[논문]중금속 및 디젤 오염 토양에서 분리한 중금속 내성 식물 생장 촉진 근권세균의 특성

이수연; 이윤영; 조경숙

doi:10.48022/mbl.2106.06013

중금속 및 디젤 오염 토양에서 분리한 중금속 내성 식물 생장 촉진 근권세균의 특성
Characterization of Heavy Metal Tolerant and Plant Growth-Promoting Rhizobacteria Isolated from Soil Contaminated with Heavy Metal and Diesel 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.49 no.3, 2021년, pp.413 - 424

이수연 (이화여자대학교 환경공학과) , 이윤영 (이화여자대학교 환경공학과) , 조경숙 (이화여자대학교 환경공학과)

초록
AI-Helper

식물과 근권미생물을 이용해 토양 오염물질을 제거하는 rhizoremediation의 효율을 높이기 위해서는 오염물질을 제거함과 동시에 식물 생장을 촉진시키는 미생물 자원 개발이 필요하다. 본 연구에서는 중금속 및 유류 복합 오염 토양에서 서식하고 있는 옥수수와 톨페스큐의 근권으로부터 중금속(구리, 카드뮴 및 납) 내성을 가진 근권세균을 순수분리하였고, 식물 생장 촉진능, 중금속 내성능 및 디젤 분해능을 정성적으로 평가하였다. 그 결과 중금속 내성, 식물 생장 촉진 활성 및 디젤 분해능을 가진 6종의 균주를 분리하였다. 옥수수 근권에서 분리한 CuM5와 CdM2 균주는 Cupriavidus sp.로 동정되었다. 톨페스큐 근권에서 분리한 CuT6, CdT2, CdT5 및 PbT3는 각각 Fulvimonas soli, Cupriavidus sp., Novosphingonium sp. 및 Bacillus sp.로 동정되었다. Cupriavidus sp. CuM5와 CdM2는 중금속 내성과 디젤 분해능은 상대적으로 낮았으나, 식물 생장 촉진능이 상대적으로 우수하였다. 6종 중에서 디젤 분해능이 가장 우수한 균주는 Cupriavidus sp. CdT2와 Bacillus sp. PbT3이었다. 특히, Bacillus sp. PbT3는 3종의 중금속에 대해 상대적으로 우수한 내성을 가졌고 식물 생장 촉진능도 우수하였다. 본 연구에서 분리한 근권세균은 유류와 중금속 복합 오염 토양을 정화시키며 식물 생장을 촉진시키는 새로운 미생물 자원으로 활용 가능하다.

Abstract ▼ AI-Helper

In order to enhance rhizoremediation performance, which remediates contaminated soils using the interactions between plants and microorganisms in rhizosphere, it is required to develop effective microbial resources that simultaneously degrade contaminants and promote plant growth. In this study, heavy metal-resistant rhizobacteria, which had been cultivated in soils contaminated with heavy metals (copper, cadmium, and lead) and diesel were isolated from rhizospheres of maize and tall fescue. After that, the isolates were qualitatively evaluated for plant growth promoting (PGP) activities, heavy metal tolerance, and diesel degradability. As a result, six strains with heavy metal tolerance, PGP activities, and diesel degradability were isolated. Strains CuM5 and CdM2 were isolated from the rhizosphere soils of maize, and were identified as belonging to the genus Cupriavidus. From the rhizosphere soils of tall fescue, strains CuT6, CdT2, CdT5, and PbT3 were isolated and were identified as Fulvimonas soli, Cupriavidus sp., Novosphingobium sp., and Bacillus sp., respectively. Cupriavidus sp. CuM5 and CdM2 showed a low heavy metal tolerance and diesel degradability, but exhibited an excellent PGP ability. Among the six isolates, Cupriavidus sp. CdT2 and Bacillus sp. PbT3 showed the best diesel degradability. Additionally, Bacillus sp. PbT3 also exhibited excellent heavy metal tolerance and PGP abilities. These results indicate that the isolates can be used as promising microbial resources to promote plant growth and restore soils with contaminated heavy metals and diesel.

