[논문]율피 추출물 및 분획물의 항산화 활성

박찬휘; 강현; 이성규

doi:10.5010/jpb.2022.49.2.150

율피 추출물 및 분획물의 항산화 활성
Antioxidant activity of ethanol extracts and fractions from Castanea crenata inner shell 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.49 no.2, 2022년, pp.150 - 154

박찬휘 (단국대학교 보건복지대학 임상병리학과) , 강현 (단국대학교 보건복지대학 임상병리학과) , 이성규 (단국대학교 보건복지대학 임상병리학과)

초록
AI-Helper

본 연구에서는 율피 추출물 및 분획물의 항산화 활성을 in vitro실험을 통하여 조사하였다. 총 폴리페놀 함량은 율피의 70% 에탄올 추출물에서 39.76 ㎍/mL였고 분획물 중에서는 ethyl acetate 층의 함량이 323.92 ㎍/mL으로 가장 높았다(p < 0.05). 총 플라보노이드 함량 측정 결과 폴리페놀 측정 결과와 유사하게 ethyl acetate 분획물의 함량이 13.12 ㎍/mL으로 가장 높았다(p < 0.05). DPPH 라디칼 소거능 측정 결과 ethyl acetate 분획물의 RC50 값이 2.27 ㎍/mL으로 양성 대조군인 ascorbic acid보다 낮은 RC50 값을 나타내었다(p < 0.05). 모든 결과를 종합해보았을 때, 율피의 70% 에탄올 추출물과 분획물 중 ethyl acetate 분획물의 항산화 활성이 가장 높게 나타났음을 확인하였다. 이에 본 연구의 결과는 율피를 기능성 소재로서 활용하는데 기초 자료로 쓰일 것으로 기대되며, ethyl acetate 분획물에 대한 추가적인 연구가 이루어져야 할 것으로 판단된다.

Abstract ▼ AI-Helper

This study examined the antioxidant activity of ethanol extract and fraction from Castanea crenata inner shell in vitro. Antioxidant activity was evaluated through total polyphenol and flavonoid contents and DPPH radical scavenging ability using electron spin resonance (ESR). The total polyphenol contents of the 70% ethanol extract and ethyl acetate fraction were 39.76 mg GAE/g and 323.92 mg GAE/g (p < 0.05). The total polyphenol contents of the ethyl acetate fraction were higher than other fractions. Furthermore, the total flavonoid contents of ethyl acetate were higher than other fractions at 13.12 mg QE/g (p < 0.05). In DPPH radical scavenging ability measurement, the RC50 value of ethyl acetate was lower than ascorbic acid at 2.27 ㎍/mL (p < 0.05). In conclusion, this result showed that the ethyl acetate fraction of Castanea crenata inner shell 70% ethanol extract has high antioxidant activity.

주제어

표/그림 (4)

$Fig 1 Diagram of Castanea crenata inner shell fraction preparation$ 그림 Fig 1 Diagram of Castanea crenata inner shell fraction preparation
$Fig 2 Total polyphenol contents in ethanol extracts and fractions from Castanea crenata inner shell. The total polyphenol content was analyzed as gallic acid equivalents (GAE) mg/g of extracts' fractions. Data were expressed as mean ± standard deviation (n = 3). Different letters indicate significant differences at p < 0.05 using Duncan's multiple range test$ 그림 Fig 2 Total polyphenol contents in ethanol extracts and fractions from Castanea crenata inner shell. The total polyphenol content was analyzed as gallic acid equivalents (GAE) mg/g of extracts' fractions. Data were expressed as mean ± standard deviation (n = 3). Different letters indicate significant differences at p < 0.05 using Duncan's multiple range test
$Fig 3 Total flavonoid contents in ethanol extracts and fractions from Castanea crenata inner shell. Total flavonoid content analyzed as quercetin equivalents (QE) mg/g of extracts fractions. Data were expressed as mean ± standard deviation (n = 3). Different letters indicate significant differences at p < 0.05 using Duncan's multiple range test$ 그림 Fig 3 Total flavonoid contents in ethanol extracts and fractions from Castanea crenata inner shell. Total flavonoid content analyzed as quercetin equivalents (QE) mg/g of extracts fractions. Data were expressed as mean ± standard deviation (n = 3). Different letters indicate significant differences at p < 0.05 using Duncan's multiple range test
$Fig 4 DPPH radical scavenging activity of ethanol extracts and fractions from Castanea crenata inner shell. The DPPH radical scavenging activity was measured using an ESR spectrophotometer. Graph and ESR spectra indicate the DPPH radical scavenging effect of (A) ascorbic acid, (B) 70% ethanol extracts, (C) hexane fractions, (D) chloroform fractions, (E) ethyl acetate fractions, (F) butanol fractions, and (G) water fractions. Meanwhile, (H) indicates the RC50 value of DPPH radical scavenging activity. Data were expressed as mean ± standard deviation (n = 3). Different letters indicate significant differences at p < 0.05 using Duncan's multiple range test$ 그림 Fig 4 DPPH radical scavenging activity of ethanol extracts and fractions from Castanea crenata inner shell. The DPPH radical scavenging activity was measured using an ESR spectrophotometer. Graph and ESR spectra indicate the DPPH radical scavenging effect of (A) ascorbic acid, (B) 70% ethanol extracts, (C) hexane fractions, (D) chloroform fractions, (E) ethyl acetate fractions, (F) butanol fractions, and (G) water fractions. Meanwhile, (H) indicates the RC50 value of DPPH radical scavenging activity. Data were expressed as mean ± standard deviation (n = 3). Different letters indicate significant differences at p < 0.05 using Duncan's multiple range test

참고문헌 (25)

Brieger K, Schiavone S, Miller Jr FJ, Krause KH (2012) Reactive oxygen species: from health to disease. Swiss medical weekly, 142:w13659

