Song, Doo-won
(Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
,
Lee, Ga-won
(Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
,
Ro, Woong-bin
(Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
,
Kim, Heyong-seok
(Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
,
Kang, Hyun-min
(Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
,
Kim, Jong-won
(Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
,
Park, Soo-bin
(Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
,
Moon, Yang-seon
(Scholl of Korean Medicine, Dongshin University)
,
Na, Chang-su
(Nawoori (Ltd))
,
Park, Hee-myung
(Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
Kyungokgo-gamibang, Kyungokgo with Iksuyongjingo and Sparassis crispa, is a traditional Korean medicine used for restorative effects. This study aimed to evaluate the safety of Kyungokgo-gamibang in healthy beagle dogs. In the single-dose oral toxicity study, three beagle dogs were orally administer...
Kyungokgo-gamibang, Kyungokgo with Iksuyongjingo and Sparassis crispa, is a traditional Korean medicine used for restorative effects. This study aimed to evaluate the safety of Kyungokgo-gamibang in healthy beagle dogs. In the single-dose oral toxicity study, three beagle dogs were orally administered 2,000, 1,000, and 500 mg/kg of Kyungokgo-gamibang and were observed for 14 days. In the repeated-dose oral toxicity study, nine healthy dogs were orally administered 0.2g/kg of Kyungokgo-gamibang (n = 3, low-dose group), 1 g/kg of Kyungokgo-gamibang (n = 3, high-dose group), or normal saline (n = 3, control group) twice a day for 8 weeks. The hematological, serum biochemical, urine, protein, and lipid profiles were evaluated to investigate the adverse effects of the Kyungokgo-gamibang. During the study period, the dogs demonstrated no clinical signs and the hematological, serum biochemical, urine, protein, and lipid analyses revealed unremarkable findings. The study results suggest that Kyungokgo-gamibang can be safely administered to dogs without any adverse effects.
Kyungokgo-gamibang, Kyungokgo with Iksuyongjingo and Sparassis crispa, is a traditional Korean medicine used for restorative effects. This study aimed to evaluate the safety of Kyungokgo-gamibang in healthy beagle dogs. In the single-dose oral toxicity study, three beagle dogs were orally administered 2,000, 1,000, and 500 mg/kg of Kyungokgo-gamibang and were observed for 14 days. In the repeated-dose oral toxicity study, nine healthy dogs were orally administered 0.2g/kg of Kyungokgo-gamibang (n = 3, low-dose group), 1 g/kg of Kyungokgo-gamibang (n = 3, high-dose group), or normal saline (n = 3, control group) twice a day for 8 weeks. The hematological, serum biochemical, urine, protein, and lipid profiles were evaluated to investigate the adverse effects of the Kyungokgo-gamibang. During the study period, the dogs demonstrated no clinical signs and the hematological, serum biochemical, urine, protein, and lipid analyses revealed unremarkable findings. The study results suggest that Kyungokgo-gamibang can be safely administered to dogs without any adverse effects.
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제안 방법
Clinical signs, including vomiting, diarrhea, jaundice, hair loss, and stool abnormalities, were evaluated.
Hematological examinations were performed, including CBC tests (Vetscan HM2 Hematology system; Abaxis, Inc., Union City, CA, USA), serum chemistry panels (Cobas C Ⅲ instrument; Roche Diagnostics, South San Francisco, CA, USA), and electrolyte tests (9180 Electrolyte Analyzer; Roche Diagnostic, South San Francisco, CA, USA). The CBC test included tests to evaluate the white blood cell count, red blood cell count, hemoglobin level, hematocrit level, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, and platelet count.
In this study, hematological, serum chemical, protein, lipid, and urinary profiles were evaluated in healthy dogs before and after oral administration of Kyungokgo-gamibang for identifying the possible toxicity. In both the single-dose and repeated-dose oral administration toxicity studies, the results of CBC tests, serum chemistry analysis, electrolyte tests, SPE, and LPE did not show significant changes and no clinical signs were observed after the administration of Kyun- gokgo-gamibang in all groups.
