[논문]종자 수분함량에 따른 개갑 인삼 종자의 저장성 연구

서수정; 유진; 장인배; 김영창

doi:10.7732/kjpr.2022.35.2.183

종자 수분함량에 따른 개갑 인삼 종자의 저장성 연구
Effect of Seed Moisture Content on Seed Storage of Dehisced Ginseng Seeds 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.35 no.2, 2022년, pp.183 - 191

서수정 (농촌진흥청 국립원예특작과학원 인삼특작부) , 유진 (농촌진흥청 국립원예특작과학원 인삼특작부) , 장인배 (농촌진흥청 국립원예특작과학원 인삼특작부) , 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부)

초록
AI-Helper

인삼은 다년생 식물로 종자 번식을 하는데, 수확 시 미성숙한 배(embryo)를 가지고 있어 후숙이 필요하다. 기후변화에 대비하고 안정적인 종자 보급을 위해서는 단기 및 장기간 대량으로 종자를 저장할 수 있는 기술이 필요하다. 본 연구에서는 2개 지역에서 얻은 개갑된 인삼 종자를 수분함량을 달리하여 -3.5℃에 보관하고, 저장 3, 15, 27개월 후에 유근과 지상부 출현을 관찰하여 발아력 검정을 통해 종자 저장성을 조사하였다. 3개월 후, 전반적으로 batch #1보다 batch #2에서 발아율이 높았고, 그리고 수분함량이 35% 이상에서만 발아가 원활하였다. 15개월 후, batch #2에서 수분함량이 45~54%인 부분 건조된 종자들에서 가장 높은 발아율을 보였고, 대부분의 무건조 종자는 부패하며 발아에 실패하였다. 수분함량 25% 이하로 건조된 종자들 역시 유근발아 또는 출아가 불량하였다. 부분 건조 종자들은 토양에서의 발아 및 생육도 우수하였지만, 1년 뒤에는 역시 활력을 유지하지 못하였다. 요약하면, 인삼 종자는 온대성 난저장 종자의 특성을 가지는 것으로 보이며, 부분 건조 조건에서는 종자의 수명을 연장할 수 있어 1년 정도의 단기 저장에 활용할 수 있을 것으로 판단된다.

Abstract ▼ AI-Helper

Ginseng (Panax ginseng C.A. Meyer) is a perennial plant and propagates by seeds, and those need after-ripening for germination. To be ready for climate change and to ensure a stable seed supply, the technique for storing seeds in short-term and long-term in large quantities is required. In this study, dehisced ginseng seeds from two locations, batch #1 and batch #2, were stored at -3.5℃ with different moisture content, and after 3, 15, and 27 months of storage, the percentage of radicle emergence and shoot emergence were measured. After 3 months, radicle emergence and shoot emergence were normal only when the seed moisture content was more than 35%, and overall, germination was higher in batch #2 than in batch #1. After 15 months, the partially dehydrated seeds, with a moisture content between 45 to 54%, showed the highest germination rates, and most of the undried seeds were spoiled and failed to germinate. Seeds with moisture content lower than 25% had poor germination, too. The partially dehydrated seeds also succeeded in germination and growth in the soil after 15 months of storage, but deteriorated after one more 1 year, too. In summary, ginseng seeds look like have temperate recalcitrant seed characteristics, and partial dehydration allows extension of seed longevity.

주제어

표/그림 (6)

그림 Fig. 1. Ginseng seeds. (A) Undehisced seeds after pulp remove, (B) dehisced seeds, and (C) germinating seeds.
표 Table 1. Seed moisture contents measured after seed dry at each DMC
표 Table 2. Germination properties of batch #1 and batch #2 ginseng seeds after 3 month of cold-storage
표 Table 3. Germination properties of batch #1 and batch #2 seeds after 15 month of cold-storage
그림 Fig. 2. Rate of shoot emergence and damping-off of ginseng grown from each dried seed of batch #2 in nursery soil. Bars represent the means ± standard error of the triplicate experiments. Different letters on the bar are significantly different by Duncan's Multiple Range Test (DMRT, p < 0.05).
표 Table 4. Germination properties of batch #1 and batch #2 seeds after 27 month of cold-storage

