[논문]Tyrosinase 발현 조절을 통한 마름열매 추출물의 Melanin 생성 억제 효과

김영주

doi:10.15230/scsk.2023.49.4.307

[국내논문] Tyrosinase 발현 조절을 통한 마름열매 추출물의 Melanin 생성 억제 효과
The Inhibitory Effects of Water Chestnut Extracts on Melanogenesis through Regulation of Tyrosinase Expression 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.49 no.4, 2023년, pp.307 - 312

김영주 (오산대학교, 뷰티코스메틱계열)

초록
AI-Helper

본 연구에서는 국내에서 자생하고 있는 Trapa natans var. bispinosa 열매인 마름(water chestnut)의 anti-pigmentation 효능을 확인하였다. 마우스 유래 melanocytes인 B16F10세포에 마름 70% 에탄올 추출물을 처리하여 멜라닌의 생성량을 확인한 결과 200 ㎍/mL 추출물을 처리시 100 ng/mL α-MSH를 처리한 대조군 (100%) 대비 43.26%로 멜라닌생성억제를 확인하였다. 또한 멜라닌합성에 주요한 단백질인 tyrosinase의 활성 및 발현을 100 ng/mL α-MSH를 처리한 대조군(100%) 대비 tyrosinase의 활성은 23.65%, tyrosinase의 발현은 62.35%로 억제됨을 확인하였다. 본 연구결과를 통해 마름 70% 에탄올 추출물은 α-MSH에 의한 melanin 생성을 방지하여 미백 기능성 소재로써 가치가 있는 것으로 사료된다.

Abstract ▼ AI-Helper

Water chestnut is fruits of Trapa natans var. bispinosa grown wild in Korea. In this study, water chestnut was investigated for anti-pigmentation. Treatment with ethanolic extracts of water chestnut significantly reduced production of melanin in α-MSH simulated B16F10 cells. At 200 ㎍/mL ethanolic extracts of water chestnut, melanin contents were repressed by 43.26% compared to the control group. Additionally, ethanolic extracts of water chestnut reduced expression and activity of tyrosinase, key enzyme in melanogenesis, in α-MSH simulated B16F10 cells. Ethanolic extracts of water chestnut downregulated tyrosinase activity and expression to 23.65% and 62.35%, respectively. These results suggest that ethanolic extracts of water chestnut might be used as a promising whitening ingredients for inhibition of α-MSH-induced melanin synthesis and pigmentation.

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표/그림 (4)

그림 Figure 1. The cytotoxicity of water chestnut extracts. B16F10 cells were treated with 70% ethanol extracts of water chestnut (concentration is μg/mL) for 48 h, 37 ℃. The result is presented as the mean ± SD of the experiment (N = 3). *p < 0.05 (vs. control).
그림 Figure 2. The effect of water chestnut extracts on melanin contents. B16F10 cells were treated with 70% ethanol extracts of water chestnut (concentration is μg/mL) for 48 h, 37 ℃. (A) relative melanin contents, (B) pallet. The result is presented as the mean ± SD of the experiment (N = 3). ^#p < 0.05 (vs. control), *p < 0.05 (vs. α-MSH treated B16F10 cells).
그림 Figure 3. The effect of water chestnut extracts on tyrosinase activity. B16F10 cells were treated with 70% ethanol extracts of water chestnut (concentration is μg/mL) for 48 h, 37 ℃. The result is presented as the mean ± SD of the experiment (N = 3). ^#p < 0.05 (vs. control), *p < 0 .05 (vs. α -M SH t reated B16F10 cells).
그림 Figure 4. The effect of water chestnut extracts on tyrosinase mRNA and protein expression. B16F10 cells were treated with 70% ethanol extracts of water chestnut (concentration is μg/mL) for 48 h, 37 ℃. (A) mRNA expression of tyrosinase, (B) protein expression of tyrosinase. The result is presented as the mean ± SD of the experiment (N = 3). ^#p < 0.05 (vs. control), *p < 0.05 (vs. α-MSH treated B16F10 cells).

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