[논문]베트남 후피향 추출물의 생리활성 검증에 대한 연구

정수현; 염현지; 오민정; 이진영

doi:10.15230/scsk.2022.48.1.39

베트남 후피향 추출물의 생리활성 검증에 대한 연구
A Study on the Verification of Physiological Activities of Ternstroemia kwangtungensis Merr. Extract 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.48 no.1, 2022년, pp.39 - 46

정수현 (호서대학교 화장품생명공학부) , 염현지 (호서대학교 화장품생명공학부) , 오민정 (호서대학교 화장품생명공학부) , 이진영 (호서대학교 화장품생명공학부)

초록
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본 연구에서는 기능성 소재로서 베트남 후피향 추출물의 활용 가치를 알아보고자 하였다. 후피향 추출물의 Nrf2 발현 정도를 측정한 결과, 50% 에탄올을 이용하여 후피향 잎을 추출한 추출물 200 ㎍/mL 농도에서 가장 높은 licuferase의 활성도를 보였다. 상온에서 시간 경과에 따른 추출물의 안정성을 확인하고자 UPLC를 실시하였으며, 자외선 스펙트럼 패턴의 양상이 동일하여 안정성이 유지됨을 확인하였다. 후피향 추출물의 항산화 효능을 확인하기 위해 전자공여능 및 ABTS⁺ radical 소거능을 측정한 결과 농도가 증가함에 따라 활성이 증가했고 1,000 ㎍/mL에서 각각 94.6%, 90.8%의 높은 효과를 보였다. Tyrosinase 저해활성을 측정한 결과 1,000 ㎍/mL의 농도에서 24.4%의 저해능을 나타내었다. 또한 후피향 추출물의 elastase 저해활성을 알아본 결과, 농도 의존적으로 저해능이 증가하였으며 1,000 ㎍/mL에서 42.7%의 억제활성을 확인할 수 있었다. 이러한 결과를 통해 Ternstroemia kwangtungensis Merr. (TK) 추출물이 화장품 천연 소재로서의 이용가치가 있음을 확인하였다.

Abstract ▼ AI-Helper

In this study, we tried to find out the value of using Vietnamese Ternstroemia kwangtungensis Merr. (TK) extracts as a functional material. As a result of measuring the level of Nrf2 expression of TK, 50% ethanol extract TK leaf showed the highest activity at a concentration of 200 ㎍/mL. In order to confirm the stability of the extract over time at room temperature, UPLC was performed, and stability was maintained because the patterns of the UV spectrum pattern were the same. In order to confirm the antioxidant efficacy of TK, the activity increased as the concentration increased as a result of measuring electron donating and ABTS+ radical scavenging ability. At 1,000 ㎍/mL, they showed effects of 94.6% and 90.8%, respectively. As a result of measuring the tyrosinase inhibitory activity showed an inhibitory ability of 24.4% at a concentration of 1,000 ㎍/mL. In addition, as a result of examining the elastase inhibitory activity of the extract was increased in a concentration-dependent manner, and it was confirmed that the inhibitory activity was 42.7% at 1,000 ㎍/mL. From these results, it was confirmed that the TK extract was valuable as a natural material for cosmetics.

주제어

표/그림 (6)

그림 Figure 1. Level of Nrf2 expression on Ternstroemia kwangtungensis Merr. (TK) extracts. ▮CON: Control ▯1: Water extract of TK stem 20 μg/mL ▯2: Water extract of TK stem 200 μg/mL ▯3: Water extract of TK leaf 20 μg/mL ▮4: Water extract of TK leaf 200 μg/mL ▮5: 50% ethanol extract of TK stem 20 μg/mL ▮6: 50% ethanol extract of TK stem 200 μg/mL ▯7: 50% ethanol extract of TK leaf 20 μg/mL ▮8: 50% ethanol extract of TK leaf 200 μg/mL ▮SFN: Sulforaphane, Positive control. Result are means ± SD of triplicate data (**p < 0.01, ***p < 0.001).
그림 Figure 2. UPLC chromatogram with UV spectrum pattern of TK extracts. Old: Initial TK extract, New: Six-month-old TK extract. (A): UV chromatogram-area, (B): UV spectrum.
그림 Figure 3. Electron donating ability of extract from TK. Electron donating ability for TK extract and butylated hydroxy anisole from various concentration were determined with 0.2 mM DPPH ethanolic solution.▮ TK: Ternstroemia kwangtungensis Merr. ▯BHA: Butylated hydroxy anisole. Result are means ± SD of triplicate data (**p < 0.01, ***p < 0.001).
그림 Figure 4. A BTS⁺ radical scavenging ability of TK extract. ABTS⁺ radical scavenging ability for TK extract and butylated hydroxy toluene from various concentration were determined with ABTS⁺radical solution. ▮TK: Ternstroemia kwangtungensis Merr. ▯BHT: Butylated hydroxy toluene. Result are means ± SD of triplicate data (**p < 0.01, ***p < 0.001).
그림 Figure 6. Inhibition rate of extract from TK on elastase. Inhibition rate of elastase for TK extract and arbutin were determined with 2.5 U/mL elastase solution. ▮TK: Ternstroemia kwangtungensis Merr. ▯Ursolic acid: Ursolic acid. Result are means ± SD of triplicate data (**p < 0.01, ***p < 0.001).
그림 Figure 5. Inhibition rate of extract from TK on tyrosinase. Inhibition rate of tyrosinase for TK extract and arbutin were determined with 200 U/mL mushroom tyrosinase solution. ▮TK: Ternstroemia kwangtungensis Merr. ▯Arbutin: Arbutin. Result are means ± SD of triplicate data (**p < 0.01, ***p < 0.001).

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