참고문헌 (30)

Baskin, C.C. and J.M. Baskin. 2014. Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination, 2nd ed. Academic Press, CA (USA). pp. 119-143.
Bonner, F.T. 1990. Storage of seeds: Potential and limitations for germplasm conservation. Forest Ecol. Manag. 35:35-43.
Choi, C.H., W.S. Tak and K.J. Cho. 2006. Effect of priming treatment using PEG on seed germination in Acer mono Max. Korean J. Plant Res. 19:480-487 (in Korean).
Connor, K.F. and F.T. Bonner. 1999. Effects of Temperature and Moisture Content on the Storability of Hardwoods Seeds: In Haywood, J.D. (ed.), Tenth Biennial Southern Silvicultural Research Conference. USDA Forest Service, Southern Research Station. MS (USA). pp. 123-126.
Derkx, M.P.M. 2000. Pre-treatment at controlled seed moisture content as an effective means to break dormancy in tree seeds: In Vimont, J.D. and J. Grabbe (eds), Dormancy in Plants: From whole plant behaviour to cellular control. CABI, Wallingford, UK. pp. 68-85.
Ellis, R.H., T.F. Hong and E.G. Roberts. 1990. An intermediate category of seed storage behaviour? I. Coffee. J. Exp. Bot. 41:1167-1174.

상세보기
Gosling, P.G., Y. Samuel and A. Peace. 2003. The effect of moisture content and prechill duration on dormancy breakage of Douglas fir seeds (Pseudotsuga menziesii var. menziesii [Mirb.] Franco). Seed Sci. Res. 13:239-246.

