[논문]LPS로 유도된 마우스 대식세포주인 RAW264.7에서 MAPK 조절에 의한 백미 물추출물의 항염증 활성

이상호; 유지현; 길기정

doi:10.6116/kjh.2022.37.6.19.

[국내논문] LPS로 유도된 마우스 대식세포주인 RAW264.7에서 MAPK 조절에 의한 백미 물추출물의 항염증 활성
Anti-inflammatory Activity of Cynanchi Atrati Radix Et Rhizoma Water Extracts via Regulation of MAPK in LPS-induced Murine Macrophage Cell Line, RAW 264.7 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.6, 2022년, pp.19 - 28

이상호 ((주)뉴트렉스테크놀러지) , 유지현 (중부대학교 한방보건제약학과) , 길기정 (중부대학교 한방보건제약학과)

Abstract ▼ AI-Helper

Objectives : To develop natural ingredients that help prevent or treat anti-inflammatory-related diseases and use themas basic data, we investigated anti-inflammatory activity of Cynanchi Atrati Radix Et Rhizoma water extracts(CWE) in lipopolysaccharide(LPS)-induced murine macrophage cell line, RAW 264.7 cells. Methods : The cell viabilities were evaluated with RAW 264.7 cells. The production of nitric oxide(NO), prostaglandin E2(PGE2), pro-inflammatory cytokines such tumor necrotic factor(TNF)-α and interleukin(IL)-6 were assessed in LPS-induced RAW 264.7 cell treated with CWE. Furthermore, the protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2), and mitogen-activated protein kinase(MAPK) were assessed by western blotting. Results : In RAW 264.7 cell, the cell viability by CWE treatment was more than 98.4% at a concentration of 100-400 ㎍/mL. At a concentration of 800 ug/ml of CWE, the cell viability was as low as 86%. At doses of 100, 200 and 400 ㎍/mL, CWE inhibited the production of NO, PGE2, TNF-𝛼 and IL-6 in a dose-dependent manner and also decreased the expression of iNOS and COX-2 from LPS-induced RAW 264.7 cells. In addition, CWE significantly inhibited the MAPK pathway including decreased the phosphorylation of the p38, c-Jun N-terminal kinase(JNK) and extracellular signal-regulated kinase(ERK1/2). Conclusions : Our study provides evidence that CWE inhibits the production of main pro-inflammatory molecules in LPS-induced RAW 264.7 cells via expression of p38, JNK, and ERK1/2 MAPK signaling pathways. Therefore, CWE is expected to be widely used as a natural ingredient for anti-inflammatory functional foods or pharmaceuticals in the future.

Keyword

표/그림 (6)

그림 Fig. 1. Effect of CWE on cell viability in murine macrophage cell line, RAW 264.7 cell. Cell viabilities were measured with CCK-8 assay. RAW 264.7 cells were pre-treated with various concentrations of CWE for 1 h, and then LPS(1 ㎍/mL) was treated for 24 h. The concentration of CWE 100, 200, 400 and 800 ㎍ /mL. All values are means±standard deviations of triplicate determinations. Means with different letters(a-b) indicate significant difference(p<0.05) by Duncan's multiple range test.
그림 Fig. 2. Effect of CWE on NO in LPS-induced RAW 264.7 macrophage cells. RAW 264.7 cells were pre-treated with various concentrations of CWE for 1 h, and then LPS(1 ㎍/mL) was treated for 24 h. The concentration of CWE was 100, 200 and 400 ㎍/mL. All values are means±standard deviations of triplicate determinations. Means with different letters(a-e) indicate significant difference(p<0.05) by Duncan's multiple range test.
그림 Fig. 3. Effect of CWE on NO in LPS-induced murine macrophage cell line, RAW 264.7 cell. RAW 264.7 cells were pre-treated with various concentrations of CWE for 1 h, and then LPS(1 ㎍/mL) was treated for 24 h. The concentration of CWE was 100, 200 and 400 ㎍/mL. All values are means±standard deviations of triplicate determinations. Means with different letters(a-e) indicate significant difference(p<0.05) by Duncan's multiple range test.
그림 Fig. 4. Effects of CWE on TNF-α and IL-6 production in LPS-induced murine macrophage cell line, RAW 264.7 cell. RAW 264.7 cells were pre-treated with various concentrations of CWE for 1 h, and then LPS(1 ㎍/mL) was treated for 24 h. The concentration of CWE 100, 200 and 400 ㎍/mL. All values are means±standard deviations of triplicate determinations. Means with different letters(a-e) indicate significant difference(p<0.05) by Duncan's multiple range test.
그림 Fig. 5. Effect of CWE on iNOS and COX-2 protein expression in LPS-induced murine macrophage cell line, RAW 264.7 cell. RAW 264.7 cells were pre-treated with various concentrations of CWE for 1 h, and then LPS(1 ㎍/mL) was treated for 24 h. The concentration of CWE was 100, 200 and 400 ㎍/mL. β-actin was used as an internal control. All values are means±standard deviations of triplicate determinations. Means with different letters(a-d) indicate significant difference(p<0.05) by Duncan's multiple range test.
그림 Fig. 6. Effect of CWE on MAPK singnal expression in LPS-induced murine macrophage cell line, RAW 264.7 cell. RAW 264.7 cells were co-incubated with the indicated concentrations(100, 200 and 400 ㎍/mL) of CWE and LPS for 30 min and whole-cell lysates were analysed by western blotting using antibodies against p38, JNK, and ERK(1/2), and the respective phosphorylated forms. All values are means±standard deviations of triplicate determinations. Means with different letters(a-e) indicate significant difference(p<0.05) by Duncan's multiple range test.

