Lee, Yongcheol
(Seoul Metropolitan Government Research Institute of Public Health and Environment)
,
Lee, Jea-Kyoo
(Seoul Metropolitan Government Research Institute of Public Health and Environment)
,
Kim, Jeong-Gon
(Seoul Metropolitan Government Research Institute of Public Health and Environment)
,
Park, So-Hyun
(Seoul Metropolitan Government Research Institute of Public Health and Environment)
,
Kim, Young-Eun
(Seoul Metropolitan Government Research Institute of Public Health and Environment)
,
Park, Sung-Kyu
(Seoul Metropolitan Government Research Institute of Public Health and Environment)
,
Kim, Moo-Sang
(Seoul Metropolitan Government Research Institute of Public Health and Environment)
Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities we...
Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities were investigated for comparison of the biological activity of the berry extracts. Chlorogenic acid was the major phenolic acid, which had the highest content in aronia, followed by blueberry. The rutin and isoquercetin contents were the highest in mulberry, followed by aronia, black raspberry, and blueberry. The anthocyanin content was the highest in black raspberry. Aronia showed the highest DPPH free radical and nitrite scavenging activities with the highest contents of total phenolics and proanthocyanidins.
Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities were investigated for comparison of the biological activity of the berry extracts. Chlorogenic acid was the major phenolic acid, which had the highest content in aronia, followed by blueberry. The rutin and isoquercetin contents were the highest in mulberry, followed by aronia, black raspberry, and blueberry. The anthocyanin content was the highest in black raspberry. Aronia showed the highest DPPH free radical and nitrite scavenging activities with the highest contents of total phenolics and proanthocyanidins.
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제안 방법
All experiments were performed in triplicate and data were expressed as means±standard deviations. To compare differences among the data, statistical analysis was performed using the SPSS program (SPSS version 20.
05. Correlation analysis was performed among total phenolics, anthocyanins, proanthocyanidins, DPPH, and nitrite scavenging activity. The strength of the correlation relationship was determined by Pearson correlation coefficient r and p-level.
The mobile phase consisted of deionized water with 2% (v/v) acetic acid (solvent A) and methanol (solvent B). The flow rate was 0.3mL/min with the following gradient elution program: 0-2min, 5% B; 2-7min linear gradient from 5 to 20% B; 7-10min 20% B; 10-13min linear gradient from 20 to 30% B; 13-15min 30% B; 15-18min linear gradient from 30 to 40% B; 18-20min 40% B; 20-22min linear gradient from 40 to 50% B; 22-24min 50% B; 24-28min linear gradient from 50 to 95% B; 28-30min 95% B; 30-31min linear gradient from 95 to 5% B; and 4min reconditioning. The injection volume was 2µL and detector wavelengths were 254, 280, and 360 nm (scan range: 200-600nm).
The identification and quantification of nonanthocyanin phenolics were carried out by HPLC system (Agilent 1290 Infinity Series, Agilent Technologies) coupled with a 6495 triple quadrupole mass spectrometer (Agilent Technologies) equipped with an electrospray interface. The separation was conducted on an Atlantis T3 column (3µm, 2.
0) and the data analyzed by analysis of variance (ANOVA). After the ANOVA analysis, significant differences were determined with Duncan’s multiple range test at p<0.05. Correlation analysis was performed among total phenolics, anthocyanins, proanthocyanidins, DPPH, and nitrite scavenging activity.
Correlation analysis was performed among total phenolics, anthocyanins, proanthocyanidins, DPPH, and nitrite scavenging activity. The strength of the correlation relationship was determined by Pearson correlation coefficient r and p-level.
as means±standard deviations. To compare differences among the data, statistical analysis was performed using the SPSS program (SPSS version 20.0) and the data analyzed by analysis of variance (ANOVA). After the ANOVA analysis, significant differences were determined with Duncan’s multiple range test at p<0.
이론/모형
The nitrite scavenging activity was determined using the method of Kato et al. [17] and Kang et al.
The total anthocyanin content was measured using the AOAC pH differential method [14]. The extracts were diluted separately in volumetric flask with pH 1.
The total phenolics content was determined by the Folin-Ciocalteu method [13]. A total of 0.
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