[논문]Chemical Composition and Antimicrobial Activity of Essential Oil Extracted from Eucalyptus citriodora Leaf

Insuan, Wimonrut; Chahomchuen, Thippayarat

doi:10.4014/mbl.1912.12016

Chemical Composition and Antimicrobial Activity of Essential Oil Extracted from Eucalyptus citriodora Leaf 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.48 no.2, 2020년, pp.148 - 157

Insuan, Wimonrut (Department of Veterinary Technology, Kasetsart University) , Chahomchuen, Thippayarat (Department of Veterinary Technology, Kasetsart University)

Abstract ▼ AI-Helper

Eucalyptus oil is a rich source of bioactive compounds with a variety of biological activities and is widely used in traditional medicine. Eucalyptus citriodora is cultivated for the production of essential oils. However, the mode of antibacterial action of essential oils from E. citriodora is not well-known. This study aimed to determine the chemical components, microbial inhibitory effect, and mechanism of action of the essential oil from E. citriodora. The oil was extracted from E. citriodora leaves by hydro-distillation and the chemical components were analyzed using gas chromatography-mass spectrometry. The antibacterial activities of eucalyptus oil against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, and Staphylococcus intermedius) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) were screened by disc diffusion method and quantitative analysis was conducted by the microdilution method. The mechanism of action of the extracted essential oil was observed using SEM and analyzed by SDS-PAGE. The major components of E. citriodora oil were citronellal (60.55 ± 0.07%), followed by dl-isopulegol (10.57 ± 0.02%) and citronellol (9.04 ± 0.03%). The antibacterial screening indicated that E. citriodora oil exhibited prominent activity against all tested strains. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against B. subtilis were 0.5% and 1.0%, respectively. The MIC and MBC concentrations against S. aureus, S. intermedius, E. coli, and P. aeruginosa were 1% and 2%, respectively. As observed by SEM, the antibacterial mechanism of E. citriodora oil involved cell wall damage; SDS-PAGE revealed decrease in protein bands compared to untreated bacteria. Thus, E. citriodora oil showed significant antimicrobial properties and caused cellular damage.

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문제 정의

Therefore, the objectives of present study were to investigate the chemical compositions of essential oil extracted from E. citriodora cultivated in northern Thailand, and to evaluate the antibacterial properties and the possible mode of action against S. intermedius and P. aeruginosa through changes in their bacterial microstructure and bacterial whole-cell proteins. At the same time, additional information on antibacterial activity against three common pathogenic bacteria including, Bacillus subtilis, Staphylococcus aureus and Escherichia coli were also investigated.

제안 방법

Based on the results of the disc diffusion assay, the oil showed an antibacterial effect against all tested bacteria. E. citriodora oil was selected for further observation of the antibacterial activity using the broth dilution method for quantitative analysis.
The secondary metabolites such as phenolic compound that is found in essential oil with potent anti-microbial activities [14]. The antimicrobial activities of E. citriodora oil against various microorganisms and their activity were qualitatively and quantitatively analyzed using inhibition zone diameters and MIC and MBC values. The MIC and MBC values of essential oil extracted from E.
The experiment was carried out in triplicate and the results were expressed as mean ± standard deviations.

대상 데이터

Fresh leaves were collected from a E. citriodora plantation of a company in Lampang province, northern Thailand at 18°22'12.3''N 99°32'16.5''E during September 2018.

이론/모형

aeruginosa was investigated. The antibacterial mechanism of action was observed by investigating the morphological alterations using SEM analysis. The SEM results revealed that essential oil induced potential cellular damage which the morphology of P.
The antimicrobial activity of Eucalyptus oil was determined using the agar disc diffusion method. Briefly, the cell suspension of microorganisms in MH broth was adjusted to 0.

성능/효과

0 mg/ml [13]. In agreement with our results, the essential oil extracted from E. citriodora leaves cultivated in Thailand had great potential antibacterial activities against all tested strains both gram-positive (B. subtilis, S. aureus and S. intermedius) and gram-negative (E. coli and P. aeruginosa) with MIC and MBC values in the range 0.5 to 1.0% (v/v). The MBC/MIC ratio of E.
citriodora oil showed that the protein bands were smeared and had almost disappeared in all incubation periods, especially the bands with low molecular weights less than 25 kDa. The SDSPAGE results showed the whole protein patterns of both S. intermedius and P. aeruginosa being of low density or to have disappeared.
The antibacterial mechanism of action was observed by investigating the morphological alterations using SEM analysis. The SEM results revealed that essential oil induced potential cellular damage which the morphology of P. aeruginosa seem to be much more severely damage than S. intermedius with time dependent. These supported the possible mechanism being E.
The results of the tested strains after treatment with E. citriodora oil showed that the protein bands were smeared and had almost disappeared in all incubation periods, especially the bands with low molecular weights less than 25 kDa.

