[논문]해산 규조류(Skeletonema costatum)의 개체군 성장률 분석을 이용한 신방오도료(Irgarol, Diuron)의 독성평가

이주욱; 최훈; 박윤호; 이승민; 최윤석; 허승; 황운기

doi:10.23005/ksmls.2020.5.1.9

해산 규조류(Skeletonema costatum)의 개체군 성장률 분석을 이용한 신방오도료(Irgarol, Diuron)의 독성평가
Toxic Evaluation of Antifouling Paint (Irgarol and Diuron) using the Population Growth Rate of Marine Diatom, Skeletonema costatum 원문보기

한국해양생명과학회지 = Journal of marine life science, v.5 no.1, 2020년, pp.9 - 16

이주욱 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) , 최훈 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) , 박윤호 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) , 이승민 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) , 최윤석 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) , 허승 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) , 황운기 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터)

초록
AI-Helper

Irgarol과 diuron에 각각 노출된 해산규조류(Skeletonema costatum)의 개체군 성장률(r)을 분석하여 독성평가를 실시하였다. S. costatum을 irgarol (0, 0.31, 0.63, 1.25, 2.5, 5 ㎍ l^-1)과 diuron (0,7.81, 15.63, 31.25, 62.5, 125 ㎍ l^-1)에 각각 96시간 노출하여 r을 계산하였고, 대조구의 r은 0.04 이상으로 시험기준에 적합하였다. S. costatum의 r은 irgarol에서 0.63 ㎍ l^-1, diuron은 15.63 ㎍ l^-1 이상에서 대조구 대비 유의하게 감소하기 시작하여 irgarol과 diuron의 농도가 증가할수록 r이 감소하는 농도의존적인 반응을 나타냈고, 시험 최고농도 5와 125 ㎍ l^-1에서 r이 대조구 대비 80% 이상 감소하였다. Irgarol과 diuron에 노출시킨 S. costatum r의 반수영향농도(EC₅₀)는 1.09, 45.45 ㎍ l^-1, 무영향농도(NOEC)는 0.31, 7.81 ㎍ l^-1, 최소영향농도(LOEC)는 0.63, 15.63 ㎍ l^-1으로 나타났으며, EC₅₀ 기준으로 irgarol이 diuron보다 독성이 큰 것으로 나타났다. 본 연구결과로 해양생태계에서 Irgarol과 diuron의 농도가 0.63과 15.63 ㎍ l^-1 이상이 되면, 해산 규조류 S. costatum의 r은 독성영향을 받을 것으로 판단된다. 또한, 독성 값으로 제시된 결과들은 irgarol과 diuron의 기준농도 설정을 위한 기초자료로 유용하게 활용될 것이다.

Abstract ▼ AI-Helper

We evaluated the toxic effects of antifouling paint (irgarol and diuron) on the population growth rate (r) of the marine diatom, Skeletonema costatum. The r of S. costatum was determined after 96 hrs of exposure to irgarol (0, 0.31, 0.63, 1.25, 2.5 and 5 ㎍ l-1) and diuron (0, 7.81, 15.63, 31.25, 62.5 and 125 ㎍ l-1). It was observed that r in the control (absence of irgarol and diuron) were greater than 0.04, while r in the treatment groups decreased with increasing irgarol and diuron concentrations. Irgarol and diuron reduced r in a dose-dependent manner with significant decreases occurring at concentrations above 0.63 and 15.63 ㎍ l-1, respectively. The EC50 values of r in irgarol and diuron exposure were 1.09 and 45.45 ㎍ l-1. No observed effect concentration (NOEC) were 0.31 and 7.81 ㎍ l-1, the lowest observed effect concentration (LOEC) were 0.63 and 15.63 ㎍ l-1. This result indicate that a concentration of greater than 0.63 ㎍ l-1 of irgarol and 15.63 ㎍ l-1 of diuron in marine ecosystems induced to decreasing r of S. costatum. Also, these toxic values can be useful as a baseline data for the toxic evaluation of irgarol and diuron in marine ecosystems.

주제어

표/그림 (8)

그림 Fig. 1. Cell density of Skeletonema costatum exposed at different concentrations of irgarol.
표 Table 1. Experimental culture condition of Skeletonema costatum
그림 Fig. 2. Change of population growth rate of Skeletonema cos-tatum exposed to irgarol. Vertical bars represent means ± SD for three replicates. ND: not detected. **p<0.01 for control.
그림 Fig. 3. Cell density of Skeletonema costatum exposed at different concentrations of diuron.
그림 Fig. 4. Change of population growth rate of Skeletonema cos-tatum exposed to diuron. Vertical bars represent means ± SD for three replicates. *p<0.05, **p<0.01 for control.
그림 Fig. 5. Concentration-response by irgarol (A) and diuron (B) treatment using the population growth rate of Skeletonema costatum.
표 Table 2. Population growth rate in the Skeletonema costatum exposed to irgarol and diuron
표 Table 3. Toxicity evaluation using population growth rate in the Skeletonema costatum exposed to irgarol and diuron (μg l^-1)

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