[논문]갯씀바귀(Ixeris repens) 추출물의 항산화성 및 생리활성

김주성; 이연지; 김지윤; 최지원; 유선재; 김용태

doi:10.5657/kfas.2021.0742

갯씀바귀(Ixeris repens) 추출물의 항산화성 및 생리활성
Comparison of Antioxidant and Physiological Activities in Various Solvent Extracts of Ixeris repens 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.54 no.5, 2021년, pp.742 - 750

김주성 (군산대학교 식품생명공학전공) , 이연지 (군산대학교 식품생명공학전공) , 김지윤 (군산대학교 식품생명공학전공) , 최지원 (군산대학교 식품생명공학전공) , 유선재 (군산대학교 환경공학과) , 김용태 (군산대학교 식품생명공학전공)

Abstract ▼ AI-Helper

Ixeris repens is a type of halophyte that grows in high salinity sands found in coastal sand dunes and sandy shores. This study was conducted to investigate the contents, antioxidant potency, and physiological activities of I. repens. In analyses of general composition, carbohydrate, protein, ash, and moisture content were 57.42%, 10.48%, 11.99% and 10.29%, respectively. Potassium, calcium, sodium, and magnesium were its most prevalent minerals. The solvents used to extract I. repens were 70% ethanol, 80% methanol, and distilled water. Among the resultant extracts, ethanol and methanol extracts displayed higher total polyphenol and flavonoid contents than the water extract. ABTS (IC50, 0.12 mg/mL) and FRAP (0.77 mM) radical scavenging activity were highest in the water extract, while methanol extract exhibited the strongest DPPH radical scavenging activity (IC50, 1.32 mg/mL), NO scavenging activity (IC50, 4.10 mg/mL), and reducing power (EC50, 0.14 mg/mL). Tyrosinase, elastase, and α-glucosidase inhibitory activities were highest in the ethanol extract. The ethanol extract also possessed the most potent acetylcholinesterase inhibitory activity. These results indicate that I. repens may be useful as an antioxidant, and a functional substance in food and pharmaceutical materials.

주제어

표/그림 (7)

표 Table 1. Proximate composition of Ixeris repens (%)
표 Table 2. Mineral contents of Ixeris repens (mg/100 g)
표 Table 3. Comparison of the extraction yields and color values of various solvent extracts from Ixeris epens
표 Table 4. Total polyphenol and flavonoid contents of various solvent extracts from Ixeris repens
표 Table 5. Antioxidant activities of various solvent extracts from Ixeris repens
그림 Fig. 1. Acetylcholinesterase (AChE) and α-glucosidase inhibitory activities of various solvent extracts from Ixeris repens. The values are expressed as the mean±SD (n=3 per group).
표 Table 6. Tyrosinase and elastase inhibitory activities of various solvent extracts from Ixeris repens

AI 본문요약
AI-Helper

문제 정의

따라서 본 연구에서는 수분이 부족하고 염분이 다량 함유된 해안사구에 서식하는 염생식물인 갯씀바귀의 항산화성 및 생리 기능성을 구명하기 위하여 갯씀바귀를 증류수, 에탄올 및 메탄올을 용매로 사용하여 갯씀바귀 추출물을 제조하여 이화학적 특성, 항산화활성 및 생리활성을 비교 분석하여 항산화 및 생리 기능성 소재개발의 기초자료로 제공하고자 하였다.

제안 방법

각 시료의 AChE 저해 활성은 Ellman(1961)의 방법을 다소 수정하여 측정하였다. 96 well plate에 100 mM phosphate buffer(pH 8.
갯씀바귀 분말을 각각 3가지 용매(증류수, 70% ethanol, 80% methanol)를 사용하여 갯씀바귀 추출물을 제조하였다. 증류수를 용매로 사용한 추출은 갯씀바귀 50 g에 증류수를 시료 대비 20배의 양을 첨가하여 autoclave(121℃)에서 3시간 동안 가열 추출하였다.

대상 데이터

본 실험에 사용된 갯씀바귀(Ixeris repens)는 2020년 6-7월 충청남도 서천군 연안에서 채취하여 실험실로 운반한 후 흐르는 물로 수세하여 염분과 협잡물을 제거한 다음 자연 건조한 후 전체를 분쇄기(FM700SS; Hanil, Seoul, Korea)로 곱게 분쇄하여 추출물 제조에 사용하였다. 항산화활성 및 생리활성을 측정하기 위하여 Folin-Ciocalteu’s reagent, gallic acid, quercetin, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2, 2-diphenyl-1-picrylhydrazyl, vitamin C, mushroom tyrosinase, 3, 4-dihydroxy-L-phenylalamine (L-DOPA), kojic acid, angiotensin I-converting enzyme (ACE), hippuryl-his-leu (HHL) acetate salt, captopril, acetylcholinesterase (AChE), acetylthiocholine iodide (ATC), 5, 5’-dithio-bis-2-nitrobenzoic acid (DTNB) 등은 Sigma-Aldrich Co.

