[논문]동백 꽃 아임계 수 추출물의 항염 및 항산화 활성

김정은; 고예린; 부석환; 강성희; 이남호

doi:10.15230/scsk.2022.48.2.97

동백 꽃 아임계 수 추출물의 항염 및 항산화 활성
Anti-inflammatory and Anti-oxidative Activities for the Subcritical Water Extract of Camellia japonica Flowers 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.48 no.2, 2022년, pp.97 - 104

김정은 (제주대학교 화학.코스메틱스학과) , 고예린 (동안(주)) , 부석환 (동안(주)) , 강성희 (동안(주)) , 이남호 (제주대학교 화학.코스메틱스학과)

초록
AI-Helper

본 연구에서는 동백 꽃 아임계 수 추출물(SWE, 135 ~ 180 ℃, 70 bar 조건)의 항염 및 항산화 효능을 70% 에탄올 및 열수 추출물과 비교 분석하였다. 이들 추출물 중 180 ℃, 70 bar의 조건으로 추출한 아임계수 추출물의 수율(57.9%)이 가장 높게 나타났으며, 이는 열수 추출물(28.1%)에 비해서 2배 이상 추출 수율이 증가하였다. Lipopolysaccharide (LPS)로 자극된 RAW 264.7 대식세포를 이용한 nitric oxide (NO) 생성 억제 활성 실험 결과, 아임계 수 추출물이 세포 독성 없이 NO의 생성을 저해시키는 효과가 70% 에탄올 및 열수 추출물보다 우수함을 확인하였다. 또한 DPPH 및 ABTS⁺ 라디칼 소거 활성 실험 결과, 아임계 수 추출물의 라디칼 소거 활성이 70% 에탄올 및 열수 추출물과 유사하게 나타났다. 동백 꽃의 주성분인 gallic acid의 함량을 HPLC를 이용하여 분석한 결과, 아임계 수 추출물(165 ℃, 70 bar)에서 함량이 1.62 mg/g으로 가장 높게 분석되었다. 이상의 연구결과를 바탕으로 동백 꽃 아임계 수 추출물은 항염 및 항산화 효과를 갖는 천연 화장품 소재로써 활용이 가능할 것이라 사료된다.

Abstract ▼ AI-Helper

In this study, the anti-inflammatory and anti-oxidant efficacy of camellia subcritical water extracts (SWE, 135 ~ 180 ℃, 70 bar) was compared with 70% ethanol and hot water extracts. Among these extracts, the yield (57.9%) of the subcritical water extract, which was extracted under the condition of 180 ℃ and 70 bar was the highest, which increased the extraction yield by more than two times compared to the hot water extract (28.1%). The results of the nitric oxide (NO) production inhibition activity experiment using RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS) showed that subcritical water extracts had superior effects in inhibiting the production of NO without cytotoxicity than 70% ethanol and hot water extracts. In addition, DPPH and ABTS+ radical scavenging activity experiments showed that the radical scavenging activity of subcritical water extract was similar to that of 70% ethanol and hot water extract. Moreover, the content of gallic acid was determined by HPLC and the quantity was about 1.62 mg/g for the SWE (165 ℃, 70 bar), which was the highest among all of the extracts. Based on these results, it is concluded the SWE of C. japonica flowers could be potentially applicable as anti-inflammatory and anti-oxidative ingredients in cosmetic formulations.

주제어

표/그림 (6)

그림 Figure 1. Effects of subcritical water extract (SWE), 70% EtOH and hot water extract from C. japonica flowers on NO production (A) and cell viability (B) in LPS-stimulated RAW 264.7 cells. The cells were stimulated with 100 ng/mL of LPS only, or with LPS plus various extract from C. japonica flowers for 24 h. The data represent the mean ± SD of triplicate experiments. (*p < 0.05, **p < 0.01)
표 Table 1. Extraction Condition and Yield of C. japonica Flowers Extract by Various Extraction Methods
그림 Figure 2. DPPH (A) and ABTS⁺ (B) radical scavenging activities of subcritical water extract (SWE), 70% EtOH and hot water extract from C. japonica flowers. The data are expressed as a percentage of control and represent the mean ± SD of triplicate experiments. (*p < 0.05, **p < 0.01)
표 Table 2. SC₅₀ Values of C. japonica Flowers Extract by Various Extraction Methods
그림 Figure 3. HPLC chromatogram of 70% EtOH (A), hot water (B), subcritical water extract (135 ℃ (C), 150 ℃ (D), 165 ℃ (E), 18 0 ℃ (F)) from C. japonica flowers.
표 Table 3. Content of Gallic Acid from C. japonica Flowers Extract by Various Extraction Methods

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