상세보기
Ha, Y.I., J.M. Lim, S.M. Ko, J.R. Liu and D.W. Choi. 2006. Sequence variability and expression characteristics of the ginseng (Panax ginseng C.A. Meyer) dehydrin gene family. J. Plant Biol. 49:205-211.
Han, E.J., E. Popova, G.T. Cho, S.U. Park, S.C. Lee, H.W. Pritchard and H.H. Kim. 2016. Post-harvest embryo development in ginseng seeds increases desiccation sensitivity and narrows the hydration window for cryopreservation. Cryo-Lett. 37:284-294.
Jones, S.K. and P.G. Gosling. 1994. "Target moisture content" prechill overcomes the dormancy of temperate conifer seeds. New Forest 8:309-321.
Kim, D.H., S.H. Han, J.H. Song and K.G. Jang. 2012. Seed storage and longevity in woody plant. Korea Forest Research Institute, Seoul, Korea. pp. 49-118 (in Korean).
Kim, H.H., J.H. Lee, D.J. Shin, H.C. Ko, H.S. Hwang, T. Kim, E.G. Cho and F. Engelmann. 2008. Desiccation sensitivity and cryopreservation of Korean ginseng seeds. Cryo-Lett. 229:419-426.
Kim, Y.C., Y.B. Kim, I.H. Jo, J.U. Kim, J.W. Lee, K.H. Bang, D.H. Kim and K.H. Kim. 2015a. Stratification characteristics of ginseng seed by storage period (Panax ginseng C.A. Meyer). Proceedings of Symposium. May 2015. Korean J. Medicinal Crop. Sci. pp. 119-120 (in Korean).
Kim, Y.C., Y.B. Kim, J.U. Kim, J.W. Lee, I.H. Jo, K.H. Bang, D.H. Kim and K.H. Kim. 2015b. Difference in growth characteristics of 5-year-old ginseng grown by direct seeding and transplanting. Korean J. Medicinal Crop. Sci. 23:480-488 (in Korean).
Kleinwachter, M., A. Radwan, M. Hara and D. Selmar. 2014. Dehydrin expression in seeds: an issue of maturation drying. Front. Plant Sci. 5:402.
Kwon, W.S., C.M. Jung, S.D. Ahn and K.T. Choi. 1986. Effects of growth regulators on the germination of Panax ginseng C.A. Meyer. J. Ginseng Res. 10:159-166 (in Korean).
Kwon, W.S., J.H. Lee and M.G. Lee. 2001. Optimum chilling terms for germination of the dehisced ginseng (Panax ginseng C.A. Meyer) seed. J. Ginseng Res. 25:167-170 (in Korean).
Lee, E.K., C.H. Bae, Y.J. Kim, S.D. Park, J.J. Shim, Y. Yu and J.L. Lee. 2021. Bioavailability and anti-inflammatory effect of fermented red ginseng in BALB/c mouse. Korean J. Plant Res. 34:433-442 (in Korean).
Lee, J.C. and J.T.A. Proctor. 1996. Low temperature tolerance of Panax quinquefolium. J. Ginseng Res. 20:179-183.
Lee, J.C., J.S. Byen and J.T.A. Proctor. 1986. Dormancy of ginseng seed as influenced by temperature and gibberellic acids. Korean. J. Crop Sci. 31:220-225.
Lee, J.H., S.S. Lee, I.O. Ahn, J.Y. Kang and M.G. Lee. 2004. Relationship between storage periods and germination ability of dehisced seeds of Panax ginseng C.A. Meyer. J. Ginseng Res. 28:215-218 (in Korean).
Lee, J.W., Y.C. Kim, J.U. Kim, I.H. Jo, H.K. Kim and D.H. Kim. 2016. Effects of gibberellic acid and alternating temperature on breaking seed dormancy of Panax ginseng C.A. Meyer. Korean J. Medicinal Crop Sci. 24:284-293 (in Korean).
Noland, T.L., A.E. Morneault, D.C. Dey and D. Deugo. 2013. The effect of storage temperature and duration on northern red oak acorn viability and vigour. Forest. Chron. 89:769-776.
O'Reilly, C. and N. de Atrip. 2007. Seed moisture content during chilling and heat stress effects after chilling on the germination of common alder and downy birch seeds. Silva Fenn. 41:235-246.
Park, H.B., C.H. Ko, S.Y. Kim, K.C. Lee, J.H. Kim and J.M. Chung. 2019. Dormancy type and germination characteristics of seeds of Lonicera chrytsantha Turcz. Ex Ledeb (Caprifoliaceae). Korean J. Plant Res. 32:457-462 (in Korean).
Rajametov, S., Y.Y. Lee, Y.C. Kim, S. Lee, J.Y. Yi, Y.A. Jeon, J.S. Sung, G.A. Lee and J.G. Gwak. 2014. Response of pre and post treatments for cryopreservation of Korean ginseng seeds on recovering viability. Korean J. Breeding Sci. 46: 408-416.
RDA (Rural Development Administration). 2014. Ginseng. RDA. Jeonju, Korea.
Suh, S.J., I.B. Jang, I.B. Jang, J.W. Moon and J. Yu. 2019. Optimum storage temperature for spring sowing of Panax ginseng seeds. Korean J. Medicinal Crop Sci. 27:390-396 (in Korean).
Suh, S.J., I.B. Jang, J. Yu, I.B. Jang, H.W. Park, T.S. Seo and K.B. Kweon. 2017. Effect of seed dehydration and temperature during cold-stratification on the seed quality of Panax ginseng C.A. Meyer. Korean J. Medicinal Crop Sci. 25:209-216 (in Korean).
Yoon, J.W., H.H. Kim, J.H. Lee, J.K. Choi, S.S. Lee, Y.M. Choi and T.S. Kim. 2005. Optimal desiccation condition and moisture content of dehisced seeds of ginseng (Panax ginseng C.A. Meyer) for cryopreservation. Korean J. Crop Sci. 50:406-410 (in Korean).

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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