[논문]곰보배추에 함유된 6-Hydroxyluteolin 7-O-Glucoside 분석을 위한 HPLC/DAD 분석법 밸리데이션

이학동; 바헤; 최중원; 김주리; 유아람; 배민정; 이상현

doi:10.22889/kjp.2021.52.3.186

곰보배추에 함유된 6-Hydroxyluteolin 7-O-Glucoside 분석을 위한 HPLC/DAD 분석법 밸리데이션
HPLC/DAD Method Validation of 6-Hydroxyluteolin 7-O-Glucoside Analysis from Salvia plebeia 원문보기

생약학회지, v.52 no.3, 2021년, pp.186 - 191

이학동 (중앙대학교 식물생명공학과) , 바헤 (중앙대학교 식물생명공학과) , 최중원 (중앙대학교 식물생명공학과) , 김주리 (중앙대학교 식물생명공학과) , 유아람 (중앙대학교 식물생명공학과) , 배민정 (한국식품산업클러스터진흥원) , 이상현 (중앙대학교 식물생명공학과)

Abstract ▼ AI-Helper

We developed a method to identify and quantify 6-hydroxyluteolin 7-O-glucoside in the powder of Salvia plebeia (PS) using high-performance liquid chromatography coupled with diode array detector (HPLC/DAD) and equipped with reverse-phase INNO C18 column. The analytical method was optimized and validated using novel parameters. The obtained values for the limits of detection and quantification were 3.60 and 10.90 ㎍/mL, respectively. Calibration curve showed good linearity in the concentration range tested (0.00625-0.1 mg/mL, r2 = 1.0000), high accuracy (96.2-101.4%), and precision values (RSD ≤ 0.27%). Our analysis support the use of our method for accurately identifying and quantifying 6-hydroxyluteolin 7-O-glucoside from PS in routine analyses and large-scale extraction processes for content determination.

주제어

표/그림 (6)

그림 Fig. 1. Chemical structure of 6-HLG (6-hydroxyluteolin 7-O-glucoside).
그림 Fig. 2. HPLC chromatograms and specificity of 6-HLG (A) and PS (B).
표 Table I. Linearity, LOD and LOQ of 6-HLG
표 Table II. Accuracy for the determination of 6-HLG
표 Table III. Intra-day precision for the determination of 6-HLG content
표 Table IV. Inter-day precision for the determination of 6-HLG content

AI 본문요약
AI-Helper

제안 방법

HPLC/DAD conditions−HPLC analysis was conducted using reverse-phase HPLC system with an INNO C18 column (25 cm × 4.6 mm, 5 μm)
plebeia extract (PS). This study validated the established analytical method of functional marker compoound 6-HLG of PS using HPLC/DAD, prior to developing a high value-added functional product using PS.

성능/효과

4%. Acceptable recovery values have been obtained indicating that the analytical method we used to calculate 6-HLG has achieved high accuracy.
The validation parameters employed in our analytical methodology showed excellent results in terms of the spec- ificity, precision, accuracy. The findings of our analysis support the use of our method for accurately identifying and quantifying 6-HLG from PS in routine analyses and large scale extraction processes for content determination. More- over, our study can provide the first steps in developing health functional products that could contribute in the pharmaceutical industrialization of PS.
The values obtained were lower than 2%, which meets the criteria recommended by the ICH guidelines. The results indicated that the analytical method to quantify 6-HLG derived from PS showed great reliability. Furthermore, when comparing the intra- and inter-day RSD mean values, a minor difference was observed between the two, but the latter values were slightly lower.

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