주제어

표/그림 (4)

표 Table 1. Comparison of viable cell numbers grown on heavy metal-containing plates inoculated with the rhizospheric soil suspensions.
표 Table 2. Plant growth promoting activities of heavy metal-tolerant rhizobacteria isolated from the rhizosphere soils.
그림 Fig. 1. Neighbor-Joining Phylogenetic trees of the strains CuM5, CdM2, CuT6, CdT2, CdT5 and PbT3 estimated from 16S rRNA sequence. Boostrap values (percentages of 1,000 replications) are shown at the branch points. The scale bar represents 0.1 substitutions per site. The 16S rRNA gene sequence of heavy metal tolerance bacteria and similar bacteria to six strains were used as outgroup.
표 Table 3. Activities of heavy metal tolerance and PGP by eight identified strains.

참고문헌 (63)

Kumar V, Pandita S, Singh Sidhu GP, Sharma A, Khanna K, Kaur P, et al. 2021. Copper bioavailability, uptake, toxicity and tolerance in plants: a comprehensive review. Chemosphere 262: 127810.

상세보기
Dias MC, Monteiro C, Moutinho-Pereira J, Correia C, Goncalves B, Santos C. 2013. Cadmium toxicity affects photosynthesis and plant growth at different levels. Acta Physiol. Plant. 35: 1281-1289.

상세보기
Zulfiqar U, Farooq M, Hussain S, Maqsood M, Hussain M, Ishfaq M, et al. 2019. Lead toxicity in plants: Impacts and remediation. J. Environ. Manage. 250: 109557.

상세보기
Joo JO, Kim IH, Oh B-K. 2014. Removal of Cupper(II), Zinc(II) in marine environment by heavy metal resistant Desulfovibrio desulfuricans. KSBB J. 29: 139-144.

원문보기 상세보기
Koo S, Cho K. 2007. Characterization of a heavy metal-resistant and plant growth-promoting rhizobacterium, Methylobacterium sp. SY-NiR1. Korean J. Microbiol. Biotechnol. 35: 58-65.
Benitez E, Melgar R, Nogales R. 2004. Estimating soil resilience to a toxic organic waste by measuring enzyme activities. Soil Biol. Biochem. 36: 1615-1623.

상세보기
Wohler, I. 1997. Auxin-indole derivatives in soils determined by a colorimetric method and by high performance liquid chromatography. Microbiol. Res. 152: 399-405.

상세보기
Nagarajkumar M, Bhaskaran R, Velazhahan R. 2004. Involvement of secondary metabolites and extracellular lytic enzymes produced by Pseudomonas fluorescens in inhibition of Rhizoctonia solani, the rice sheath blight pathogen. Microbiol. Res. 159: 73-81.

상세보기
Hwang JS, Song HG. 2020. Antifungal activity of Bacillus subtilis isolates against toxigenic fungi. Korean J. Microbiol. 56: 28-35.
Grobelak A, Kokot P, Swiatek J, Jaskulak M, Rorat A. 2018. Bacterial ACC deaminase activity in promoting plant growth on areas contaminated with heavy metals. J. Ecol. Eng. 19: 150-157.

상세보기
Dell'Amico E, Cavalca L, Andreoni V. 2005. Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metal-resistant, potentially plant growth-promoting bacteria. FEMS Microbiol Ecol. 52: 153-162.

상세보기
Barrado LN. 2018. Isolation and characterisation of endophytes from vitis vinifera. Univ Politecnica Valencia, pp. 1-25.
Lee YY, Seo Y, Ha M, Lee J, Yang H, Cho KS. 2021. Evaluation of rhizoremediation and methane emission in diesel-contaminated soil cultivated with tall fescue (Festuca arundinacea). Environ. Res. 194: 110606.

상세보기
Hajdu R, Slaveykova VI. 2012. Cd and Pb removal from contaminated environment by metal resistant bacterium Cupriavidus metallidurans CH34: Importance of the complexation and competition effects. Environ. Chem. 9: 389-398.

상세보기
Lyu Y, Zheng W, Zheng T, Tian Y. 2014. Biodegradation of polycyclic aromatic hydrocarbons by Novosphingobium pentaromativorans US6-1. PLoS One 9: 101438.
Deng Y, Yang F, Deng C, Yang J, Jia J, Yuan H. 2017. Biodegradation of BTEX aromatics by a haloduric microbial consortium enriched from a sediment of Bohai Sea, China. Appl. Biochem. Biotechnol. 183: 893-905.