참고문헌 (48)

National Institute of Biological Resources. National Institute of Biological Information Management System. 2016.
Park JH. Encyclopedia of Herbal Medicine.(volume one). Seoul : Shinilbooks. 2002 ; 312.
Park MG. Park JU, Yang SW, Kim JJ. Preservative composition comprising Cynanchum atratum Bunge extract as active ingredient and method of manufacturing the same. Seoul : AJ Co., Ltd. 2017 ; 10-2017-0029518.
Yuan Y, Zhang WD, Zhang C, Liu RH and Jin HZ. Studies on chemical constituents from root of Cynanchum atratum. China J. Chin. Mater. Med. 2007 ; 32 : 1895-8.
Jin Q, Han XH, Yun CY, Lee C, Lee JW, Lee DH, Lee MY, Jung SH, Hong JT, Kim YS and Hwang BY. Melanogenesis inhibitory pregnane glycosides from Cynanchum atratum. Bioorganic & Medicinal Chemistry Letters. 2018 ; 28 : 1252-6.

상세보기
Lee KY, Sung SH, and Kim YC. New acetylcholinesterase-inhibitory pregnane glycosides of Cynanchum atratum Roots. Helvetica Chimica Acta. 2003 ; 86 : 474-83.

상세보기
Yang J, Wang B, Zhang C, Xu X and Zhang M. A C21-steroidal glycoside from Cynanchum atratumattenuates Concanavalin A-induced liver injury in Mice. Molecules. 2019 ; 24 : 1-13.

상세보기
Lee KY, Yoon JS, Kim ES, Kang SY and Kim YC. Anti-acetylcholinesterase and anti-amnesic activities of a pregnane glycoside, cynatroside B, from Cynanchum atratum. Planta Medica. 2005 ; 71 : 7-11.

상세보기
Hu G, Hong D, Zhang T, Duan H, Wei P, Guo X and Xu X. Cynatratoside-C from Cynanchum atratumdisplays anti-inflammatory effect via suppressing TLR4 mediated NF-κB and MAPK signaling pathways in LPS-induced mastitis in mice. Chemico-Biological Interactions. 2018 ; 279 : 187-95.

상세보기
Mukudai Y, Kondo S, Koyama T, Li C, Banka S, Kogure A and Shintani S. Potential anti-osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro. BMC Complementary and Alternative Medicine. 2014 ; 29 : 1-14.
Yan Y, Zhang J, Liu K, Huang T, Yan C, Huang L and Hao X. Seco-pregnane steroidal glycosides from the roots of Cynanchum atratum and their anti-TMV activity. Fitoterapia. 2014 ; 97 : 50-63.

상세보기
Kim YY, Lee S, Jang HJ, Hur G, Lee SW, Jung KS, Lee SJ, KIM SH and Rho MC. Cynanchum atratum ameliorates airway inflammation via maintaining alveolar barrier and regulating mast cell-mediated inflammatory responses. The American Journal of Chinese Medicine. 2019 ; 47 : 1795-814.

상세보기
Comalada M, Lloberas J and Celada A. MKP-1: a critical phosphatase in the biology of macrophages controlling the switch between proliferation and activation. Eur J Immunol. 2012 ; 8 : 1938-48.