참고문헌 (35)

Hirsch EB, Tam VH. 2010. Impact of multidrug-resistant Pseudomonas aeruginosa infection on patient outcomes. Expert Rev. Pharmacoecon. Outcomes Res. 10: 441-451.

상세보기
Haenni M, Hocquet D, Ponsin C, Cholley P, Guyeux C, Madec JY, et al. 2015. Population structure and antimicrobial susceptibility of Pseudomonas aeruginosa from animal infections in France. BMC Vet. Res. 11: 9.

상세보기
Fernandes MR, Sellera FP, Moura Q, Carvalho MPN, Rosato PN, Cerdeira L, et al. 2018. Zooanthroponotic transmission of drug-resistant Pseudomonas aeruginosa, Brazil. Emerg. Infect. Dis. 24: 1160-1162.

상세보기
Bannoehr J, Guardabassi L. 2012. Staphylococcus pseudintermedius in the dog: taxonomy, diagnostics, ecology, epidemiology and pathogenicity. Vet. Dermatol. 23: 253-266.

상세보기
Lainhart W, Yarbrough ML, Burnham CA. 2018. The Brief Case: Staphylococcus intermedius group-look what the dog dragged In. J. Clin. Microbiol. 56: PMID: 29367308 .

상세보기
O'Bryan CA, Pendleton SJ, Crandall PG, Ricke SC. 2015. Potential of plant essential oils and their components in animal agriculture - in vitro studies on antibacterial mode of action. Front. Vet. Sci. 2: 35.

상세보기
Swamy MK, Akhtar MS, Sinniah UR. 2016. Antimicrobial properties of plant essential oils against human pathogens and their mode of action: An updated review. Evid-Based Complement. Alternat. Med. 2016: 3012462.

상세보기
Siramon P, Ohtani Y, Ichiura H. 2013. Chemical composition and antifungal property of eucalyptus camaldulensis leaf oils from Thailand. Rec. Nat. Prod. 7: 49-53.
Elaissi A, Rouis Z, Salem NA, Mabrouk S, ben Salem Y, Salah KB, et al. 2012. Chemical composition of 8 eucalyptus species' essential oils and the evaluation of their antibacterial, antifungal and antiviral activities. BMC Complement. Altern. Med. 12: 81.

상세보기
Bardaweel S, Hudaib M, Tawaha K. 2014. Evaluation of antibacterial, antifungal, and anticancer activities of essential oils from six species of Eucalyptus. J. Essent. Oil Bear Plants 17: 1165-1174.
Barbosa LC, Filomeno CA, Teixeira RR. 2016. Chemical variability and biological activities of Eucalyptus spp. essential oils. Molecules 21: 12.
Salem MZM, Elansary HO, Ali HM, El-Settawy AA, Elshikh MS, Abdel-Salam EM, et al. 2018. Bioactivity of essential oils extracted from Cupressus macrocarpa branchlets and Corymbia citriodora leaves grown in Egypt. BMC Complement Altern. Med. 18: 23.

상세보기
Mulyaningsih S, Sporer F, Reichling J, Wink M. 2011. Antibacterial activity of essential oils from Eucalyptus and of selected components against multidrug-resistant bacterial pathogens. Pharm. Biol. 49: 893-899.

상세보기
Tolba H, Moghrani H, Benelmouffok A, Kellou D, Maachi R. 2015. Essential oil of Algerian Eucalyptus citriodora: Chemical composition, antifungal activity. J. Mycol. Med. 25: e128-e133.

상세보기
Aldoghaim FS, Flematti GR, Hammer KA. 2018. Antimicrobial activity of several cineole-rich western australian eucalyptus essential oils. Microorganisms 6: PMID: 30513933.

상세보기
Wayne P. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard. pp. 15-48. Clinical and laboratory Standards Institute.
Agizzio AP, Da Cunha M, Carvalho AO, Oliveira MA, Ribeiro SF, Gomes VM. 2006. The antifungal properties of a 2S albumin-homologous protein from passion fruit seeds involve plasma membrane permeabilization and ultrastructural alterations in yeast cells. Plant Sci. 171: 515-522.