Kyungokgo-gamibang extract, used as the test substance in this study, was provided by Nauri after setting the material and mixing ratio.
, Union City, CA, USA), serum chemistry panels (Cobas C Ⅲ instrument; Roche Diagnostics, South San Francisco, CA, USA), and electrolyte tests (9180 Electrolyte Analyzer; Roche Diagnostic, South San Francisco, CA, USA). The CBC test included tests to evaluate the white blood cell count, red blood cell count, hemoglobin level, hematocrit level, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, and platelet count. Serum chemistry profiles and electrolyte tests included tests to evaluate the levels of blood urea nitrogen levels, creatinine, glucose, alanine ami- notransferase, aspartate aminotransferase, alkaline phospha- tase, gamma-glutamyl transferase, total bilirubin, lactate de- hydrogenase, total cholesterol, triglyceride, amylase, lipase, total protein, albumin, creatine kinase, uric acid, calcium, phosphate, sodium, and potassium.
For the repeated-dose oral toxicity study, the Kruskal-Wallis test was used for comparisons of values among the three groups, followed by the Mann-Whitney test and post hoc test. The Wilcox on rank test was used to compare the results before and after the Kyungokgo-gamibang administration in each group. A p value of <0.
Adverse effects, including clinical signs and mortality, were observed for 14 days after the first oral administration. The toxicity of the test substance was evaluated at 0, 1, 3, 7, and 14 days after the first administration through physical and laboratory examination, serum protein electrophoresis (SPE), and lipoprotein electrophoresis (LPE).
Dogs in the control group were administered normal saline orally twice daily for 8 weeks. The toxicity of the test substance was evaluated at 0, 2, 4, 6, and 8 weeks after the first administration through physical and laboratory examination, SPE, and LPE.
This study demonstrated the safety of Kyungokgo-gamib- ang through single-dose and repeated-dose oral administration in dogs for the first time. Single-dose oral administration of Kyungokgo-gamibang up to 2,000㎎/㎏ is considered safe, while long-term oral administration does not cause any adverse effects in dogs.
대상 데이터
The study included three Beagle dogs. The highest dose of the test substance was based on the maximum dose range used in the single-dose toxicity test of healthy functional foods.
이론/모형
Statistical analysis was performed using a commercial software, SPSS version 20 (IBM, Chicago, IL, USA). For the repeated-dose oral toxicity study, the Kruskal-Wallis test was used for comparisons of values among the three groups, followed by the Mann-Whitney test and post hoc test. The Wilcox on rank test was used to compare the results before and after the Kyungokgo-gamibang administration in each group.
성능/효과
In this study, the CBC test results did not change after the administration of Kyungokgo-gamibang owing to the involvement of healthy dogs without immune suppression. Similar to the results of this study, no significant change in CBC results were observed after administration of Kyungokgo in a previous human study including healthy participants.
The study results confirmed that Kyungokgo-gamibang can be safely administered without causing major side effects for a period of 8 weeks. However, there might be a few side effects that did not appear in the tests performed in this study, and the results were not assessed after administration for >8 weeks.
Two weeks after a single-dose oral administration, BW, BT, HR, and RR were within the normal range in all dogs (Table 1). No dogs died or demonstrated any clinical signs.
후속연구
However, there might be a few side effects that did not appear in the tests performed in this study, and the results were not assessed after administration for >8 weeks. Moreover, since this study was conducted in healthy dogs with normal clinical test values, safety in dogs with various diseases such as liver or kidney diseases should be further investigated. Therefore, for safely administering Kyungokgogamibang to dogs with various clinical conditions, collection of more data through various investigations in a larger population of dogs and long-term evaluations is required.
However, further scientific evaluation of the efficacy of Kyungokgo-gamibang is needed for applying it to veterinary medicine. The scientific data obtained from this study provide safety information about the traditionally used Korean medicine Kyungokgo-gamibang, which could be the foundation for further research in dogs.
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