데이터처리

각 시료 간의 유의성 검정은 분산분석(ANOVA)을 한 후 P<0.05 수준에서 Duncan’s multiple range test에 따라 분석하여 시료 간 유의적 차이를 검증하였다.
실험 결과는 SPSS 22.0 package program(SPSS Inc., Chicago, IL, USA)으로 통계처리하여 3회 측정한 값의 평균±표준편차로 나타내었다

참고문헌 (37)

Ali MY, Kim DH, Seong SH, Kim HR, Jung HA and Choi JS. 2017. α-Glucosidase and protein tyrosine phosphatase 1B inhibitory activity of plastoquinones from marine brown alga Sargassum serratifolium. Mar Drugs 15, 368. https://doi.org/10.3390/md15120368.

상세보기
AOAC (Association of Official Analytical Chemists). 1990. Official methods of analysis. In: Association of Official Analytical Chemists. 15th ed. Arlington, VA, U.S.A., 777-788.
Benzie IFF and Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 239, 70-76. https://doi.org/10.1006/abio.1996.0292.

상세보기
Byun HG and Kim SK. 2005. Development of physiological activity and health functional materials of seaweed. Susanyeon-gu 22, 1-10.
Cho EK and Choi YJ. 2010. Physiological activities of hot water extracts from Ecklonia cava kjellman. J Life Sci 20, 1675-1682. https://doi.org/10.5352/JLS.2010.20.11.1675.

원문보기 상세보기
Choi JH, Kim JS, Jo BS, Kin J, Park HJ, An BJ, Kim M and Cho YJ. 2011. Biological activity in functional cosmetic purple sweet potato extracts. Kor J Food Preserv 18, 414-422. https://doi.org/10.11002/kjfp.2011.18.3.414.

원문보기 상세보기
Ellman GL, Courtney KD, Andres V Jr and Feather-stone RM. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7, 88-95. https://doi.org/10.1016/0006-2952(61)90145-9.

상세보기
Hwang ST, Kim SK, Na JG, Lee JS and Choi D. 2013. Isolation and characterization of expansion genes in a halophyte Suaeda japonica. J Life Sci 23, 182-189. https://doi.org/10.5352/JLS.2013.23.2.182.

원문보기 상세보기
Iida K, Hase K, Shimomura K, Sudo S, Kadota S and Namba T. 1995. Potent inhibitors of tyrosinase activity and melanin biosynthesis from Rheum officinale. Planta Med 61, 425-428. https://doi.org/10.1055/s-2006-958129.

상세보기
Jeon MJ, Kim M, Jang HJ, Lee SW, Kim JH, Kim HS and Lee SH. 2012. Whitening effect of Hizikia fusiformis ethanol extract and its fractions. J Life Sci 22, 889-896. http://dx.doi.org/10.5352/JLS.2012.22.7.889.

원문보기 상세보기
Jiratchayamaethasakul C, Ding Y, Hwang O, Im ST, Jang Y, Myung SW, Lee JM, Kim HS, Ko SC and Lee SH. 2020. In vitro screening of elastase, collagenawse, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals. Fish Aquat Sci 23, 6. https://doi.org/10.1186/s41240-020-00149-8.

원문보기 상세보기
Kim SS and Cha HC. 2017. Comparison of the total phenolic and flavonoid contents and antioxidant activities of four kinds of sand dune plants living in Taean, Korea. Korean J Plant Res 30, 8-16. https://doi.org/10.7732/kjpr.2016.30.1.008.

원문보기 상세보기
Kim PH, Kim MJ, Kim JH, Lee JS, Kim KH, Kim HJ, Jeon YJ, Heu MS and Kim JS. 2014. Nutritional and physiologically active characterizations of the sea squirt Halocynthia roretzi sikhae and the seasoned sea squirt. Korean J Fish Aquat Sci 47, 1-11. https://doi.org/10.5657/KFAS.2014.0001.

원문보기 상세보기
Kim MJ, Kim JS, Cho MA, Kang WH, Jeong DM and Ham SS. 2002a. Biological activity of Ixeris dentata Nakai juice extracts. J Korean Soc Food Sci Nutr 31, 924-930. https://doi.org/10.3746/jkfn.2002.31.5.924.

원문보기 상세보기
Kim MJ, Kim JS, Jeong DM, Ham SS and Yu CY. 2002b. Effect of antioxidant, antimutagenicity and anticancer of root extract from Ixeris dentata Nakai. Korean J Med Crop Sci 10, 222-229.
Kim S. 1995. Inhibitory effects of Ixeris dentata on the mutagenicity of aflatoxin B1, N-methyl-N'-nitro-N-nitrosoguanidine and the growth of MG-63 human osteosarcoma cells. J Kor Soc Food Nutr 24, 305-312.
Kraunsoe JA, Claridge TD and Lowe G. 1996. Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry 35, 9090-9096. https://doi.org/10.1021/bi953013b.