상세보기
Lu Y, Suh SJ, Kwak CH, Kwon KM, Seo CS, Li Y, Jin Y, Li Xian, Hwang SL, Kwon O, Chang YC, Park YG, Park SS, Son JK, Kim CH and Chang HW. Saucerneol F, a new lignan, inhibits iNOS expression via MAPKs, NF-kappaB and AP-1 inactivation in LPS-induced RAW264.7 cells. Int. Immunopharmacol. 2012 ; 12 : 175-81.

상세보기
Cheng BCY, Ma XO, Kwan HY, Tse KW, Cao HH, Su T, Shu X, Wu ZZ and Yu ZL. A herbal formula consisting of rosae multiflorae fructus and lonicerae japonicae flos inhibits inflammatory mediators in LPS-stimulated RAW 264.7 macrophages. J. Ethnopharmacol. 2014 ; 153 : 922-7.

상세보기
Nyati KK, Masuda K, Zaman MMU, Dubey PK, Millrine D, Chalise JP, Higa M, Li S, Standley DM, Saito K, Hanieh H and Kishimoto T. TLR4-induced NF-κB and MAPK signaling regulate the IL-6 mRNA stabilizing protein Arid5a. Nucleic Acids. Res. 2017 ; 45 : 2687-703.

상세보기
Chan ED and Riches DW. IFN-γ+ LPS induction of iNOS is modulated by ERK, JNK/SAPK, and p38mapk in a mouse macrophage cell line. Am. J. Physiol. Cell. Physiol. 2001 ; 280 : C441-50.

상세보기
Lee YH and Lim EM. Anti-Inflammatory effect of Ligustri lucidi fructus water extract in RAW 264.7 cells induced by LPS. J Korean Obstet Gynecol. 2013 ; 26 : 66-81.
Li J, Zhang H, Huang W, Qian H and Li Y. TNF-alpha inhibitors with anti-oxidative stress activity from natural products. Curr. Top Med. Chem. 2012 ; 12 : 1408-21.
Saw CL, Wu Q, Su ZY, Wang H, Yang Y and Xu X. Effects of natural phytochemicals in Angelica sinensis(Danggui) on Nrf2-mediated gene expression of phase II drug metabolizing enzymes and anti- inflammation. Biopharm. Drug Dispos. 2013 ; 34 : 303-11.

상세보기
Lodovici M, Guglielmi F, Meoni M and Dolara P. Effect of natural phenolic acids on DNA oxidation in vitro. Food Chem. Toxicol. 2001 ; 39 : 1205-10.

상세보기
Iontcheva I, Amar S, Zawawi KH, Kantarci A and Van Dyke TE. Role for moesin in lipopolysaccharidestimulated signal transduction. Infect. Immun. 2004 ; 72 : 2312-20.

상세보기
Li Y, Wu Q, Deng Y, Lv H, Qiu J, Chi G and Feng H. D(-)-salicin inhibits the LPS-induced inflammation in RAW264.7 cells and mouse models. Int. Immunopharmacol. 2015 ; 26 : 286-94.

상세보기
Bosca L, Zeini M, Traves PG and Hortelano S. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 2005 ; 208 : 249-58.

상세보기
Nathan C and Xie QW. Nitric oxide synthases: role, tolls, and control. Cell. 1994 ; 78 : 915-8.

상세보기
Wei W, Li XY, Zhang HQ and Wu SG. Anti inflammatory and immuno pharmacology. 1sted. Beijing : Renmin weisheng chubanshe. 2004 ; 10-7.
Kim JY and Chol JY. Anti-inflammatory effects of ginseng-derived active principles. The Korean Ginseng Research and Industry. 2007 ; 1 : 17-23.
Park WS. Effect of water extract from artemisiae argi folium on mouse macrophage stimulated by LPS. Kor. J. Herbology. 2009 ; 24 : 151-7.
Yoon SB, Lee YJ, Park SK, Kim HC, Bae H, Kim HM, Ko SG, Choi HY, Oh MS and Park W. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J. Ethnopharmacol. 2009 ; 125 : 286-90.

상세보기
Lee JY, Lee YJ and Park WS. Anti-inflammatory Effects of Fermented Houttuyniae Herba Water Extract on LPS-induced Mouse Macrophage. Kor. J. Herbology. 2010 ; 25 : 27-34

원문보기 상세보기
Sung MS, Kim YH, Choi YM, Ham HM, Jeong HS and Lee JS. Anti-inflammatory effect of Erigeron annuus L. flower extract through heme oxygenase-1 induction in RAW264.7 macrophages. J Korean Soc Food Sci Nutr. 2011 ; 40 : 1507-11.

