[논문]B16F10 세포에서의 오크라 추출물의 미백 활성 검증

유단희; 이인철

doi:10.48022/mbl.2107.07006

B16F10 세포에서의 오크라 추출물의 미백 활성 검증
Whitening Effect of Abelmoschus esculentus on Melanoma Cells (B16F10) 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.49 no.4, 2021년, pp.485 - 492

유단희 (서원대학교 융복합대학) , 이인철 (서원대학교 바이오코스메틱학과)

초록
AI-Helper

본 연구는 오크라 추출물의 미백 효과를 검증하여 화장품 소재로서 활용가능성을 확인하고자 하였다. 먼저, 오크라 열수 및 70% 에탄올 추출물의 미백효과를 tyrosinase의 효소 억제 활성으로 측정한 결과, 최종 농도인 1,000 ㎍/ml 농도에서 22.2%, 32.8%의 저해활성 효과를 보였다. 세포 차원에서 미백효과를 측정하기 위해 오크라 열수 및 에탄올 추출물의 세포 생존율을 melanoma cell (B16F10)에서 MTT assay법을 이용하여 측정하였다. 그 결과, 오크라 열수 및 70% 에탄올 추출물에서 100 ㎍/ml 농도에서 95% 이상의 생존율을 보였으며, 세포 독성이 나타나지 않은 농도 이하에서 멜라닌 생합성을 확인하기 위해 실험을 진행하였으며, 농도 의존적으로 멜라닌 합성을 저해하는 것을 확인하였다. 오크라 열수 및 70% 에탄올 추출물의 단백질 발현억제 효과를 5, 10, 50, 100 ㎍/ml의 농도에서 western blot으로 측정하였으며, 양성대조군으로 β-actin을 사용하였다. 그 결과, 오크라 열수 추출물은 100 ㎍/ml 농도에서 MITF, tyrosinase, TRP-1, TRP-2 인자들은 각각 88.1%, 24.8%, 62.2%, 42.9%의 효과를 나타내었다. 오크라 70% 에탄올 추출물은 100 ㎍/ml 농도에서 MITF, tyrosinase, TRP-1, TRP-2 인자들은 각각 65.3%, 58.3%, 66.2%, 65.3%의 효과를 나타내었다. 결론적으로 오크라 열수 및 70% 에탄올 추출물의 미백 효과가 검증되었으며, 기능성 화장품 소재로서 활용가능성을 확인하였다.

Abstract ▼ AI-Helper

In this study, the whitening effect of Abelmoschus esculentus extract was investigated to confirm its applicability in cosmetics. To determine the whitening effect, the tyrosinase-inhibitory activity of Abelmoschus esculentus hot water extract (AEWE) and 70% ethanol extract (AEEE) was measured. At the final concentration of 1000 ㎍/ml, AEWE showed an inhibitory activity of 22.2% and AEEE of 32.8%. To determine the whitening effect at the cellular level, the viability of melanoma cells treated with AEWE and AEEE was evaluated using the MTT assay. At concentrations of 100 ㎍/ml or less, both AEWE- and AEEE-treated groups showed cell survival rates of >95%. Furthermore, in both AEWE- and AEEE-treated melanoma cells, the melanin content decreased in a concentration-dependent manner. The inhibitory effects of AEWE and AEEE used at 5, 10, 50, and 100 ㎍/ml on protein expression were measured by western blot, with β-actin as the positive control. At a concentration of 100 ㎍/ml, AEWE showed an inhibitory effect of 88.1%, 24.8%, 62.2%, and 42.9% on microphthalmia-associated transcription factors (MITF), tyrosinase, tyrosinase-related proteins (TRP)-1, and TRP-2 factors, respectively. At the same concentration, AEEE showed inhibitory effect of 65.3%, 58.3%, 66.2%, and 65.3% against MITF, tyrosinase, TRP-1, and TRP-2 factors, respectively. In conclusion, the whitening effects of AEWE and AEEE were verified, and their applicability as a natural ingredient in cosmetics was confirmed.

주제어

표/그림 (5)

그림 Fig. 1. Inhibition rate of extract from Abelmoschus esculentus on tyrosinae.
그림 Fig. 2. Cell viability of extract from Abelmoschus esculentus on melanoma cells (B16F10). After B16F10 cells (1 × 10⁵ cells/ well) were started in medium for 24 h the cells were treated with 5, 10, 50, 100, 500 and 1,000 μg/ml of AEWE and AEEE for 24 h. Result are means ± SD. of triplicate data. (Significant as compared to control. *p < 0.05, **p< 0.01, ***p< 0.001).
그림 Fig. 3. Inhibition melanin synthesis of extract from Abelmoschus esculentus on melanoma cell (B16F10). B16F10 cells (1 × 10⁵ cells/well) were treated with AEWE and AEEE and α-MSH (1 μg/ ml) for 24 h. Each value represents mean ± SD of three individual experiments. (Significant as compared to α-MSH-treated group. *p < 0.05, **p < 0.01, ***p < 0.001)
그림 Fig. 4. MITF, tyrosinase, TRP-1 and TRP-2 protein expression rate of hot water extract from Abelmoschus esculentus on melanoma cell (B16F10). B16F10 cells (1 × 10⁵ cells/well) were incubated for 24 h in DMEM medium containing 10% FBS were treated with various concentrations of AEWE with α-MSH (1 μg/ml) for 24 h. Then total protein was isolated. MITF, tyrosinase, TRP-1 and TRP-2 protein level was determined by western blot. Each value represents mean ± SD of three individual experiments. (Significant as compared to α-MSH-treated group. *p< 0.05, **p < 0.01, ***p < 0.001)
그림 Fig. 5. MITF, tyrosinase, TRP-1 and TRP-2 protein expression rate of 70% ethanol extract from Abelmoschus esculentus on melanoma cell (B16F10). B16F10 cells (1 × 10⁵ cells/well) were incubated for 24 h in DMEM medium containing 10% FBS were treated with various concentrations of AEEE with α-MSH (1 μg/ml) for 24 h. Then total protein was isolated. MITF, tyrosinase, TRP-1 and TRP-2 protein level was determined by western blot. Each value represents mean ± SD of three individual experiments. (Significant as compared to α-MSH-treated group. *p<0.05, **p < 0.01, ***p < 0.001)

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