[논문]Anti-inflammatory effect of Malus domestica cv. Green ball apple peel extract on Raw 264.7 macrophages

Lee, Eun-Ho; Park, Hye-Jin; Kim, Byung-Oh; Choi, Hyong-Woo; Park, Kyeung-Il; Kang, In-Kyu; Cho, Young-Je

doi:10.3839/jabc.2020.016

Anti-inflammatory effect of Malus domestica cv. Green ball apple peel extract on Raw 264.7 macrophages 원문보기

Journal of applied biological chemistry, v.63 no.2, 2020년, pp.117 - 123

Lee, Eun-Ho (School of Food science & Biotechnology, Kyungpook National University) , Park, Hye-Jin (School of Food science & Biotechnology, Kyungpook National University) , Kim, Byung-Oh (School of Food science & Biotechnology, Kyungpook National University) , Choi, Hyong-Woo (Department of Plant Medicals, Andong National University) , Park, Kyeung-Il (Department of Horticulture and Life Science, Yeungnam University) , Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University) , Cho, Young-Je (School of Food science & Biotechnology, Kyungpook National University)

Abstract ▼ AI-Helper

We examined the anti-inflammatory effect of the peel extract of the newly bred Korean apple (Malus domestica Borkh.) cultivar Green ball. To test its possible use as anti-inflammatory functional material, Raw 264.7 macrophages were treated with pro-inflammatory lipopolysaccharide (LPS) in the presence or absence of Green ball apple peel ethanol extract (GBE). Notably, up to 500 ㎍/mL of GBE did not result in any signs of inhibition on cellular metabolic activity or cytotoxicity in Raw 264.7 macrophages. Supplementation with GBE to LPS-treated Raw 264.7 macrophage significantly suppressed various pro-inflammatory responses in a dose-dependent manner, including i) nitric oxide (NO) production, ii) accumulation of inducible NO synthase and cyclooxygenase-2, iii) phosphorylation of nuclear factor-kappa B (NF-κB) subunit p65, and iv) expression of pro-inflammatory biomarker genes, including tumor necrosis factor alpha, interleukin 1 beta, interleukin 6, monocyte chemoattractant protein-1, and prostaglandin E synthase 2.

주제어

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문제 정의

Thus, in this study, we aimed to evaluate the anti-inflammatory function of apple peel extract of the newly bred Korean apple (Malus domestica Borkh.) cultivar Green ball by measuring alteration of lipopolysaccharide (LPS)-induced inflammatory responses in Raw 264.7 macrophage.

제안 방법

For quantification of relative iNOS, COX-2, NF-κB p65, and p-NF-κB p65, densitometry was performed using an imaging system (C300, Azure Biosystems Inc., Dublin, CA, USA).

대상 데이터

This cultivar was developed by Apple Research Institute (National Institute of Horticultural & Herbal Science, Gunwi 39000, Korea) in 2008 by crossing the ‘Golden Delicious’ and ‘Fuji’ varieties. The apple fruit samples were harvested from trees (/M.9, 8 years old) grown at Apple Research Institute, washed with distilled water, and apple peels were collected for further experiments. Collected apple peels were lyophilized in a freeze dryer (FD8518; ilShin BioBase, Yangju, Korea).

데이터처리

One-way analysis of variance (ANOVA) and Duncan’s multiple range test were used to determine the significance of differences between the samples at p <0.05 or p <0.01.

이론/모형

To confirm the cytotoxic effect of GBE, cell viability of Raw 264.7 macrophages was measured using an MTT assay (Fig. 1). Treatment with different concentrations of GBE did not alter cellular metabolic activities.

성능/효과

Consistent with GBE’s suppressive effect on LPS-induced NO production in Raw 264.7 macrophages, iNOS expression was significantly reduced by 99.97, 72.74, 54.96, 41.51, and 31.73% at 100, 200, 300, 400, and 500 μg/mL, respectively (Fig. 3A).

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