상세보기
Babu AG, Kim JD, Oh BT. 2013. Enhancement of heavy metal phytoremediation by Alnus firma with endophytic Bacillus thuringiensis GDB-1. J. Hazard Mater. 250: 477-483.

상세보기
Jiang C, Sheng X, Qian M, Wang Q. 2008. Isolation and characterization of a heavy metal-resistant Burkholderia sp. from heavy metal-contaminated paddy field soil and its potential in promoting plant growth and heavy metal accumulation in metal-polluted soil. Chemosphere 72: 157-164.

상세보기
Hong S, Cho K-S. 2007. Effects of plants, rhizobacteria and physicochemical factors on the phytoremediation of contaminated soil. Korean J. Microbiol. Biotechnol. 35: 261-271.
Oh SD, Ahn BO, Kim MK, Sohn SI, Ryu TH, Cho HS, et al. 2010. Effects of protox herbicide tolerance rice cultivation on microbial community in paddy soil. Korean J. Env. Agric. 32: 95-101.

원문보기 상세보기
Dotaniya ML, Meena VD. 2015. Rhizosphere effect on nutrient availability in soil and its uptake by plants: a review. Proc. Natl. Acad. Sci. India Sect. B - Biol Sci. 85: 1-12.

상세보기
Ashraf R, Ali TA. 2007. Effect of heavy metals on soil microbial community and mung beans seed germination. Pakistan J. Bot. 39: 629.
Wang L, Wang J, Zhu L, Wang J. 2018. Toxic effects of oxytetracycline and copper, separately or combined, on soil microbial biomasses. Environ. Geochem. Health 40: 763-776.

상세보기
Kabagale AC, Cornu B, Van Vliet F, Meyer CL, Mergeay M, Simbi JBL, et al. 2010. Diversity of endophytic bacteria from the cuprophytes Haumaniastrum katangense and Crepidorhopalon tenuis. Plant Soil 334: 461-474.

상세보기
Xiao L, Yu Z, Liu H, Tan T, Yao J, Zhang Y, et al. 2020. Effects of Cd and Pb on diversity of microbial community and enzyme activity in soil. Ecotoxicology 29: 551-558.

상세보기
Prapagdee B, Watcharamusik A. 2009. Adaptive and cross-protective responses against cadmium and zinc toxicity in cadmium-resistant bacterium isolated from a zinc mine. Braz. J. Microbiol. 40: 838-845.
Estrada-De Los Santos P, Solano-Rodriguez R, Matsumura-Paz LT, Vasquez-Murrieta MS, Martinez-Aguilar L. 2014. Cupriavidus plantarum sp. nov., a plant-associated species. Arch Microbiol. 196: 811-817.

상세보기
Liu X, Wei S, Wang F, James EK, Guo X, Zagar C, et al. 2012. Burkholderia and Cupriavidus spp. are the preferred symbionts of Mimosa spp. in Southern China. FEMS Microbiol. Ecol. 80: 417-426.

상세보기
Yang C, Ho YN, Makita R, Inoue C, Chien MF. 2020. Cupriavidus basilensis strain r507, a toxic arsenic phytoextraction facilitator, potentiates the arsenic accumulation by Pteris vittata. Ecotoxicol. Environ. Saf. 190: 110075.

상세보기
Siripornadulsil S, Siripornadulsil W. 2013. Cadmium-tolerant bacteria reduce the uptake of cadmium in rice: potential for microbial bioremediation. Ecotoxicol. Environ. Saf. 94: 94-103.

상세보기
Vicentin RP, Santos JV, Labory CRG, Costa AM, Moreira FMS, Alves E. 2018. Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator. Rev. Bras. Cienc do Solo. 42: 1-12.
Siripornadulsil S, Thanwisai L, Siripornadulsil W. 2014. Changes in the proteome of the cadmium-tolerant bacteria Cupriavidus taiwanensis KKU2500-3 in response to cadmium toxicity. Can J. Microbiol. 60: 121-131.

상세보기
Taghavi S, Lesaulnier C, Monchy S, Wattiez R, Mergeay M, van der Lelie D. 2009. Lead(II) resistance in Cupriavidus metallidurans CH34: Interplay between plasmid and chromosomally-located functions. Antonie Van Leeuwenhoek, Int. J. Gen. Mol. Microbiol. 96: 171-182.
Huang N, Mao J, Hu M, Wang X, Huo M. 2019. Responses to copper stress in the metal-resistant bacterium Cupriavidus gilardii CR3: a whole-transcriptome analysis. J. Basic Microbiol. 59: 446-457.

