[논문]괭생이모자반 추출물의 리파아제 저해 활성 및 3T3-L1 지방전구세포 분화억제 효과

홍지우; 박하영; 박재현; 김소희; 김한아; 김진우

doi:10.4217/opr.2022006

괭생이모자반 추출물의 리파아제 저해 활성 및 3T3-L1 지방전구세포 분화억제 효과
Inhibition of Lipase Activity and Preadipocyte Differentiation in 3T3-L1 Cells Treated with Sargassum horneri Extract 원문보기

Ocean and polar research, v.44 no.1, 2022년, pp.61 - 67

홍지우 (선문대학교 건강보건대학 식품과학과) , 박하영 (선문대학교 건강보건대학 식품과학과) , 박재현 (선문대학교 건강보건대학 식품과학과) , 김소희 (선문대학교 건강보건대학 식품과학과) , 김한아 (선문대학교 건강보건대학 식품과학과) , 김진우 (선문대학교 건강보건대학 식품과학과)

Abstract ▼ AI-Helper

In this study, in order to evaluate the anti-obesity effect of sargassum horneri extract, the effects of the extract on lipase activity and preadipocyte differentiation in 3T3-L1 cells were investigated. S. horneri extract between 0.0 and 1.0 mg/mL showed no cytotoxicity and inhibited lipase activity by 68.1%. When S. horneri extract was utilized at levels of 0.25, 0.5, and 1.0 mg/mL in 3T3-L1 cells, preadipocytes differentiation decreased by 11.4, 19.7, and 25.6%, respectively, showing anti-obesity effects. In addition, after treatment with 1.0 mg/mL S. horneri extract, the mRNA expression levels of sterol regulatory element binding proteins-1c (SREBP-1c), peroxisome proliferator activated receptor-γ (PPAR-γ), CCAAT enhancer binding protein-α (CEBP-α), fatty acid synthase (FAS), and stearoyl-CoA desaturase1 (SCD1) in 3T3-L1 cells were significantly decreased (p < 0.05) by 65.2, 54.9, 50.0, 33.8, and 33.8% respectively. These results showed that S. horneri extract suppresses lipase activity and prophylactic preadipocyte differentiation in 3T3-L1, and thus can be used as an anti-obesity agent in functional foods and medicines.

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표/그림 (4)

표 Table 1. Primer sequences used in reverse transcription-polymerase chain reaction (RT-PCR) of major genes related to obesity
그림 Fig. 1. Effect of S. horneri extracts (0.0~4.0 mg/mL) on the cell viability in 3T3-L1 preadipocyte. The values were calculated as a percentage compared to the control group where the extract was not treated. Bars represent the mean±standard deviation of three independent experiments and Bars marked with asterisks are significantly different from the control ( *p < 0.05)
그림 Fig. 2. Inhibitory effect of S. horneri extract on preadipocyte differentiation and lipid accumulation of 3T3-L1 preadipocyte. Preadipocyte differentiation was initiated with MDI (0.5 mM IBMX, 1 mM DEX, and 1 μg/mL insulin) in 3T3-L1 treated with S. horneri extract. After 8 days, lipid droplets in adipocyte were fixed and stained with Oil red O (original magnification 64.0X). Bars marked with asterisks are significantly different from the control (*p < 0.05)
그림 Fig. 3. Effect of S. horneri extract on SREBP-1c, CEBP-α, PPAR-γ, FAS, and SCD1 mRNA expression levels in 3T3-L1. The statistical analysis of the data was conducted by a t-test. Values are expressed as mean±standard deviation of three experiments. Bars marked with asterisks are significantly different from the control (*p < 0.05)

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괭생이모자반 추출물의 리파아제 저해 활성 및 3T3-L1 지방전구세포 분화억제 효과
Inhibition of Lipase Activity and Preadipocyte Differentiation in 3T3-L1 Cells Treated with Sargassum horneri Extract 원문보기

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괭생이모자반 추출물의 리파아제 저해 활성 및 3T3-L1 지방전구세포 분화억제 효과 Inhibition of Lipase Activity and Preadipocyte Differentiation in 3T3-L1 Cells Treated with Sargassum horneri Extract 원문보기

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괭생이모자반 추출물의 리파아제 저해 활성 및 3T3-L1 지방전구세포 분화억제 효과
Inhibition of Lipase Activity and Preadipocyte Differentiation in 3T3-L1 Cells Treated with Sargassum horneri Extract 원문보기

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