[논문]팥 껍질 추출물의 미백 기능성 활성 검증

이수연; 오민정; 염현지; 이진영

doi:10.48022/mbl.2111.11011

팥 껍질 추출물의 미백 기능성 활성 검증
A Study of Whitening Functional Activity Verification from Red Bean (Phaseolus angularis) Shell Extract 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.50 no.2, 2022년, pp.277 - 282

이수연 (대구한의대학교 바이오산업융합학부) , 오민정 (호서대학교 화장품생명공학부) , 염현지 (호서대학교 화장품생명공학부) , 이진영 (호서대학교 화장품생명공학부)

초록
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본 연구는 한국, 중국 등 극동아시아의 온대지역에서 재배되고 우리나라에서는 콩 다음으로 수요가 많은 팥 껍질(Phaseolus angularis shell)의 미백 효과를 검증하여 화장품 소재로서의 가능성을 확인하고자 하였다. 팥 껍질의 효능을 screening 하기 위하여 효소 실험인 tyrosinase 저해활성을 측정한 결과 1,000 ㎍/ml의 농도에서 76.39%의 저해활성을 나타내었다. 이후 멜라노마 세포의 생존율을 측정하여 팥 껍질이 세포에 독성을 미치지 않는 생존율 90% 이상의 농도에서 세포 실험을 진행하였다. 팥 껍질 추출물이 멜라닌 합성에 영향을 미치는 인자인 MITF, TRP-1, TRP-2, tyrosinase 단백질 및 mRNA 발현 억제 효과를 측정한 결과 모든 인자에서 발현량이 감소하였으며 대조군으로 사용된 kojic acid와 비교하였을 때 발현 정도가 유의하거나 더 감소한 것을 확인할 수 있었다. 이러한 결과, 팥 껍질 추출물의 미백 활성이 우수하다는 것을 확인할 수 있었으며 이는 미백 기능성화장품 소재로서 활용 가능성이 있을 것으로 사료된다.

Abstract ▼ AI-Helper

Whitening effect of the extract of Phaseolus angularis (PA) shell was investigated for its potential application as a functional ingredient for cosmetic products. The tyrosinase inhibitory effect, which is related to whitening, was 76.4% at a concentration of 1,000 ㎍/ml. Cell viability of melanoma cells was measured to test toxicity of the PA shell extract at concentrations showing at least 90% viability. In addition, western blot and RT-PCR assays of the PA shell extract showed concentration-dependent decreases of MITF, TRP-1, TRP-2 and tyrosinase protein and inhibitory effect of mRNA expression. These findings suggested that extract from PA shell has great potential as a cosmeceutical ingredient with whitening effect.

주제어

표/그림 (7)

그림 Fig. 1. Inhibition rate of extract from Phaseolus angularis shell on tyrosinase. Result are means ± SD. of triplicate data.
표 Table 1. Sequence of the Primers Used for RT-PCR.
그림 Fig. 2. Cell viability of extract from Phaseolus angularis shell on melanoma cell (B16F10). Result are means ± SD. of triplicate data.
그림 Fig. 3. MITF protein and mRNA expression rate of extract from Phaseolus angularis shell on melanoma cell (B16F10). After B16F10 cells (5 × 10⁵ cells) were started in serum free medium for 1 h the cells were treated with 25, 50 and 100 μg/ml of Phaseolus angularis shell for 24 h. Each values represents mean ± SD of three individual experiments (**p < 0.01, ***p < 0.001).
그림 Fig. 4. TRP-1 protein and mRNA expression rate of extract from Phaseolus angularis shell on melanoma cell (B16F10). After B16F10 cells (5 × 10⁵ cells) were started in serum free medium for 1 h the cells were treated with 25, 50 and 100 μg/ml of Phaseolus angularis shell for 24 h. Each values represents mean ± SD of three individual experiments (**p < 0.01, ***p < 0.001).
그림 Fig. 5. TRP-2 protein and mRNA expression rate of extract from Phaseolus angularis shell on melanoma cell (B16F10). After B16F10 cells (5 × 10⁵ cells) were started in serum free medium for 1 h the cells were treated with 25, 50 and 100 μg/ml of Phaseolus angularis shell for 24 h. Each values represents mean ± SD of three individual experiments (**p < 0.01, ***p < 0.001).
그림 Fig. 6. Tyrosinase protein and mRNA expression rate of extract from Phaseolus angularis shell on melanoma cell (B16F10). After B16F10 cells (5 × 105 cells) were started in serum free medium for 1 h the cells were treated with 25, 50 and 100 μg/ml of Phaseolus angularis shell for 24 h. Each values represents mean ± SD of three individual experiments (**p < 0.01, ***p < 0.001).

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