상세보기
Chen WM, Wu CH, James EK, Chang JS. 2008. Metal biosorption capability of Cupriavidus taiwanensis and its effects on heavy metal removal by nodulated Mimosa pudica. J. Hazard Mater. 151: 364-371.

상세보기
Minari GD, Saran LM, Constancio MTL, da Silva RC, Rosalen DL, de Melo WJ, et al. 2020. Bioremediation potential of new cadmium, chromium, and nickel-resistant bacteria isolated from tropical agricultural soil. Ecotoxicol. Environ. Saf. 204: 111038.

상세보기
Li X, Yan Z, Gu D, Li D, Tao Y, Zhang D, et al. 2019. Characterization of cadmium-resistant rhizobacteria and their promotion effects on Brassica napus growth and cadmium uptake. J. Basic Microbiol. 59: 579-590.

상세보기
Wang Q, Ma L, Zhou Q, Chen B, Zhang X, Wu Y, et al. 2019. Inoculation of plant growth promoting bacteria from hyperaccumulator facilitated non-host root development and provided promising agents for elevated phytoremediation efficiency. Chemosphere 234: 769-776.

상세보기
Singh P, Kim YJ, Nguyen NL, Hoang VA, Sukweenadhi J, Farh MEA, et al. 2015. Cupriavidus yeoncheonense sp. nov., isolated from soil of ginseng. Antonie van Leeuwenhoek, Int. J. Gen. Mol. Microbiol. 107: 749-758.
Kuppusamy S, Thavamani P, Megharaj M, Lee YB, Naidu R. 2016. Polyaromatic hydrocarbon (PAH) degradation potential of a new acid tolerant, diazotrophic P-solubilizing and heavy metal resistant bacterium Cupriavidus sp. MTS-7 isolated from long-term mixed contaminated soil. Chemosphere 162: 31-39.

상세보기
Ledger T, Zuniga A, Kraiser T, Dasencich P, Donoso R, Perez-Pantoja D, et al. 2012. Aromatic compounds degradation plays a role in colonization of Arabidopsis thaliana and Acacia caven by Cupriavidus pinatubonensis JMP134. Antonie van Leeuwenhoek 101: 713-723.

상세보기
Kampfer P, Martin K, McInroy JA, Glaeser SP. 2015. Proposal of Novosphingobium rhizosphaerae sp. nov., isolated from the rhizosphere. Int. J. Syst. Evol. Microbiol. 65: 195-200.

상세보기
Krishnan R, Menon RR, Busse HJ, Tanaka N, Krishnamurthi S, Rameshkumar N. 2017. Novosphingobium pokkalii sp. nov, a novel rhizosphere-associated bacterium with plant beneficial properties isolated from saline-tolerant pokkali rice. Res. Microbiol. 168: 113-121.

상세보기
Islam MR, Sultana T, Joe MM, Yim W, Cho JC, Sa T. 2013. Nitrogen-fixing bacteria with multiple plant growth-promoting activities enhance growth of tomato and red pepper. J. Basic Microbiol. 53: 1004-1015.

상세보기
Vives-Peris V, Gomez-Cadenas A, Perez-Clemente RM. 2018. Salt stress alleviation in citrus plants by plant growth-promoting rhizobacteria Pseudomonas putida and Novosphingobium sp. Plant Cell Rep. 37: 1557-1569.

상세보기
Chettri B, Singh AK. 2019. Kinetics of hydrocarbon degradation by a newly isolated heavy metal tolerant bacterium Novosphingobium panipatense P5:ABC. Bioresour. Technol. 294: 122190.

상세보기
Liu X, Liu M, Chen X, Yang Y, Hou L, Wu S, et al. 2019. Indigenous PAH degraders along the gradient of the Yangtze Estuary of China: Relationships with pollutants and their bioremediation implications. Mar. Pollut. Bull. 142: 419-427.

상세보기
Segura A, Udaondo Z, Molina L. 2021. PahT regulates carbon fluxes in Novosphingobium sp. HR1a and influences its survival in soil and rhizospheres. Environ. Microbiol. 23: 2969-2991.

