Kim, Juree
(Department of Plant Science and Technology, Chung-Ang University)
,
Choi, Jungwon
(Department of Plant Science and Technology, Chung-Ang University)
,
Kang, Sam Sik
(Natural Products Research Institute and College of Pharmacy, Seoul National University)
,
Lee, Sanghyun
(Department of Plant Science and Technology, Chung-Ang University)
Qantitative analysis of six compounds: (+)-catechin, benzoic acid, gallic acid methyl ester, paeonol, paeoniflorin, and albiflorin from Paeonia lactiflora extracts was performed using high-performance liquid chromatography and an ultraviolet (UV) detector, following different extraction methods. A r...
Qantitative analysis of six compounds: (+)-catechin, benzoic acid, gallic acid methyl ester, paeonol, paeoniflorin, and albiflorin from Paeonia lactiflora extracts was performed using high-performance liquid chromatography and an ultraviolet (UV) detector, following different extraction methods. A reverse-phase column was used in a gradient elution system, and UV detection was performed at 280 nm. The results showed that the quantity of paeoniflorin was the highest in ethanol and water extracts (73.89 and 57.87 mg/g, respectively) among the six compounds. This study contributes a good analysis method for the contents of P. lactiflora and would be propitious for developing medicines and functional foods.
Qantitative analysis of six compounds: (+)-catechin, benzoic acid, gallic acid methyl ester, paeonol, paeoniflorin, and albiflorin from Paeonia lactiflora extracts was performed using high-performance liquid chromatography and an ultraviolet (UV) detector, following different extraction methods. A reverse-phase column was used in a gradient elution system, and UV detection was performed at 280 nm. The results showed that the quantity of paeoniflorin was the highest in ethanol and water extracts (73.89 and 57.87 mg/g, respectively) among the six compounds. This study contributes a good analysis method for the contents of P. lactiflora and would be propitious for developing medicines and functional foods.
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제안 방법
Chromatographic analysis was performed using HPLC system (Agilent technology 1290 Infinity Ⅱ, Santa Clara, CA, USA) equipped with a pump, an auto-sampler, and a UV detector. Solvents used for HPLC (water and acetonitrile) were HPLC grade and purchased from J.
In this study, we aimed to quantify the phytochemical constituents in PL using a high-performance liquid chromatography (HPLC)-ultraviolet (UV) detector and compare the quantity of each compound in the extract after different extraction methods.
Quantitative analysis of the six representative compounds in EEP and WEP was performed using HPLC with a reverse phase column and gradient elution of solvents A and B in the mobile phase. The HPLC method showed good separation, and a wavelength of 280 nm was found to be effective for detection.
대상 데이터
Baker (Avantor, Radnor, PA, USA). Acetic acid (99.7%) was purchased from Samchun Pure Chemicals (Pyeongtaek, Korea). Six compounds: (+)-catechin, benzoic acid, gallic acid methyl ester, paeonol, paeoniflorin, and albiflorin (Fig.
7%) was purchased from Samchun Pure Chemicals (Pyeongtaek, Korea). Six compounds: (+)-catechin, benzoic acid, gallic acid methyl ester, paeonol, paeoniflorin, and albiflorin (Fig. 1) were obtained from Natural Product Institute of Science and Technology (www.nist.re.kr), Anseong, Korea.
The ethanolic (EtOH) (3-19-0091) and water (3-19-0048) extracts of PL were provided by Korea Institute of Oriental Medicine, Daejeon, Korea.
데이터처리
The data from the calibration curves of the standards are shown in Table 1. The calibration curves were constructed by linearly plotting the peak area against the prepared concentrations of the standard solutions and were analyzed using linear regression. The linear regression coefficients (r2) for the standards were between 0.
성능/효과
Another study showed that paeoniflorin was efficiently extracted with 70% MeOH or water [32], although not with pure EtOH or MeOH [33]. In this study, the quantity of paeoniflorin was higher in EEP than in WEP, which shows that the extraction using 70% EtOH is more suitable. Additionally, if reflux extraction had been used for the ethanolic extracion of PL, the extraction yield would have increased.
This seems to be due to the variation in yield according to the extraction method. The extraction yields of EEP and WEP were 16.63% (w/w) and 27.64% (w/w), respectively, which shows the extraction efficiency of water extraction method. Among the six compounds in PL, the quantity of paeoniflorin was remarkably high, followed by albiflorin, in both EEP and WEP.
Table 2 shows the quantity of the six compounds in EEP and WEP. The results showed that the quantity of the six compounds was generally higher in EEP than in WEP. However, considering the dry weights, the quantity of albiflorin, paeoniflorin, and benzoic acid was higher in WEP than in EEP.
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