[논문]루틴, 퀘르세틴, 식이섬유 혼합물 설계를 이용한 전분소화 지연 효과의 최적화에 대한 융합 연구

오임경; 배인영

doi:10.15207/jkcs.2020.11.5.035

루틴, 퀘르세틴, 식이섬유 혼합물 설계를 이용한 전분소화 지연 효과의 최적화에 대한 융합 연구
Convergence Study on the Optimization for Suppression of Starch Hydrolysis using Rutin, Quercetin and Dietary Fiber Mixture Design 원문보기

한국융합학회논문지 = Journal of the Korea Convergence Society, v.11 no.5, 2020년, pp.35 - 41

오임경 (국립순천대학교 식품공학과) , 배인영 (극동대학교 식품영양학과)

초록
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본 연구는 루틴, 퀘르세틴, 식이섬유가 전분 소화율에 미치는 영향을 통계적 혼합물 설계방법을 이용하여 확인하고자 하였다. 세 가지 성분들은 모두 농도가 증가함에 따라 전분소화 지연 효과가 나타났으며, 그 중에서도 퀘르세틴과 식이섬유가 함께 섞였을 때 가장 높은 지연 효과를 보였다. 이 혼합물 설계는 simplex-centroid 실험설계법을 이용하였고, 최적 모델은 quadratic 모델에서 나타났다. 이때 얻어진 회귀방정식을 통하여 유의한 상승효과를 확인할 수 있었다. 최적화 통계 방법을 사용하여 혼합비를 분석한 결과 퀘르세틴과 식이섬유가 72: 28 비율에서 전분 소화 지연 효과가 최대로 나타나는 것을 확인하였다. 전분소화에 대한 영향을 3차원 표면 도표로 시각화하여 나타내었으며, 이 결과로 퀘르세틴과 식이섬유의 상호작용으로 인하여 전분 소화를 15% 이상 지연시키는 것을 확인할 수 있었다.

Abstract ▼ AI-Helper

This study was conducted to develop the efficient system for starch hydrolysis suppression using rutin, quercetin and dietary fiber through the statistical mixture design. The three components were replaced with wheat flour at the level of 10% and the mixed gel with three components was characterized by in vitro starch digestion. The mixture design was applied by simplex-centroid experimental model. The quadratic model (R2=0.86) was well fitted and the obtained regression equation indicated that the significant positive effects was observed in the quercetin and fiber mixture. Based on the statistical results, the best mixing ratio of quercetin and fiber was 72: 28 that led to the lowest predicted glycemic index (pGI). Their interactions on the pGI of starch digestibility were clearly visualized in the 3D surface plot. These results suggested that the mixture of quercetin and fiber interact strongly with wheat flour, consequently retarding starch hydrolysis by 15%.

주제어

표/그림 (4)

표 Table 1. Mixture design for 10 trials with three components and their responses
표 Table 2. List of summary statistics of the fitted models for the response of predicted glycemic index
그림 Fig. 1. Schematic plots of (a) ternary contour and (b) 3D surface plots for the response of predicted glycemic index of the fitted quadratic mixture model
표 Table 3. Analysis of variance (ANOVA) of the fitted quadratic mixture model for the response of predicted glycemic index

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문제 정의

The beneficial effect of dietary fiber is usually attributed to delays starch degradation in the accordance with physical mechanisms including restriction of water and entrapping starch granule [14]. Based on these properties, the synergistic effect of quercetin and dietary fiber in vitro starch digestibility could be explained in this study. Indeed, further study on physico- chemical charcteristics of their interaction is needed to provided to explain accurately this effect.
The objective of this work was to investigate the combined influences of rutin, quercetin and dietary fiber in vitro starch digestibility. That is, three components were substituted with wheat flour at the 5 levels that designed by statistical method and then their effects in vitro starch digestibility were analyzed.

제안 방법

The objective of this work was to investigate the combined influences of rutin, quercetin and dietary fiber in vitro starch digestibility. That is, three components were substituted with wheat flour at the 5 levels that designed by statistical method and then their effects in vitro starch digestibility were analyzed. The quercetin and fiber mixture was the most effective in retarding starch hydrolysis and their ratio was obtained 72:28 by optimization tool.
The design of experiment is a statistical method to investigate the interaction of the rutin, quercetin and dietary fiber in vitro starch digestibility and also to determine their optimal levels. Table 1 shows the results of the experimental data using simplex-centroid design.
The experimental measurements were conducted in triplicate. The data were analyzed by one-way analysis of variance, and the means were compared by Duncan’s multiple range test at p<0.
The mixture design of the experiment was used to obtain the optimal mixture composition of three components, which were included dietary fiber, rutin and quercetin and selected based on literature research as independent variables, for retarding the starch hydrolysis in vitro starch digestion model system. The experimental design of mixed flour gels was performed using a Design expert software (DX10, Stat-Ease, Inc.
Therefore, the purpose of this study was to investigate the combined effects and optimal levels of the three components (rutin, quercetin and dietary fiber) that delay the starch digestibility by using a statistical method using simplex-centroid design.

대상 데이터

, China). Salivary amylase (A0521, EC 3.2.1.1), pancreatin from porcine pancreas (P7545), bile extract (B8631), and amyloglucosidase from Aspergillus niger (A9931, EC 3.2.1.3) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A total starch assay kit (K-TSTA) and a glucose oxidase-peroxidase assay kit (K-GLUC) were obtained from Megazyme International Ireland Ltd.
The dietary fiber (95% purity), rutin (95% purity), and quercetin (88% purity) from Tartary buckwheat (Fagopyrum tartaricum) were used in this study (Hunan Huacheng Biotech, Inc., China). Salivary amylase (A0521, EC 3.

데이터처리

The data were analyzed by one-way analysis of variance, and the means were compared by Duncan’s multiple range test at p<0.05 by using a SPSS software (version 25, SPSS Inc., Chicago, IL, USA).

성능/효과

As can be seen in the Fig. 1(a), the effects of all individual components and binary interaction of fiber/quercetin had positive effects, while the binary interactions of fiber/rutin and rutin/quercetin synergistically gave negative effects on the pGI in vitro starch digestibility.
The quercetin and fiber mixture was the most effective in retarding starch hydrolysis and their ratio was obtained 72:28 by optimization tool. The various statistical models were analyzed to find valid experimental model and the regression equation which was represented synergic effect on the suppression of starch hydrolysis was obtained. Although more study is needed to investigate the interaction with quercetin and dietary fiber, its combination may be useful for lowering starch digestibility.

참고문헌 (17)

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표제어: PCR

동의어: Packet Collision Rate

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