상세보기
Rodriguez-Conde S, Molina L, Gonzalez P, Garcia-Puente A, Segura A. 2016. Degradation of phenanthrene by Novosphingobium sp. HS2a improved plant growth in PAHs-contaminated environments. Appl. Microbiol. Biotechnol. 100: 10627-10636.

상세보기
Ke T, Zhang J, Tao Y, Zhang C, Zhang Y, Xu Y, et al. 2021. Individual and combined application of Cu-tolerant Bacillus spp. enhance the Cu phytoextraction efficiency of perennial ryegrass. Chemosphere 263: 127952.

상세보기
Kamaruzzaman MA, Abdullah SRS, Hasan HA, Hassan M, Othman AR, Idris M. 2020. Characterisation of Pb-resistant plant growth-promoting rhizobacteria (PGPR) from Scirpus grossus. Biocatal. Agric. Biotechnol. 23: 101456.

상세보기
Saharan BS, Verma S. 2014. Potential plant growth promoting activity of Bacillus licheniformis UHI(II)7. Int. J. Microb. Resour. Technol. 2: 22-27.
Akhtar N, Ilyas N, Yasmin H, Sayyed RZ, Hasnain Z, Elsayed AE, et al. 2021. Role of Bacillus cereus in improving the growth and Phytoextractability of Brassica nigra (L.) K. koch in chromium contaminated soil. Molecules 26: 1569.

상세보기
Wang C, Liu Z, Huang Y, Zhang Y, Wang X, Hu Z. 2019. Cadmium-resistant rhizobacterium Bacillus cereus M4 promotes the growth and reduces cadmium accumulation in rice (Oryza sativa L.). Environ. Toxicol. Pharmacol. 72: 103265.

상세보기
Guo H, Luo S, Chen L, Xiao X, Xi Q, Wei W, et al. 2010. Bioremediation of heavy metals by growing hyperaccumulaor endophytic bacterium Bacillus sp. L14. Bioresour. Technol. 101: 8599-8605.

상세보기
Heidari P, Panico A. 2020. Sorption Mechanism and Optimization Study for the Bioremediation of Pb(II) and Cd(II) Contamination by Two Novel Isolated Strains Q3 and Q5 of Bacillus sp. Int. J. Environ. Res. Public Health 17: 4059.

상세보기
Singh S, Kumar V, Sidhu GK, Datta S, Dhanjal DS, Koul B, et al. 2019. Plant growth promoting rhizobacteria from heavy metal contaminated soil promote growth attributes of Pisum sativum L. Biocatal. Agric. Biotechnol. 17: 665-671.

상세보기
Pandey S, Ghosh PK, Ghosh S, De TK, Maiti TK. 2013. Role of heavy metal resistant Ochrobactrum sp. and Bacillus spp. strains in bioremediation of a rice cultivar and their PGPR like activities. J. Microbiol. 51: 11-17.

상세보기
Al-Sharidah A, Richardt A, Golecki JR, Dierstein R, Tadros MH. 2000. Isolation and characterization of two hydrocarbon-degrading Bacillus subtilis strains from oil contaminated soil of Kuwait. Microbiol. Res. 155: 157-164.

상세보기
Kebria DY, Khodadadi A, Ganjidoust H, Badkoubi A, Amoozegar MA. 2009. Isolation and characterization of a novel native Bacillus strain capable of degrading diesel fuel. Int. J. Environ. Sci. Technol. 6: 435-442.

상세보기
Patowary K, Saikia RR, Kalita MC, Deka S. 2015. Degradation of polyaromatic hydrocarbons employing biosurfactant-producing Bacillus pumilus KS2. Ann. Microbiol. 65: 225-234.

상세보기
Yuniarti E, Dalmacio IF, Paterno ES. 2019. Heavy metal-resistant rhizobacteria from gold mine in Pongkor Indonesiaa and copper mine in Marinduque Philippines. J. ILMU Pertan. 31: 75-88.
Huang Y, Li L. 2014. Biodegradation characteristics of naphthalene and benzene, toluene, ethyl benzene, and xylene (BTEX) by bacteria enriched from activated sludge. Water Environ. Res. 86: 277-284.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증