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Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-ᴅ-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus 원문보기

Journal of microbiology and biotechnology, v.30 no.6, 2020년, pp.946 - 954  

Ju, Jung-Hun (Department of Bioscience and Biotechnology, Konkuk University) ,  Kang, Su-Hwan (Department of Bioscience and Biotechnology, Konkuk University) ,  Kim, Tae-Hun (Department of Bioscience and Biotechnology, Konkuk University) ,  Shin, Kyung-Chul (Department of Bioscience and Biotechnology, Konkuk University) ,  Oh, Deok-Kun (Department of Bioscience and Biotechnology, Konkuk University)

Abstract AI-Helper 아이콘AI-Helper

Platycodon grandiflorum root (Platycodi radix) saponins, platycosides, have been used as health supplements and food items for the treatment of respiratory disorders and pulmonary diseases. Deglycosylated saponins have been known to exert stronger biological effects than their glycosylated forms. In...

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  • This is the first report on the quantitative enzymatic production of 3-O-β-D-glucopyranosyl platycosides and the establishment of the biotransformation pathways.
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참고문헌 (21)

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  2. 2 Chung JW Noh EJ Zhao HL Sim JS Ha YW Shin EM 2008 Anti-inflammatory activity of prosapogenin methyl ester of platycodin D via nuclear factor-kappaB pathway inhibition Biol. Pharm. Bull. 31 2114 2120 10.1248/bpb.31.2114 18981583 

  3. 3 Jang KJ Kim HK Han MH Oh YN Yoon HM Chung YH 2013 Anti-inflammatory effects of saponins derived from the roots of Platycodon grandiflorus in lipopolysaccharidestimulated BV2 microglial cells Int. J. Mol Med. 31 1357 1366 10.3892/ijmm.2013.1330 23563392 

  4. 4 Halliwell B 2006 Oxidative stress and neurodegeneration: where are we now? J. Neurochem. 97 1634 1658 10.1111/j.1471-4159.2006.03907.x 16805774 

  5. 5 Park S Yoo J Kim H Choi Y Choi H Song J 2013 The physiochemical characteristics and anti-oxidant activity of extracts of Platycodon grandiflorus (Jacquin) A. De Candolle Planta Med. 79 1267 1268 10.1055/s-0033-1352423 

  6. 6 Choi YH Kim YS Yeo SJ Roh SH Jeong YC Kang JS 2008 Ameliorating effect of balloon flower saponin on the ethanolinduced memory impairment in mice Phytother. Res. 22 973 976 10.1002/ptr.2394 18521966 

  7. 7 Yoo KY Park OK Hwang IK Li H Ryu SY Kang IJ 2008 Induction of cell proliferation and neuroblasts in the subgranular zone of the dentate gyrus by aqueous extract from Platycodon grandiflorum in middle-aged mice Neurosci. Lett. 444 97 101 10.1016/j.neulet.2008.07.057 18675316 

  8. 8 Chun J Joo EJ Kang M Kim YS 2013 Platycodin D induces anoikis and caspase-mediated apoptosis via p38 MAPK in AGS human gastric cancer cells J. Cell Biochem. 114 456 470 10.1002/jcb.24386 22961809 

  9. 9 Park CS Yoo MH Noh KH Oh DK 2010 Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases Appl. Microbiol. Biotechnol. 87 9 19 10.1007/s00253-010-2567-6 20376631 

  10. 10 Shin KC Oh DK 2016 Classification of glycosidases that hydrolyze the specific positions and types of sugar moieties in ginsenosides Crit. Rev. Biotechnol. 36 1036 1049 10.3109/07388551.2015.1083942 26383974 

  11. 11 Li W Zhao LC Wang Z Zheng YN Liang J Wang H 2012 Response surface methodology to optimize enzymatic preparation of deapio-platycodin D and platycodin D from radix platycodi Int. J. Mol. Sci. 13 4089 4100 10.3390/ijms13044089 22605967 

  12. 12 Jeong EK Ha IJ Kim YS Na YC 2014 Glycosylated platycosides: Identification by enzymatic hydrolysis and structural determination by LC-MS/MS J. Sep. Sci. 37 61 68 10.1002/jssc.201300918 24327461 

  13. 13 Ha IJ Kang M Na YC Park Y Kim YS 2011 Preparative separation of minor saponins from platycodi radix by high-speed countercurrent chromatography J. Sep. Sci. 34 2559 2565 10.1002/jssc.201100326 21812112 

  14. 14 Ahn HJ You HJ Park MS Johnston TV Ku S Ji GE 2018 Biocatalysis of platycoside E and platycodin D 3 using fungal extracellular beta-glucosidase responsible for rapid platycodin D Production Int. J. Mol. Sci. 19 2671 10.3390/ijms19092671 30205574 

  15. 15 Wie HJ Zhao HL Chang JH Kim YS Hwang IK Ji GE 2007 Enzymatic modification of saponins from Platycodon grandiflorum with Aspergillus niger J. Agric. Food Chem. 55 8908 8913 10.1021/jf0716937 17927135 

  16. 16 Kang SH Kim TH Shin KC Ko YJ Oh DK 2019 Biotransformation of food-derived saponins, platycosides, into deglucosylated saponins including deglucosylated platycodin D and their anti-inflammatory activities J. Agr. Food Chem. 67 1470 1477 10.1021/acs.jafc.8b06399 30652865 

  17. 17 Tang ZY Hou YY Hu XY Liu AN Yau LF Tong TT 2017 Metabolite identification and pharmacokinetic study of platycodi radix (Jiegeng) in vivo Rsc. Adv. 7 37459 37466 10.1039/C7RA04814A 

  18. 18 Son JW Kim HJ Oh DK 2008 Ginsenoside Rd production from the major ginsenoside Rb(1) by beta-glucosidase from Thermus caldophilus Biotechnol. Lett. 30 713 716 10.1007/s10529-007-9590-4 17989924 

  19. 19 Kil TG Kang SH Kim TH Shin KC Oh DK 2019 Enzymatic biotransformation of balloon flower root saponins into bioactive platycodin D by deglucosylation with Caldicellulosiruptor bescii beta-glucosidase Int. J. Mol. Sci. 20 3854 10.3390/ijms20163854 31394870 

  20. 20 Nyakudya E Jeong JH Lee NK Jeong YS 2014 Platycosides from the Roots of Platycodon grandiflorum and their health benefits Prev. Nutr. Food Sci. 19 59 68 10.3746/pnf.2014.19.2.059 25054103 

  21. 21 Ryu CS Kim CH Lee SY Lee KS Choung KJ Song GY 2012 Evaluation of the total oxidant scavenging capacity of saponins isolated from Platycodon grandiflorum Food Chem. 132 333 337 10.1016/j.foodchem.2011.10.086 26434298 

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