[논문]목향과 향부자 추출물이 흰쥐의 만성 스트레스에 미치는 실험적 효과

최찬헌; 홍준영; 정현우

doi:10.15188/kjopp.2022.04.36.2.54

[국내논문] 목향과 향부자 추출물이 흰쥐의 만성 스트레스에 미치는 실험적 효과
Experimental Effects of Aucklandiae Radix and Cyperi Rhizoma Extract on Chronic Stress in Rats 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.36 no.2, 2022년, pp.54 - 58

최찬헌 (동신대학교 한의과대학) , 홍준영 (동신대학교 한의과대학) , 정현우 (동신대학교 한의과대학)

Abstract ▼ AI-Helper

This study aimed to investigate the anti-stress function of Aucklandiae Radix (AR) and Cyperi Rhizoma (CR). The essential oils used in the experiment were extracted from AR and CR using Steam Distillation Extraction and Super critical CO2 extraction. To observe the effects of sample administration, we measured feed intake, leukocytes, red blood cells, hemoglobin, platelets, serum serotonin content, immobility time, climbing time, and swimming time in mice subjected to chronic restraint stress as behavioral changes. The average body weight of all experimental groups increased than the average body weight of the control group. The immobility and climbing times of experimental groups A and B administered with supercritical extraction samples were shorter than those of the other experimental groups and the control group, and the swimming time was longer. The serotonin content in the blood of all experimental groups decreased compared to the normal group, and the serotonin content of the control group was increased. The authors suggest that Korean herbal medicines AR and CR may be utilized as anti-stress flavoring agents based on the above results.

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표/그림 (12)

그림 Fig. 1. Chronic restraint stress model rats
그림 Fig. 2. Oil inhaled suction device
그림 Fig. 3. Forced swimming test experimental design
그림 Fig. 4. The diagram of experiment. FST: Forced swimming test
그림 Fig. 5 . Cytotoxicity of ARE and CRE in thymocytes. ARE was Aucklandiae Radix Extracts, CRE was Cyperi Rhizoma Extracts and ARCRE was Aucklandiae Radix and Cyperi Rhizoma Extracts. After attaching thymocytes to 96 well plate, each extracts was treated according to concentration. After 48 hrs incubation, the proliferation rate were measured using Microplate Reader 450 ㎚. Results are expressed as the mean and standard deviation.
그림 Fig. 6. Cytotoxicity of ARE and CRE in splenocytes. After attaching splenocytes to 96 well plate , each extracts was treated according to concentration. After 48 hrs incubation, The proliferation rate were measured using Microplate Reader 450 ㎚. Results are expressed as the mean and standard deviation. * : Statistically, significance compared with non-treated group(0 ㎎/㎖)(*; p<0.05) (n=8)
그림 Fig. 7. Weight change in the restraining stress model inhaled AR and CR oil flavor. Normal group without chronic restraint stress were sprayed saline, Control groups induced by chronic restraint stress were sprayed saline instead of oil. Under restraint stress, Sample A, B, C and D group were inhaled ARSCO (1%), CRSCO (1%), ARSDO (1%) and CRSDO (1%). Results are expressed as the mean and standard deviation.
그림 Fig. 8. The change in the average food intake of the restraining stress model inhaled by AR and CR oil flavor. Results are expressed as the mean and standard deviation. Other descriptions are the same as in Fig. 7.
그림 Fig. 9. Changes in serum WBC, RBC and Hemoglobin of the restraining stress model inhaled by AR and CR oil flavor. Results are expressed as the mean and standard deviation. Other legends are the same as in Fig. 7. * : Statistically, significance compared with Control group(*; p<0.05, ** ; p<0.01) (n=8)
그림 Fig. 10. Changes in serum Platlet of the restraining stress model inhaled by AR and CR oil flavor. Results are expressed as the mean and standard deviation. Other legends are the same as in Fig. 7. * : Statistically, significance compared with Control group(*; p<0.05) (n=8)
그림 Fig. 11. Behavioral Changes in Forced Swimming Tests of the restraining stress model inhaled by AR and CR oil flavor. Results are expressed as the mean and standard deviation. Other legends are the same as in Fig. 7.
그림 Fig. 12. Variation of Serotonin contents in serum of the restraining stress model inhaled by AR and CR oil flavor. Results are expressed as the mean and standard deviation. Other legends are the same as in Fig. 7.

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