[논문]A431 skin cancer cell에서 Esculetin의 MAPKs pathway를 통한 항암 효과

성진영; 김용민

doi:10.22889/kjp.2022.53.4.181

A431 skin cancer cell에서 Esculetin의 MAPKs pathway를 통한 항암 효과
Esculetin Suppresses the Growth and Proliferation of A431 Skin Cancer Cells via the MAPKs Pathway 원문보기

생약학회지, v.53 no.4, 2022년, pp.181 - 191

Abstract ▼ AI-Helper

As the incidence of skin cancer increases every year, non-surgical treatment methods for cancer are being sought. Esculetin, a natural dihydroxy coumarin, is attracting attention as a therapeutic agent for certain diseases, such as cancer, based on its broad pharmacological activity. In this study, the anticancer ability of esculetin was evaluated using the epidermoid carcinoma cell line A431. As a result of evaluating the apoptosis ability of esculetin by MTT assay, apoptosis was observed in a time-concentration-dependent manner regardless of the presence or absence of FBS. As a result of quantitative real-time PCR, esculetin reduced cyclin D1 mRNA in a time-concentration-dependent manner. In addition, as a result of western blotting, esculetin significantly inhibited phosphorylation of ERK, JNK, and p38 in a concentration-dependent manner. The results of this study suggest that esculetin has the potential to be used as an effective natural medicine for the treatment of skin cancer.

주제어

표/그림 (11)

표 Table I. Gene name and assay ID number in real-time qPCR analysis
표 Table II. Primer sequences in real-time qPCR analysis
그림 Fig. 1. Esculetin suppresses skin cancer cell viability and growth. Cell viability was assessed for 24, 48, and 72 h in the (A) 0% FBS or (B) 10% of FBS using serum-free RPMI with increasing concentrations of esculetin (0.2, 0.4, 0.8 and 1 mM). Controls were treated with serum-free RPMI without or with 10% FBS, respectively. Indicates a significant difference from the control (*p < 0.05, **p < 0.01, ***p < 0.001).
표 Table III. Antibody name and assay ID number in western blotting analysis
그림 Fig. 2. Esculetin suppresses cyclin D1 at the mRNA level in a time and concentration dependent manner. (A) Esculetin 0.8 mM was treated with serum-free RPMI to A431 cells for 1, 2, 4 and 8 hours. Control treated only the serum-free RPMI. (B) Esculetin (0.2, 0.4 and 0.8 mM) was treated with serum-free RPMI in A431 cells for 1 hour in either 0% FBS or 10% of FBS conditions. Controls were treated with serum-free RPMI without or with 10% FBS, respectively. Indicates a significant difference from the control (*p < 0.05, **p < 0.01, ***p < 0.001).
그림 Fig. 3. Esculetin suppresses skin cancer cell cycle progression and induces apoptosis in a time dependent manner. (A) Esculetin 0.8 mM was treated with serum-free RPMI to A431 cells for 1, 4, 8, 20 and 24 hours. Control treated only the serum-free RPMI. (B) Analysis data using image J. Indicates a significant difference from the control (***p < 0.001).
그림 Fig. 4. Esculetin suppresses skin cancer cell cycle progression and induces apoptosis in a concentration dependent manner. (A) Esculetin (0.2, 0.4 and 0.8 mM) was treated with serum-free RPMI in A431 cells for 1 hour (cyclin D1) or 24 hour (caspase-3 and cleaved caspase-3) in 0% FBS conditions. (B) Analysis data for 0% FBS condition using Image J. (C) Esculetin (0.2, 0.4 and 0.8 mM) was treated with serum-free RPMI in A431 cells for 1 hour(cyclin D1) or 24 hour(caspase-3 and cleaved caspase-3) in 10% of FBS conditions. (D) Analysis data for 10% FBS condition using Image J. Controls were treated with serum-free RPMI without or with 10% FBS, respectively. Indicates a significant difference from the control (**p < 0.01, ***p < 0.001).
그림 Fig. 4. Continued
그림 Fig. 5. Esculetin consequently suppresses skin cancer through the MAPKs pathway. (A) Esculetin (0.2, 0.4 and 0.8 mM) was treated with serum-free RPMI in A431 cells for 15 minutes in 0% FBS conditions. (B) Analysis data for 0% FBS condition using Image J. (C) Esculetin (0.2, 0.4 and 0.8 mM) was treated with serum-free RPMI in A431 cells for 15 minutes in 10% FBS conditions. (D) Analysis data for 10% FBS condition using Image J. Controls were treated with serum-free RPMI without or with 10% FBS, respectively. Indicates a significant difference from the control (***p < 0.001).
그림 Fig. 5. Continued
그림 Fig. 6. Esculetin can suppresses the growth and proliferation of skin cancer by inhibiting the MAPKs pathway.

AI 본문요약
AI-Helper

문제 정의

본 연구에서는 피부암 세포 A431에서 esculetin의 항암 효과를 확인하였다. Esculetin은 A431에서 FBS 유무에 상관없이 시간 및 농도 의존적으로 apoptosis를 유발하였다.

데이터처리

모든 데이터는 평균 ± 표준편차로 표시하였으며, 통계적 유의수준은 *p <0.05, **p<0.01, ***p<0.001로 검증하였다
통계 분석 − 본 연구 데이터는 3 회 반복 실험하였으며 student t-test를 이용하여 비교 분석하였다

후속연구

Apoptosis 유도제인 caspase-3의 활성화는 esculetin 처리 후 24 시간에 나타났다. 더불어 esculetin의 농도에 따른 cyclin D1의 감소와 cleaved caspase-3의 발현증가, MAPKs의 유의한 감소는 esculetin이 피부암 치료를 위한 효과적인 천연 의약품으로 사용될 가능성이 있음을 시사한다.

참고문헌 (21)

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