상세보기
Lee KS, Kim GH, Kim HH, Kim ES, Park HM and Oh MJ. 2009. Physiological functionalities of tea thermally processed from Ixeris dentata root. J Korean Soc Food Sci Nutr 38, 496-501. https://doi.org/10.3746/jkfn.2009.38.4.496.

원문보기 상세보기
Lee SH. 2011. Current status and prospect of nutraceuticals from marine algae. Bull Food Tech 24, 165-175.
Lee SY. 2014 Analysis of skin mechanism and an application for functional cosmetic from red bean Phaseolus angularis shell extract. Ph.D. Dissertation, Hoseo University, Asan, Korea.
Lee YJ, Jeon YJ and Kim YT. 2020. Comparison of antioxidant and physiological activities of various solvent extracts from Hizikia fusiformis. Korean J Fish Aqua Sci 53, 886-893. https://doi.org/10.5657/KFAS.2020.0886.

원문보기 상세보기
Lee YJ, Kim WS, Lee BJ, Jeon YJ and Kim YT. 2017. Quality characteristics and antioxidant activities of gruel containing Saccharina japonica powder. Korean J Fish Aquat Sci 50, 707-713. https://doi.org/10.5657/KFAS.2017.0707.

원문보기 상세보기
Lee YJ, Lim SY, Kim WS and Kim YT. 2016. Processing and quality characteristics of glutinous barley gruel containing Hizikia fusiformis. Korean J Fish Aquat Sci 49, 310-316. https://doi.org/10.5657/KFAS.2016.0310.

원문보기 상세보기
Lim S. 1996. A Sensory evaluation of the bitter compounds from Ixeris dentata Nakai. J Korean Soc Food Cook Sci 12, 115-121.
Middleton E and Kandaswami C. 1994. Potential health-promoting properties of citrus flavonoids. Food Technol 48, 115-119.
Moreno MI, Isla MI, Sampietro AR and Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71, 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0.

상세보기
Oyaizu N, Yasumizu R, Miyama-Inaba M, Nomura S, Yoshida H, Miyawaki S, Shibata Y, Mitsuoka S, Yasunaga K and Morii S. 1988. (NZW x BXSB) F1 mouse. A new animal model of idiopathic thrombocytopenic purpura. J Exp Med 167, 2017-2022. https://doi.org/10.1084/jem.167.6.2017.

상세보기
Re R, Pellegrini N, Proteggente A, Pannala A, Yang Min and Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Bio Med 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3.

상세보기
Seto M, Miyase T and Fukushima S. 1986. Sesquiterpene lactones from Ixeris dentata Nakai. Chem Pharm Bull 34, 4170-4176. https://doi.org/10.1248/cpb.34.4170.

상세보기
Shetty K, Curtis OF, Levin RE, Witkowsky R and Ang V. 1995. Prevention of vitrification associated with in vitro shoot culture of oregano. (Origanum vulgare) by Pseudomonas spp. J Plant Physiol 147, 447-451. https://doi.org/10.1016/S0176-1617(11)82181-4.

상세보기
Soka T. 1985. Dictionary of Chinese drugs. In: Shanghai science technology Shogakukan, eds. Shogakukan Press, Tokyo, Japan, 2243-2246.
Son HJ, Um MY, Kim I, Cho S, Han D and Lee C. 2016. In vitro screening for anti-dementia activities of seaweed extracts. J Korean Soc Food Sci Nutr 45, 966-972. https://doi.org/10.3746/jkfn.2016.45.7.966.

원문보기 상세보기
Song JE, Song JH, Cho SM, Min GH and Lee JS. 2010. Nutritional characteristics and physiological functionality of antidementia acetylcholinesterase inhibitor-containing methanol extract from Sorghum bicolor. Korean J Food Nutr 23, 226-232.

원문보기 상세보기
Talesa VN. 2001. Acetylcholinesterase in Alzheimer's disease. Mech Ageing Dev 122, 1961-1969. https://doi.org/10.1016/S0047-6374(01)00309-8.

상세보기
Watanabe J, Kawabata J, Kurihara H and Niki R. 1997. Isolation and identification of α-glucosidase inhibitors from Tochucha Eucommia ulmoides. Biosci Biotechnol Biochem 61, 177-178. https://doi.org/10.1271/bbb.61.177.

상세보기
Yang X. 2011. Biological activities of various halophytes and structure elucidation of antioxidative compounds in Suaeda japonica. M.S. Thesis, Mokpo University, Mokpo, Korea.
Yook C. 1997. Colored medicinal plants. Korea Academybook, Seoul, Korea, 588-589.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증