[논문]매생이 추출물 첨가 통밀 쿠키의 마우스 혈당 항상성 개선 효과

임재민; 전수현; 정유진; 이광원

doi:10.9721/kjfst.2021.53.3.313

매생이 추출물 첨가 통밀 쿠키의 마우스 혈당 항상성 개선 효과
Improvement of blood glucose homeostasis in mice fed with Capsosiphon fulvescens extract-added whole wheat cookie 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.53 no.3, 2021년, pp.313 - 320

임재민 (고려대학교 일반대학원 생명공학과) , 전수현 (고려대학교 식품생의학안전연구소) , 정유진 (고려대학교 일반대학원 생명공학과) , 이광원 (고려대학교 일반대학원 생명공학과)

초록
AI-Helper

본 연구는 일반 쿠키(NC), 통밀 쿠키(WC) 그리고 매생이 추출물이 첨가된 통밀 쿠키(WCFE)를 제조하고 4주 동안 쿠키로만 이루어진 식이를 마우스에게 제공한 뒤 혈당과 관련된 지표들의 변화를 관찰하였다. 4주 섭취 이후 NC를 섭취한 그룹과 비교하여 WC와 WCFE를 섭취한 그룹에서 단기간의 체중 증가의 감소효과를 보였으며, 매생이 추출물의 첨가는 간과 신장의 스트레스 증가에 영향이 없음을 확인할 수 있었다. 공복 혈당 수준은 WC 그룹과 WCFE 그룹에서 NC 그룹에 비해 유의적으로 감소하였다(p<0.001). 혈청 인슐린 농도는 WCFE 그룹에서 유의적인 차이를 보이며 감소하였고(p<0.05), 이를 바탕으로 인슐린 저항성 관련 지표인 HOMA-IR를 산출 시 WCFE 그룹에서 유의적인 차이를 보이며 감소함으로써(p<0.05) 매생이 추출물이 첨가되었을 때 인슐린 저항성을 개선한 것으로 판단된다. 또한, OGTT의 30분대 혈당치에서 NC 그룹 및 WC 그룹과 비교하여 WCFE 그룹에서 혈당 증가 폭이 감소하는 것을 확인할 수 있었고, WCFE 그룹에서 가장 낮은 AUC 면적 값을 나타냈다. OGTT 및 AUC 결과를 종합해볼 때 일반 쿠키와 통밀 쿠키에 비해 매생이 추출물의 첨가로 인해 내당능에 도움을 줄 수 있을 것으로 판단된다. 그러나 혈당 관련 유전자인 GLUT2와 GLUT4의 경우, 통밀에 의해 발현이 증가하는 경향은 관찰되었으나 매생이 추출물 첨가에 의한 효과는 관찰되지 않았다. 또한 IRS-1의 경우 WC 그룹에 비해 WCFE 그룹의 발현이 증가하였고 PI3K p85α 발현의 경우 WC 그룹에 비해 WCFE 그룹의 발현이 감소하는 경향을 보여, 매생이 추출물의 첨가가 혈당 관련 유전자의 발현에는 영향을 미치지 못하는 것으로 보인다. 따라서, 이러한 결과를 종합했을 때 WCFE 섭취 시, 혈당 관련 유전자 발현에는 영향을 미치지는 못하지만, 내당능 개선 효과를 가지며 인슐린 저항성 개선 효과의 가능성을 보여 혈당 항상성 개선에 기여할 것으로 판단된다.

Abstract ▼ AI-Helper

The present study aimed to investigate the effect of whole wheat cookie supplemented with Capsosiphon fulvescens (CF) extract on serum glucose homeostasis in C57BL/6 mice. This study examined whether the same effect was demonstrated for whole wheat cookie in comparison to previous research documenting the glucose-lowering effect of food products combined with CF extract. Mice were divided into three groups depending on the diet administered: normal cookie (NC), whole wheat cookie (WC), and WC blended with CF extract (WCFE). After 4 weeks of administering the experimental diet, the blood glucose level, serum insulin level, and homeostatic model assessment for insulin resistance index were found to be significantly lower in the WCFE group than in the NC and WC groups. These results suggest that whole wheat cookie containing CF extract is effective in preventing insulin resistance and maintaining blood glucose homeostasis.

주제어

표/그림 (5)

표 Table 1. Ingredients and composition of cookies
그림 Fig. 1. Dietary effect of whole wheat cookie added with Capsosiphon fulvescens extract (WCFE) on body weight, relative organ weight and food intake in the mice fed for 4 weeks. (A) Changes of body weight and (B) Food intake during cookie diet. (C) Relative organ (liver and kidney) weight after sacrifice. NC, normal cookie; WC, whole wheat cookie; WCFE, WC blended with Capsosiphon fulvescens extract (CFE); six mice in each group. Data are represented as means ± standard deviation (SD). ns is not significantly different at p<0.05 by Duncan’s method.
그림 Fig. 2. Dietary effect of WCFE on fasting blood glucose, serum insulin level and homeostatic model assessment for insulin resistance (HOMA-IR). (A) Comparison of fasting blood glucose level between week 0 and week 4. (B) Concentration of serum insulin level. (C) HOMA-IR=(fasting blood glucose×fasting serum insulin) / 405. NC, normal cookie; WC, whole wheat cookie; WCFE, WC blended with Capsosiphon fulvescens extract (CFE); six mice in each group. Data are represented as means±SD. ***p<0.001 versus week 0 according to Student's t-test. ns is not significant compared to week 0 at p<0.05 according to Student's t-test. ^a-bMeans in a row by different superscripts are significantly different at the p<0.05 by Duncan's method.
그림 Fig. 3. Dietary effect of WCFE of oral glucose tolerance test (OGTT). (A) Changes of blood glucose levels while having the OGTT. (B) Area under the curve of blood glucose levels (AUC) after having the OGTT. NC, normal cookie; WC, whole wheat cookie; WCFE, WC blended with Capsosiphon fulvescens extract (CFE); six mice in each group. ^a-b Means in a row by different superscripts are significantly different at the p<0.05 by Duncan's method, ns is not significantly different at p<0.05 by Duncan's method.
그림 Fig. 4. Effect of WCFE on the expressions of (A) glucose transporter (GLUT) 2, (B) GLUT4, (C) insulin receptor substrate (IRS)-1 and (D) phosphoinositide 3-kinases (PI3K) p85α genes in C57BL/6 mice. NC, normal cookie; WC, whole wheat cookie; WCFE, WC blended with Capsosiphon fulvescens extract (CFE); six mice in each group. Data are represented as means±SD. ns is not significantly different at p<0.05 by Duncan's method.

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