상세보기
Cha SH, Heo SJ, Jeon YJ (2006) Screening for DPPH Free Radical Scavenging Activities of Autogenous Seaweeds in Jeju Island Using a Electron Spin Resonance (ESR) Spectroscopy. Journal of Marine Bioscience and Biotechnology 1:252-259
Choi MO, Kim BJ, Jo SK, Jung HK, Lee JT, Kim HY, Kweon DJ (2013) Anti-allergic activities of Castanea crenata inner shell extracts fermented by Lactobacillus bifermentans. Korean Journal of Food Preservation 20:583-591
Cizmarova B, Birkova A, Hubkova B, Bolerazska B (2021) Pycnogenol - extract from French maritime pine bark (Pinus pinaster), as an effective antioxidant against superoxide radical. Functional Food Science 1:14-22
Frankel EN (1996) Antioxidants in lipid foods and their impact on food quality. Food chemistry 57:51-55

상세보기
Galanakis CM, Goulas V, Tsakona S, Manganaris GA, Gekas V (2013) A Knowledge Base for The Recovery of Natural Phenols with Different Solvents. International Journal of Food Properties 16:382-396

상세보기
Ganesan K, Xu B (2017) Polyphenol-Rich Lentils and Their Health Promoting Effects. International journal of molecular sciences 18:2390

상세보기
Garcia-Conesa MT, Larrosa M (2020) Polyphenol-Rich Foods for Human Health and Disease. Nutrients 12:400

상세보기
Gu YR, Kim JH, Hong JH (2018) The anti-oxidant, whitening and anti-wrinkle effects of Castanea crenata inner shell extracts processed by enzyme treatment and pressurized extraction. Korean Journal of Food Preservation 25:79-89
He W, Liu Y, Wamer WG, Yin JJ (2014) Electron spin resonance spectroscopy for the study of nanomaterial-mediated generation of reactive oxygen species. Journal of Food and Drug Analysis 22:49-63

상세보기
Jang MJ, Cheon SJ, Kim HY, Kwon DJ, Kim SH, Lee JT (2011) The Anti-Wrinkle and Whitening Effect of Exracts og Castanea crenata Inner Shell. Journal of Life Science 21:734-738
Kim CY, Kim JW, Jeong JS, Lim JO, Ko JW, Kim TW (2022) Inner Shell of Chestnut (Castanea crenatta) Supresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model. Nutrients 14:2067
Kim JY, Kim SY, Kwon HM, Kim CH, Lee SJ, Park SC, Kim KH (2014) Comparison of Antioxidant and Anti-Inflammatory Activity on Chestnut, Chestnut Shell and Leaves of Castanea crenata Extracts. Korean Journal of Medicinal Crop Science. The Korean Society of Medicinal Crop Science 22:8-16
Kim KC, Eom TK, Kim JS (2019) Polyphenol Contents and Antioxidant Activity of Fruit Extracts from Daphniphyllum macropodum. Journal of Advanced Engineering and Technology 12:149-155
Kim SJ, Kim GH (2020) Plants from Ulleung island ameliorate lipid accumulation and oxidative stress in oleic acid-induced in HepG2 Cells. Korean Journal of Food Preservation 27:817-828

상세보기
Kim, YJ, Kim SY, Jeong MJ, Lee UT, Choo ST, Youn SN, Kim MR (2018) Antioxidant effect of ethanol extract from Plantaginis Herba. The Korea Journal of Herbology 33:37-43
Kwon EJ, Kim YC, Kwon MS, Kim CS, Kang WW, Lee JB, Chung SK (2001) Antioxidative Activity of Solvent Fraction and Isolation of Antioxidative Compound from Chestnut Husk. Journal of the Korean Society of Food Science and Nutrition 30:726-731
Le DD, Nguyen DH, Zhao BT, Min BS, Song SW, Woo MH (2019) Quantitation and Radical Scavenging Activity Evaluation of Iridoids and Phenylethanoids from the Roots of Phlomis umbrosa (Turcz.) using DPPH Free Radical and DPPH-HPLC Methods. and their Cytotoxicity. Natural Product Sciences. 25:122-129

원문보기 상세보기
Lee JY, Surh JH (2021) Effect of the Addition of Chestnut Inner Shell Powder on the Physicochemical and Sensory Attributes of Yanggaeng. Korean Journal of Food and Cookery Science 37:31-40
Lee MY, Yoo MS, Whang YJ, Jin YJ, Hong MH, Pyo YH (2012) Vitamin C, Total Polyphenol, Flavonoid Contents and Antioxidant Capacity of Several Fruit Peels. Korean Journal of Food Science and Technology 44:540-544
Lee YS, Joo EY, Kim NW (2005) Antioxidant Activity of Extracts from the Lespedeza bicolor. Korean Journal of Food Preservation 12:75-79
Nawaz H, Shad MA, Rehman N, Andaleeb H, Ullah N (2020) Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds. Brazilian Journal of Pharmaceutical Sciences 56:e17129
Noh JR, Kim YJ. Gang GT, Hwang JH, Lee HS, Ly SY, Oh WK, Song KS, Lee CH (2011) Hepatoprotective effects of chestnut (Castanea crenata) inner shell extract against chronic ethanol-induced oxidative stress in C57BL/6 mice. Food and Chemical Toxicology 49:1537-1543

상세보기
Park CH, Kim KH, Yook HS (2014) Free Radical Scavenging Ability and Quality Characteristics of Yanggaeng Combined with Grape Juice. The Korean Journal of Food And Nutrition 27:596-602
Sajid M, Channakesavula CN, Stone SR, Kaur P (2021) Synthetic Biology towards Improved Flavonoid Pharmacokinetics. Biomolecules 11:754

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증