[논문]Inhibitory effects of ultraviolet-C light and thermal treatment on four fungi isolated from pig slaughterhouses in Korea

Lee, Eun-Seon; Kim, Jong-Hui; Kang, Sun Moon; Kim, Bu-Min; Oh, Mi-Hwa

doi:10.5187/jast.2022.e17

Inhibitory effects of ultraviolet-C light and thermal treatment on four fungi isolated from pig slaughterhouses in Korea 원문보기

Journal of animal science and technology : JAST, v.64 no.2, 2022년, pp.343 - 352

Lee, Eun-Seon (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) , Kim, Jong-Hui (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) , Kang, Sun Moon (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) , Kim, Bu-Min (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) , Oh, Mi-Hwa (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration)

Abstract ▼ AI-Helper

Pig slaughterhouses harbor high humidity because of the necessary cleaning that takes place simultaneously with slaughter, which facilitates the existence of mold. Due to the enclosed space, there are several limitations to the control of mold growth with respect to cleaning, ventilation, and drying. In this study, the prevalence of fungi was investigated in four pig slaughterhouses in Korea. Four fungi (Aspergillus niger, Penicillium commune, Penicillium oxalicum, and Cladosporium cladosporioides) were detected with the highest frequency. These four strains were subjected to various treatments to reduce their growth. The fungi were inoculated onto stainless steel (SS) chips and treated with ultraviolet (UV)-C irradiation and hot water. Individual treatments with UV-C (15, 30, 90, 150, 300, and 600 mJ/cm2), and hot water (60, 65, 70, and 83℃) were performed to sanitize the SS chips. Simultaneous cleaning with 60℃ hot water and more than 150 mJ/cm2 of UV-C reduced the fungal incidence by > 6.5 Log from 6.6-7.0 Log CFU/cm2 (initial count). Our results demonstrate that a combined treatment of UV-C and hot water is the most economical and convenient way to prevent microbiological contamination of small tools (such as knives and sharpeners) and steel surfaces in slaughterhouses.

주제어

표/그림 (4)

그림 Fig. 1. Fungal occurrence in pig slaughterhouses. (A) Prevalence of fungi in the whole slaughterhouse according to fungal genera, and (B) distribution according to the processing step. ND, nipple drinker; L, lairage area; S, scale; SG, stunning gun; RS, rack after stunning, SH, shackle; CB, container for exsanguination; C, cleaning; SC, scalding; DH, dehairing; RD, rack after dehairing; SEC, second cleaning; SAW, saw; ET, evisceration tray; FC, final cleaning; ETA, evisceration table; CV, conveyer belt; H, hook; WC, wall of chilling room.
그림 Fig. 2. Inactivation of Aspergillus niger, Penicillium commune, Penicillium oxalicum, and Cladosporium cladosporioides on stainless steel (SS) chips after treatment with ultraviolet (UV)-C.
표 Table 1. The inhibitory effect of single thermal treatment on the SS chips
그림 Fig. 3. Inactivation of (A) Aspergillus niger, (B) Penicillium commune, and (C) Penicillium oxalicum on stainless steel chips after combined treatment with ultraviolet (UV)-C and hot water (60℃).

AI 본문요약
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제안 방법

To ensure a constant UV intensity output, the UV lamp was kept on for at least 30 min before each experiment, and the distance between the sample and lamp was maintained at 30 cm. For the combined treatment, hot water (60℃) treatment was performed immediately after UV-C irradiation (15, 30, 90, 150, and 300 mJ/cm2 ). Each step was conducted for the same time as that used for the single treatment described above.
In the hot water (60℃, 65℃, 70℃, and 83℃) treatments, the SS chips inoculated with each mold were immersed in hot water for 0.5, 1, 3, 5, and 10 min. The UV-C irradiation experiments were conducted using a bench scale UV chamber, HD 11077-4002A (Donghwa Plant, Osan, Korea) with a 15 W low-pressure UV-C lamp (Sankyo Denki, Kanagawa, Japan) that emitted monochromatic UV-C irradiation at 260 nm.
5, 1, 3, 5, and 10 min. The UV-C irradiation experiments were conducted using a bench scale UV chamber, HD 11077-4002A (Donghwa Plant, Osan, Korea) with a 15 W low-pressure UV-C lamp (Sankyo Denki, Kanagawa, Japan) that emitted monochromatic UV-C irradiation at 260 nm. The dosage of UV light exposure (mJ/cm2 ) was confirmed using a UV-C light meter, UVC-254A (Lutron, Taiwan).

대상 데이터

The suspension was boiled at 100℃ for 2 min and stored at −70℃ after cooling. Samples were sent to SolGent (Daejeon, Korea) to complete DNA sequencing. Internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA were amplified with two universal primers: ITS1 (5'-GTA GGT GAA CCT GCG G-3'), and ITS4 (5'-TCC GCT TAT TGA TAT GC-3').

데이터처리

Values of p < 0.05 were considered significant, and data were grouped using Duncan’s multiple range test.

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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