteristics of glutinous barley gruel containing Hizikia fusiformis. Korean J Fish Aquat Sci 49, 310-316. https://doi.org/10.5657/KFAS.2016.0310.

원문보기 상세보기
Lee YJ. 2017. Expression, purification and characterization of BACE1 (β-secretase) in E. coli. M.S. Thesis, Kunsan National Univeristy, Gunsan, Korea.
Middleton E and Kandaswami C. 1994. Potential health-promoting properties of citrus flavonoids. Food Technol 48, 115-119.
Moreno MI, Isla MI, Sampietro AR and Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71, 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0.

상세보기
Oyaizu N, Yasumizu R, Miyama-Inaba M, Nomura S, Yoshida H, Miyawaki S, Shibata Y, Mitsuoka S, Yasunaga K and Morii S. 1988. (NZW×BXSB) F1 mouse. A new animal model of idiopathic thrombocytopenic purpura. J Exp Med 167, 2017-2022. https://doi.org/10.1084/jem.167.6.2017

상세보기
Park SJ, Tai S, Lee YJ and Kim YT. 2014. Overexpression and refolding of BACE2. Korean J Fish Aquat Sci 47, 370-375. https://doi.org/10.5657/KFAS.2014.0370.

원문보기 상세보기
Re R, Pellegrini N, Proteggente A, Pannala A, Yang Min and Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Bio Med 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3.

상세보기
Shetty K, Curtis OF, Levin RE, Witkowsky R and Ang V. 1995. Prevention of vitrification aßociated with in vitro shoot culture of oregano. (Origanum vulgare) by Pseudomonas spp. J Plant Physiol 147, 447-451. https://doi.org/10.1016/S0176-1617(11)82181-4.

상세보기
Siriwardhana N, Lee KW, Kim SH, Ha JW and Jeon YJ. 2003. Antioxidant activity of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Sci Tech Int 5, 339-346. https://doi.org/10.1177/1082013203039014.

상세보기
Son HJ, Um MY, Kim I, Cho S, Han D and Lee C. 2016. In vitro screening for anti-dementia activities of seaweed extracts. J Korean Soc Food Sci Nutr 45, 966-972. https://doi.org/10.3746/jkfn.2016.45.7.966.

원문보기 상세보기
Statistics Korea. 2020. Fishery production survey. Retrieved from http://kosis.kr on Jun 18, 2020.
Wagle A, Seong SH, Zhao BT, Woo MH, Jung HA and Choi JS. 2018. Comparative study of selective in vitro and in silico BACE1 inhibitory potential of glycyrrhizin together with its metabolites, 18α- and 18β-glycyrrhetinic acid, isolated from Hizikia fusiformis. Arch Pharm Res 41, 409-418. https://doi.org/10.1007/s12272-018-1018-2.

상세보기
Watanabe J, Kawabata J, Kurihara H and Niki R. 1997. Isolation and identification of α-glucosidase inhibitors from Tochucha (Eucommia ulmoides). Biosci Biotech Biochem 61, 177-178. https://doi.org/10.1271/bbb.61.177.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증