[논문]천연물 복합 추출물 (HAE-06)의 기관지 확장에 미치는 영향

김대성; 김혜윰

doi:10.9799/ksfan.2021.34.5.545

천연물 복합 추출물 (HAE-06)의 기관지 확장에 미치는 영향
Effect of Natural Product Complex Extract (HAE-06) on Bronchial Dilation 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.34 no.5, 2021년, pp.545 - 552

김대성 (한풍제약 연구부) , 김혜윰 (원광대학교 한방심신증후군연구센터)

Abstract ▼ AI-Helper

HAE-06 extract is a mixture of four medicinal plants, namely Lonicerae Folium et Caulis (Lonicera japonica), Scutellariae Radix (Scutellaria baicalensis), Adenophorae Radix (Adenophora triphylla var. japonica), and Polygonati Oddorati Rhizoma (Polygonatum odoratum var. pluriflorum). The HAE-06 extract demonstrated a concentration-dependent relaxing effect and enhanced cAMP production in bronchial smooth muscle that had been stimulated to contract with acetylcholine. Using a blocker, it was confirmed that the effect was through the β2-adrenergic receptor/cAMP/PKA pathway. In addition, it is thought that the HAE-06 extract has a bronchial smooth muscle relaxation effect by reducing the inflow of Ca2+ through the K+ and Ca2+ channels present in the sarcoplasmic membrane. If research continues in the future, it is believed that it will be possible to use it as a material for pharmaceuticals and functional foods.

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표/그림 (9)

표 Table 1. Mixing ratio of HEA-06 extract
표 Table 2. HPLC gradient conditions for the measurement of baicalin and baicalein in Scutellariae Radix
표 Table 3. HPLC gradient conditions for the measurement of loganin, oganic acid, and sweroside in Lonicera japonica
그림 Fig. 1. HPLC chromatograms showing peaks corresponding to the marker compounds, baicalin and baicaein (A); loganin, loganic acid, and sweoside (B) of HEA-06 extract. HPLC, high performance liquid chromatography.
그림 Fig. 2. Dose-response curves by HEA-6 treatment in acetylcholine-induced bronchi. (A) Representative response physiological activity diagram by HEA-06 dose-dependent treatment in intact rat bronchi. (B) HEA-06 extract treatment has bronchial dilatation effect in the state of bronchoconstriction by acetylcholine 100 μM (B) (n=8).
그림 Fig. 3. Dose-response curves by HEA-6 treatment in acetylcholine-induced bronchi. Bronchial relaxation effect of HEA-06 treated with (A) non-selective β-adrenergic receptor antagonist, Pro (10 μM). pro, propranolol. Data are expressed as mean±standard error of the mean (n=5). *p<0.05, ***p< 0.001 vs. vehicle.
그림 Fig. 4. Dose-response curves by HEA-6 treatment in acetylcholine-induced bronchi. Bronchial relaxation effect of HEA-06 treated with (A) adenylyl cyclase inhibitor and (B) PKA inhibitor. Forsk, forskolin 10 μM, inhibitor of adenylyl cyclase; SQ, SQ22536 100 μM, selective inhibitor of protein kinase A. Data are expressed as mean±standard error of the mean (n=4). *p<0.05, **p<0.01, ***p<0.001 vs. vehicle.
그림 Fig. 5. Effect of cAMP-PKA pathway activation by treatment on HAE-06 extract. (A) The amount of cAMP produced by HAE-06 concentration in the bronchi was compared and shown as a graph. (B) Dose-response curves on the bronchodilation induced by HEA-06 in the acetylcholine- contracted treca in the absence or presence of selective inhibitor of protein kinase A, KT (10 or 100 μM). cAMP, cyclic adenosine monophosphate; KT, KT5720. Data are expressed as mean±standard error of the mean (n=5). *p<0.05, **p<0.01, ***p<0.001 vs. vehicle.
그림 Fig. 6. Dose-response curves for HEA-06-induced bronchodilation in acetylcholine contractile bronchi in the absence or presence of blockers. Treatment results of non-selective K⁺ channel blockers TEA(100 μM) and 4-AP(100 μM)(A) and L-type Ca²⁺ channel blockers Vera(1 μM) and Dilt(1 μM)(B). TEA, tetraethylammonium; 4-AP, 4-aminopyridine; Vera, verapamil; Dilt, diltiazem. Data are expressed as mean±standard error of the mean. *p<0.05, **p<0.01 vs. vehicle. Data are expressed as mean±standard error of the mean (n=4). *p<0.05, **p<0.01, ***p<0.001 vs. vehicle.

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동의어: Packet Collision Rate

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