[논문]홍삼·백년초 복합물이 관절염 개선에 미치는 효과

이승현; 이창현; 김홍준; 김영식; 신민지; 김지현; 마상용; 이세연; 권진; 오찬호

doi:10.14374/hfs.2021.29.2.81

[국내논문] 홍삼·백년초 복합물이 관절염 개선에 미치는 효과
Effects of red ginseng and Baeknyeoncho complex on the arthritis improvement 원문보기

大韓韓醫學方劑學會誌 = Herbal formula science, v.29 no.2, 2021년, pp.81 - 91

이승현 (우석대학교 식품생명공학과) , 이창현 (우석대학교 한의과대학) , 김홍준 (우석대학교 한의과대학) , 김영식 (우석대학교 한의과대학) , 신민지 (우석대학교 식품생명공학과) , 김지현 (우석대학교 식품생명공학과) , 마상용 (우석대학교 식품생명공학과) , 이세연 (우석대학교 한약학과) , 권진 (한국복지대학 의료보장구과) , 오찬호 (우석대학교 식품생명공학과)

Abstract ▼ AI-Helper

Objectives : This study was conducted to evaluate the efficacy of a complex mixture of natural substances of ginseng and baeknyeoncho on the arthritic rats. Methods : In vitro experiments were conducted to ensure the stability of the complex. After setting toxicity and concentration by MTT assay, the antioxidant effect was measured through DPPH and ABTS radical scavenging activity. To confirm the anti-inflammatory effects of the complex, levels of nitric oxide (NO) and pro-inflammatory cytokines (IL-1β, TNF-α) were measured in LPS-treated macrophage cell lines (RAW264.7). We injected monosodium iodoacetate (MIA) 50 μl (60 mg/ml) into knee joints of rats to induce osteoarthritis. The rats were divided into three groups (normal (n=5), control (n=5), and OR (n=5) group). The control group consumed 2 mg/kg of physiological saline once a day for 4 weeks, and the OR group was mixed at a concentration of 416.5 mg/kg of Baengnyeoncho (O) and 208.25 mg/kg of red ginseng (R) and ingested 1 mL each 5 days a week. Results : This complex increased the DPPH and ABTS radical scavenging rate. The complex decreased NO production and pro-inflammatory cytokine production of macrophages. In the OR group, the secretion of cytokine in serum was decreased. In histopathological examination, the joint tissue of the composite showed less damage to the synovial membrane, cartilage, and fibrous tissue than the control group. Conclusions : As a result of this study, natural complexes have antioxidant, anti-inflammatory and cartilage protection effects. Therefore, we expect the complex to be effective in treating osteoarthritis.

주제어

표/그림 (5)

그림 Figure 1. Effect of natural substances on cell viability in cultured RAW264.7 cells. (A) O (10-1000㎍/㎖) and R (10-1000㎍/㎖), (B) O (5, 100, 150㎍/㎖) and R (50, 100㎍/㎖) mixtures, (C) O (100㎍/㎖), R (50㎍/㎖) and OR mixture was treated into RAW 264.7 cells, cultured for 20 hours, and the cells were analyzed by the MTT method. Measured at 570nm using a microplate reader. Result are expressed as mean ± S.D. a-e: Different superscript letters mean significantly different between all the group. (p<0.05)
그림 Figure 2. Effects of O, R and OR on radical scavenging activity The radical scavenging activity of (A)DPPH, (B) ABTS is expressed as mean ± SD. a-e: Different superscript letters mean significantly different between all the group. (p<0.05)
그림 Figure 3. Effects of O, R and OR on (A) NO, (B) IL-1β, (C) TNF-α production in cultured RAW 264.7 cells (A): RAW 264.7 cell (2×104 cells/well) was treated with O (100㎍/㎖), R (50㎍/㎖) and OR mixture and LPS (1μg/ml) for 24h. (B), (C): RAW 264.7 cell (8×105 cells/well) was treated with O (100㎍/㎖), R (50㎍/㎖) and OR mixture and LPS (1μg/ml) for 24h. The results were expressed as mean±S.D. a-e: Different superscript letters mean significantly different between all the group. (p<0.05)
그림 Figure 4. Effects of OR on (A) IL-1β (B) TNF-α in the serum of MIA-induced osteoarthritis rats. N: Normal SD rat group, C: MIA-induced osteoarthritis rat group, OR: MIA-induced osteoarthritis rat group treated with OR (O: 416.5 mg/kg, R: 208.25mg/kg). The results were expressed as mean±S.D. a-e: Different superscript letters mean significantly different between all the group. (p<0.05)
그림 Figure 5. Effect of OR on joint pathology (A) Hematoxylin & Eosin(H&E), (B) Safranin-O in joint tissue of MIA-induced osteoarthritis rat. N: Normal SD rat group, C: MIA-induced osteoarthritis rat group, OR: MIA-induced osteoarthritis rat group treated with OR (O: 416.5 mg/kg, R: 208.25mg/kg).

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