[논문]무 품종의 세균성 무름병 저항성 생물검정법 평가

타니아 아프로즈; 허온숙; 노나영; 이재은; 황애진; 김빛샘; 아와리스 데비 아세파; 이주희; 성정숙; 이호선; 한범수

doi:10.5352/jls.2021.31.7.609

무 품종의 세균성 무름병 저항성 생물검정법 평가
Evaluation of Bioassay Methods to Assess Bacterial Soft Rot Resistance in Radish Cultivars 원문보기

생명과학회지 = Journal of life science, v.31 no.7, 2021년, pp.609 - 616

타니아 아프로즈 (국립농업과학원 농업유전자원센터) , 허온숙 (국립농업과학원 농업유전자원센터) , 노나영 (국립농업과학원 농업유전자원센터) , 이재은 (국립농업과학원 농업유전자원센터) , 황애진 (국립농업과학원 농업유전자원센터) , 김빛샘 (국립농업과학원 농업유전자원센터) , 아와리스 데비 아세파 (국립농업과학원 농업유전자원센터) , 이주희 (국립농업과학원 농업유전자원센터) , 성정숙 (국립식량과학원 남부작물부) , 이호선 (농촌진흥청 국제기술협력과) , 한범수 (국립농업과학원 농업유전자원센터)

초록
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세균성 무름병 균[Pectobacterium carotovorum subsp. carotovorum (Pcc)]에 의해서 일어나는 무름병은 아시아 국가에서 재배되는 무에 있어서 심각한 질병 중의 하나로 알려져 있다. 이번 연구의 목적은 상업적으로 시판되는 무 품종의 세균성 무름병의 저항성에 대한 효율적인 생물검정법을 확립하고자 하였다. 첫째, 무 품종에 대해 세균성 무름병의 효율적인 생물검정 방법을 조사하였다. 6개의 무 품종을 다양한 조건[두 가지 온도(25℃와 30℃), 3가지 접종방법(관주, 분무, 침지), 두 발생단계(2와 4잎 단계)]으로 조사하였다. 연구 결과는 무름병균 1×10⁶ cfu/ml 농도를 분무한 4잎 단계와 30℃에서 배양한 생물검정 방법이 무 품종에 대해 가장 효율적인 방법임을 알 수 있었다. 둘째, 41개의 무 품종에 5가지 세균(KACC 10225, KACC 10343, KACC 10421, KACC 10458, KACC 13953)을 접종하여 저항성을 조사하였다. KACC 10421가 세균성 무름병의 감수성 및 저항성 질병 정도를 가장 잘 나타냈다. 41개의 무 품종 중 13개는 무름병 균에 대해 중도 저항성을 나타냈고 28개는 감수성을 나타냈다. 이 연구에서 중도저항성 무 품종은 세균성 무름병 저항성 육종을 위한 저항성 자원으로 활용 가능하고 육종가, 농민, 연구자, 최종 소비자에 의해서 다양한 목적을 위해 사용 가능할 것으로 사료된다.

Abstract ▼ AI-Helper

Bacterial soft rot, caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), is one of the destructive diseases of radish (Raphanus sativus) in Asian countries. The objective of this study was to establish an efficient bioassay method for the evaluation of bacterial soft rot resistance in commercial radish cultivars. First, an efficient bioassay method for examining resistance to bacterial soft rot in commercial radish cultivars was investigated. Six commercial radish cultivars were tested under various conditions: two temperatures (25℃ and 30℃), three inoculations methods (drenching, spraying, and root dipping), and two growth stages (two- and four-leaf stages). The results suggested that spraying with 1×106 cfu/ml of bacterial inoculums during the four-leaf stage and incubating at 30℃ could be the most efficient screening method for bacterial soft rot resistance in commercial radish cultivars. Second, we investigated the degree of resistance of 41 commercial radish cultivars to five Pcc isolates, namely KACC 10225, KACC 10343, KACC 10421, KACC 10458, and KACC 13953. KACC 10421 had the strongest susceptibility in terms of moderately resistant disease response to bacterial soft rot. Out of the 41 radish cultivars, 13 were moderately resistant to this pathogen, whereas 28 were susceptible. The moderately resistant radish cultivars in this investigation could serve as resistance donors in the breeding of soft rot resistance or could be used to determine varietal improvement for direct use by breeders, scientists, farmers, researchers, and end customers.

주제어

표/그림 (5)

그림 Fig. 1. Disease index (0–4) of radish soft rot. a, DI = 0; b, DI = 1; c, DI = 2; d, DI = 3 and e, DI = 4. Scale bar, 4.8 cm.
그림 Fig. 2. Radish (Raphanus sativus) cultivar, Gmchorong (SAM1) showing soft rot symptoms at 25℃ (A) and 30°C (B) after inoculation by the drenching (a, d), spraying (b, e), and root-dipping (c, f) methods. Spraying and 30℃ resulted in the highest DI (DI 4). For drenching, a 10-ml sample of bacterial inoculum was drenched onto each plant; for spraying, a 10-ml sample of inoculum was sprayed onto each plant using a fine atomizer; for root dipping, each plant was dipped into a 50-ml sample of bacterial suspension for 20 min.
표 Table 1. Resistance of six commercial cultivars to Pectobacterium carotovorum subsp. carotovorum KACC 10421 by growth stage, inoculation method, and temperature
표 Table 2. Resistance of 41 commercial radish cultivars to five isolates of Pectobacterium carotovorum subsp. carotovorum^x
그림 Fig. 3. Radish (Raphanus sativus) Jeonmuhoomu (K1) (A) and Heongjaealtaimu (NS1) (B) exhibiting bacterial soft rot symptoms after inoculation with the Pcc isolates KACC 10225 (a, f), KACC 10343 (b, g), KACC 10421 (c, h), KACC 10458 (d, i), KACC 13953 (e, j). Among them, KACC 10421 exhibited the strongest resistance.

AI 본문요약
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대상 데이터

We investigated resistance among 41 commercial radish cultivars purchased from seed companies in Korea. All experiments were conducted in a greenhouse and growth chamber at the National Agrobiodiversity Center (NAC), National Institute of Agricultural Sciences, Jeonju, Republic of Korea.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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