상세보기
Haddad M, Zein S, Shahrour H, Krivoruchko E, Chokr A, Kanaan H. 2017. Eucalyptus oil prevents Staphylococcus epidermidis bioilm formation. EJPMR 4: 35-41.
Costa AV, Pinheiro PF, de Queiroz VT, Rondelli VM, Marins AK, Valbon WR, et al. 2015. Chemical composition of essential oil from Eucalyptus citriodora leaves and insecticidal activity against Myzus persicae and Frankliniella schultzei. J. Essent. Oil Bear Plants 18: 374-381.

상세보기
Almas I, Innocent E, Machumi F, Kisinza W. 2019. Effect of geographical location on yield and chemical composition of essential oils from three Eucalyptus species growing in Tanzania. Asian J. Tradit. Med. 14: 1-12.
Sultana S, Ali M, Ansari SH, Bagri P. 2008. The effect of physical factors on chemical composition of the essential oil of Eucalyptus citriodora Hook. leaves. J. Essent. Oil Bear Plants 11: 69-74.
Orchard A, van Vuuren S. 2017. Commercial essential oils as potential antimicrobials to treat skin diseases. Evid-Based Complement. Alternat. Med. 4517971: 1-92.
Zhang J, Ye KP, Zhang X, Pan DD, Sun YY, Cao JX. 2016. Antibacterial activity and mechanism of action of black pepper essential oil on meat-borne Escherichia coli. Front. Microbiol. 7: 2094.

상세보기
Li ZH, Cai M, Liu YS, Sun PL, Luo SL. 2019. Antibacterial activity and mechanisms of essential oil from Citrus medica L. var. sarcodactylis. Molecules 24: 8.
Lu H, Shao X, Cao J, Ou C, Pan D. 2016. Antimicrobial activity of eucalyptus essential oil against Pseudomonas in vitro and potential application in refrigerated storage of pork meat. Int. J. Food Sci. Tech. 51: 994-1001.

상세보기
Saleem S, Ahmed B, Khan MS, Al-Shaeri M, Musarrat J. 2017. Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants. Microb. Pathog. 111: 375-387.

상세보기
Chen C, Liu C-H, Cai J, Zhang W, Qi W-L, Wang Z, et al. 2017. Broad-spectrum antimicrobial activity, chemical composition and mechanism of action of garlic (Allium sativum) extracts. Food Control. 86: 117-125.
Zengin H, Baysal AH. 2014. Antibacterial and antioxidant activity of essential oil terpenes against pathogenic and spoilage-forming bacteria and cell structure-activity relationships evaluated by SEM microscopy. Molecules 19: 17773-17798.

상세보기
Winska K, Maczka W, Lyczko J, Grabarczyk M, Czubaszek A, Szumny A. 2019. Essential oils as antimicrobial agents-myth or real alternative? Molecules 24: 1-21.
Lertsatitthanakorn P, Taweechaisupapong S, Arunyanart C, Aromdee C, Khunkitti W. 2010. Effect of citronella oil on time kill profile, leakage and morphological changes of Propionibacterium acnes. J. Essent. Oil Res. 22: 270-274.

상세보기
Fei P, Ali M, Gong S, Sun Q, Bi X, Liu S, et al. 2018. Antimicrobial activity and mechanism of action of olive oil polyphenols extract against Cronobacter sakazakii. Food Control. 94: 289-294.

상세보기
Peng L, Kang S, Yin Z, Jia R, Song X, Li L, et al. 2015. Antibacterial activity and mechanism of berberine against Streptococcus agalactiae. Int. J. Clin. Exp. Pathol. 8: 5217-5223.
Lin L, Chen W, Li C, Cui H. 2019. Enhancing stability of Eucalyptus citriodora essential oil by solid nanoliposomes encapsulation. Ind. Crops Prod. 140: 111615.

상세보기
Salehi B, Sharifi-Rad J, Quispe C, Llaique H, Villalobos M, Smeriglio A, et al. 2019. Insights into Eucalyptus genus chemical constituents, biological activities and health-promoting effects. Trends Food Sci. Tech. 91: 609-624.

상세보기
Aldawsari HM, Badr-Eldin SM, Labib GS, El-Kamel AH. 2015. Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in vivo evaluation. Int. J. Nanomedicine 10: 893-902.

상세보기

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