Lee, Sulhee
(Research Group of Healthcare, Korea Food Research Institute)
,
Cho, Eui-Sang
(Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University)
,
Nam, Young-Do
(Research Group of Healthcare, Korea Food Research Institute)
,
Park, So-Lim
(Research Group of Healthcare, Korea Food Research Institute)
,
Lim, Seong-Il
(Research Group of Healthcare, Korea Food Research Institute)
,
Seo, Dong-Ho
(Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University)
,
Kim, Jae-Hwan
(Advanced Geo-materials Research Department, Pohang Branch, Korea Institute of Geoscience and Mineral Resources)
,
Seo, Myung-Ji
(Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University)
A total of 262 bacterial strains were isolated from clay minerals, bentonite and zeolite, in Gyeongsangbukdo, Republic of Korea, and their hydrolytic enzyme activities were analyzed. Most of the isolated strains belonged to Micrococcales and Bacillales order. Of strains, 96 strains produced α...
A total of 262 bacterial strains were isolated from clay minerals, bentonite and zeolite, in Gyeongsangbukdo, Republic of Korea, and their hydrolytic enzyme activities were analyzed. Most of the isolated strains belonged to Micrococcales and Bacillales order. Of strains, 96 strains produced α-amylase activity, 42 strains showed cellulase activity, 111 strains had pectinase activity, and 70 strains showed protease activity. Among them, 177 isolates exhibited one or more of the hydrolytic enzyme activities and in particular Bacillus cereus MBLB1321, B. albus MBLB1326 and KIGAM017, B. mobilis MBLB1328, MBLB1329 and MBLB1330 showed all of the enzyme activities. These results demonstrate the diversity of functional Bacillus species in clay minerals as vital sources for the discovery of industrially valuable hydrolytic enzymes, which have a great commercial prospect in various bio-industrial applications.
A total of 262 bacterial strains were isolated from clay minerals, bentonite and zeolite, in Gyeongsangbukdo, Republic of Korea, and their hydrolytic enzyme activities were analyzed. Most of the isolated strains belonged to Micrococcales and Bacillales order. Of strains, 96 strains produced α-amylase activity, 42 strains showed cellulase activity, 111 strains had pectinase activity, and 70 strains showed protease activity. Among them, 177 isolates exhibited one or more of the hydrolytic enzyme activities and in particular Bacillus cereus MBLB1321, B. albus MBLB1326 and KIGAM017, B. mobilis MBLB1328, MBLB1329 and MBLB1330 showed all of the enzyme activities. These results demonstrate the diversity of functional Bacillus species in clay minerals as vital sources for the discovery of industrially valuable hydrolytic enzymes, which have a great commercial prospect in various bio-industrial applications.
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제안 방법
Although there have been lots of studies on physiological characteristics and biological activities of clay minerals themselves [13], there are not much data published on the isolation of microorganisms from clay minerals and evaluation of their physicochemical activities. In this study, we isolated microorganisms from domestic clay minerals, zeolites and bentonite to investigate cultivable microbial diversity and also evaluated their hydrolytic enzyme activities such as cellulase, pectinase, amylase, and protease. In addition, we finally identified clay minerals-derived microorganisms with multi-hydrolytic enzyme activity to secure the microbial sources from domestic clay minerals and consider their applications into bio-industries.
Universal bacterial primer pairs 27F (5'-AGAGTTTGATCMTGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') were used to amplify 16S rRNA gene for the bacterial identification. The amplified 16S rRNA genes were sequenced by Macrogensequencing service (Macrogen Inc., Korea) and their sequences then subjected to a homology search against the NCBI database (BLAST) and EzBioCloud (http://www.ezbiocloud.net/eztaxon/) [14]. After the identification of 262 isolated strains, Pseudarthrobacter sp.
대상 데이터
One bentonite and four zeolite samples were respectively collected at the Youngil, Gampo and Daebo mining areas located in Gyeongsangbuk-do, Republic of Korea(Table 1). Clay mineral samples were homogenized with distilled water to measure pH and NaCl concentration(%, w/v) by pH meter (Fisher Science Education, USA)and HI 96821 Refractometer (HANNA instruments, USA), respectively.
이론/모형
Neighbor-joining (NJ) phylogenetic tree based on 16S rRNA gene sequences showing the position and relationship of strains isolated from domestic bentonite and zeolite samples and other related taxa. The evolutionary distances were calculated using the Kimura two-parameter model. Numbers at nodes indicate bootstrap values (>70%) calculated based on the NJ.
성능/효과
In conclusion, the diverse members of Micrococcales and Bacillales order were isolated from clay minerals,zeolite and bentonite, which were collected at domestic mining areas in Gyeongsangbuk-do, Republic of Korea. Total 262 isolates were also evaluated to determine the hydrolytic enzyme production including α-amylase, cellulase, pectinase, and protease.
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