원문보기 상세보기
Lee JW. Antiarthritic and anti-inflammatory effects of the mixture of Cinnamon Cortex, Persica Semen and Mirabilite. Kyung Hee University. 2013.
Pang L and Hoult JRS. Repression of inducible nitric oxide synthase and cyclooxygenase-2 by prostaglandin E2 and other cyclic AMP stimulants in J774 macrophages. Biochem P harmacol. 1997 ; 53 : 493-500.

상세보기
Hinz B, Kraus V, Pahl A and Brune K. Salicylate metabolites inhibit cyclooxygenase-2 dependent prostaglandin E2 synthesis in murine macrophages. Biochem Biophys Res Commun. 2000 ; 274 : 197-202.

상세보기
Nathan C and Xie QW. Regulation of biosynthesis of nitric oxide. J Biol Chem. 1994 ; 269 : 13725-8.

상세보기
Witthoht T, Eckmann L, Kim JM and Kagnoff MF. 1998. Enteroinvasive bacteria directly activate expression of iNOS and NO production in human colon epithelial cell. Am J Physiol.dlf. 1998 ; 275 : 564-71.
Ryu JH, Ahn H, Kim JY and Kim YK. Inhibitory activity of plant extracts on nitric oxide synthesis in LPS activated macrophages. Phytotherapy Research. 2003 ; 17 : 485-9.

상세보기
Kranzhofer R, Browatzki M, Schmidt J and Kubler W. Angiotensin II activates the proinflammatory transcription factor nuclear factor-κB in human monocytes. Biochem Biophys Res Commun. 1999 ; 257 : 826-8.

상세보기
Robinson MJ and Cobb MH. Mitogen-activated protein kinase pathways. Curr. Opin. Cell Biol. 1997 ; 9 : 180-6.

상세보기
Johnson GL and Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 2002 ; 298 : 1911-2.

상세보기
Sahu BD, Kumar JM and Sistla R. Baicalein, a bioflavonoid, prevents cisplatin-induced acute kidney injury by up-regulating antioxidant defense and down-regulating the MAPKs and NF-κB pathways. Plos One. 2015 ; 10 : 1-19.
Lee ES, Ju HK, Moon TC, Lee E, Jahng Y, Lee SH and Chang HW. Inhibition of nitric oxide and tumor necrosis factor-alpha(TNF-alpha) production by prepenone compound through blockade of nuclear factor(NF)-κB activation in cultured murine macrophages. Biol. Pharm. Bull. 2004 ; 27 : 617-20.

상세보기
Waetzig V, Czeloth K, Hidding U, Mielke K, Kanzow M, Brecht S, Goetz M, Lucius R, Herdegen T and Hanisch UK. c-Jun N-terminal kinases(JNKs) mediate pro-inflammatory actions of microglia. Glia. 2005 ; 50 : 235-46.

상세보기
Nam JB, Lee MH, Choi HY, Sohn NW and Kang H. Effect of forsythiae fructus ethanol extract on inflammatory cytokine production and cellular signaling pathways in mouse macrophages. Korean Journal of Herbology. 2012 ; 27 : 59-64.
Kaminska B. MAPK signalling pathways as molecular targets for anti-inflammatory therapyrom molecular mechanisms to therapeutic benefits. Biochimica et Biophysica Acta. 2005 ; 1754 : 253-62.

상세보기
Lowenstein CJ and Snyder SH. Nitricoxide, a novel biologic messenger. Cell. 1992 ; 70 : 705-7.

상세보기
Gomez PF, Pillinger MH, Attur M, Marjanovic N, Dave M, Park J, Bingham CO, Al Mussawir H and Abramson SB. Resolution of inflammation: prostaglandin E2 dissociates nuclear trafficking of individual NF-kappa B subunits(p65, p50) in stimulated rheumatoid synovial fibroblasts. Journal of Immunology. 2005 ; 175 : 6924-30.

상세보기
Kim JI, Jin CY, Seo MJ, Lim HS, Lee YC, Joo WH, Choi BT, Jeong YK and Choi YH. Genistein-induced growth inhibition was associated with inhibition of cyclooxygenase-2 and telomerase activity in human cancer cells. J Life Sci. 2008 ; 18 : 884-90.

원문보기 상세보기

저자의 다른 논문 :

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format