[논문]함유황 다당체 Fucoidan의 인체 대장암세포(HT-29) 사멸과 Apoptosis에 미치는 영향

김민지; 정하숙

doi:10.9799/ksfan.2022.35.3.204

함유황 다당체 Fucoidan의 인체 대장암세포(HT-29) 사멸과 Apoptosis에 미치는 영향
Effects of Fucoidan, a Sulfur-Containing Polysaccharide, on Cytotoxicity and Apoptosis in HT-29 Human Colorectal Cancer Cells 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.35 no.3, 2022년, pp.204 - 212

김민지 (덕성여자대학교 과학기술대학 식품영양학과) , 정하숙 (덕성여자대학교 과학기술대학 식품영양학과)

Abstract ▼ AI-Helper

The purpose of this study was to investigate the biological activity of fucoidan, a sulfur-containing polysaccharide, on cytotoxicity and apoptosis in the human HT-29 colorectal cancer cell line using cell viability, Flow cytometry, Western blot, and RT-PCR analyses. Fucoidan inhibited the proliferation of HT-29 cells by 39.6% at a concentration of 100 ㎍/mL for 72 h. The inhibition was dose-dependent and accompanied by apoptosis. Flow cytometric analysis showed that fucoidan increased early apoptosis and late apoptosis by 65.84% and 72.09% at concentrations of 25 and 100 ㎍/mL, respectively. Analysis of the mechanism of these events indicated that fucoidan-treated cells exhibited increases in the activation of caspase-3, caspase-8, and PARP in a dose-dependent manner. These results suggest that fucoidan may inhibit the growth of human colorectal cancer cells by various apoptosis-promoting effects, as well as by apoptosis itself.

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표/그림 (8)

그림 Fig. 1. Cytotoxic effects of fucoidan in HT-29 cells in time and dose dependent manners. After treatment with fucoidan for 24, 48, and 72 hr, the cell viability was investigated as described in the Materials and Methods. The values are mean±S.D. of three independent experiments. Bars with different alphabet letters are significantly different at p<0.05.
그림 Fig. 2. Effects of fucoidan on apoptosis in HT-29 cells in a dose dependent manner. The right bottom quadrant represents Annexin V-stained cells (early-phase apoptotic cells). The top right quadrant represents PI- and Annexin V-stained cells (late-phase apoptotic cells). A: 0 μg/mL, B: 25 μg/mL, C: 50 μg/mL, D: 100 μg/mL of fucoidan.
표 Table 1. The population of early-apoptosis (Annexin V-FITC positive, PI negative) and late apoptosis (Annexin V-FITC positive, PI positive) treated with fucoidan in HT-29 cells for 72 hr
그림 Fig. 3. Effects of fucoidan on the expression of caspase-related proteins in HT-29 cells in a dose dependent manner. Cells were treated with 0, 25, 50 and 100 μg/mL of fucoidan for 72 hr. The cell lysates were electrophoresed and Western blotting with caspase-8, caspase-3, and PARP antibodies.
그림 Fig. 4. Effects of fucoidan on the expression of Bcl-2 family related proteins in HT-29 cells in a dose dependent manner. Cells were treated with 0, 25, 50 and 100 μg/mL of fucoidan for 72 hr.
그림 Fig. 5. Effects of fucoidan on Bcl-2/Bax ratio(%) in HT-29 cells. Quantitative analysis of band was estimated by densitometric analysis. The values are mean±S.D. of three independent experiments. Bars with different alphabet letters are significantly different at p<0.05.
그림 Fig. 6. Effects of fucoidan on Bax mRNA expression in HT-29 cells. Quantitative analysis of band was estimated by densitometric analysis. The values are mean±SD of three independent experiments. Bars with different alphabet letters are significantly different at p<0.05.
그림 Fig. 7. Effects of fucoidan on p53 mRNA expression in HT-29 cells. Quantitative analysis of the band was estimated by densitometric analysis. The values are mean± SD of three independent experiments. Bars with different alphabet letters are significantly different at p<0.05.

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