[논문]오령산(五苓散)의 본태성 고혈압 동물모델에서 혈압강하 및 혈관 이완 효과

장윤재; 김혜윰; 홍미현; 윤정주; 이호섭; 강대길

doi:10.14374/hfs.2022.30.3.123

오령산(五苓散)의 본태성 고혈압 동물모델에서 혈압강하 및 혈관 이완 효과
Effect of Oryeongsan on Spontaneously Hypertensive Rat decrease of Blood Pressure and Vasodilatory 원문보기

大韓韓醫學方劑學會誌 = Herbal formula science, v.30 no.3, 2022년, pp.123 - 135

장윤재 (원광대학교 한의과대학 생리학교실) , 김혜윰 (원광대학교 한의과대학 생리학교실) , 홍미현 (원광대학교 한의과대학 생리학교실) , 윤정주 (원광대학교 한의과대학 생리학교실) , 이호섭 (원광대학교 한의과대학 생리학교실) , 강대길 (원광대학교 한의과대학 생리학교실)

Abstract ▼ AI-Helper

Oryeongsan (ORS), a formula composed of five herbal medicines, has long been used to treat impairments of the regulation of body fluid homeostasis. The purpose of this study was to determine the antihypertensive and renal protective effects of ORS in rats with hypertension. Spontaneously hypertensive rats (SHR) were divided into two groups with similar mean baseline systolic blood pressure (SBP). Then, 1 mL/kg of vehicle (distilled water) or 1.5, 3 g/kg of ORS extract were administered orally once a day for 4 weeks. SBP and diastolic blood pressure (DBP) were measured at weeks 1, 2, 3 and 4. At the end of the experiment, blood was collected, and heart were removed for histology. By the 2 weeks after initiation of treatment, the ORS treated group had significantly lower SBP than SHR rats. The ORS treatment significantly improved blood pressure and echocardiogram parameters compared to hypertensive rats. Additionally, the left ventricular (LV) remodeling and LV dysfunction were significantly improved in ORS treated group hypertensive rats. Furthermore, an increase in fibrotic area has been observed in SHR rats compared with Wistar-Kyoto rats (WKY). Furthermore, administration of ORS significantly attenuated cardiac fibrosis in hypertensive rats. Therefore, these findings suggest that ORS has a protective effect on heart failure by alleviating hypertensive heart disease and cardiovascular dysfunction in SHR.

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표/그림 (6)

그림 Fig. 1. Effects of ORS on blood pressure and heart rate in rats. Systolic blood pressure (A). Final-Systolic blood pressure (B). The values represent the means ± standard error (n = 5 for each group). ***p < 0.001 vs. WKY; ^###p < 0.001 vs. SHRs.
그림 Fig. 2. Effects of ORS on the endothelium-dependent relaxant effects of acetylcholine in phenylephrine contracted arteries from WKY or SHR. Concentration–response curves following sodium nitroprusside (SNP, A) and acetylcholin (Ach, B) treatments of WKY or SHRs aortic rings. This attenuated relaxation was sangdanghi improved by ORS-H treatment. The levels of plasma lactate dehydrogenase (LDH, C). The values represent the means ± standard error (n = 5 for each group). *p < 0.05, **p < 0.01, ***p < 0.001 vs. WKY; ^#p < 0.05, and ^##p < 0.005 vs. SHRs.
$Fig. 3. Effect of ORS decoction in the left ventricular function. Measurement has been taken by echocardiography. M-Mode echocardiogram images were represented from individual groups (A). The values of ejection fraction (EF, B) and fractional shortening (FS, C) were calculated by echocardiographic parameters. The values represent the means ± standard error (n = 5 for each group). ***p < 0.001 vs. WKY; <sup>#</sup>p < 0.05, and <sup>###</sup>p < 0.001 vs. SHRs.$ 그림 Fig. 3. Effect of ORS decoction in the left ventricular function. Measurement has been taken by echocardiography. M-Mode echocardiogram images were represented from individual groups (A). The values of ejection fraction (EF, B) and fractional shortening (FS, C) were calculated by echocardiographic parameters. The values represent the means ± standard error (n = 5 for each group). ***p < 0.001 vs. WKY; ^#p < 0.05, and ^###p < 0.001 vs. SHRs.
그림 Fig. 4. Effects of ORS on cardiac hypertrophy. Heart macroscopic images(A). heart weight (HW)/body weight (BW)(B) and Left ventricle (LV)/BW(C) ratios for the indicated groups. The values represent the means ± standard error (n = 5 for each group). ***p < 0.001 vs. WKY; ^#p < 0.05, ^##p < 0.005, and ^###p < 0.001 vs. SHRs.
그림 Fig. 5. Masson's trichrome staining in SHR hypertension rats (Magnification x40)(A). area(B) and fibrosis(C) in SHR hypertension rats. The values represent the means ± standard error (n = 3 for each group). ***p < 0.001 vs. WKY; ###p < 0.001 vs. SHRs.
그림 Fig. 6. Effect of ORS on histological changes in cardiac fibrosis. The detailed anatomy of the changes in Masson’s trichrome staining (A,B). The values represent the means ± standard error (n = 3 for each group). ***p < 0.001 vs. WKY; ^###p < 0.001 vs. SHRs.

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