[논문]Cedrol 및 펩타이드 3 종 Complex 의 In Vitro 효능 및 적용 제형의 입술 주름 개선 및 Volumization 효과에 대한 연구

강성수; 전승현; 이진용; 안명진; 강내규

doi:10.15230/scsk.2023.49.4.365

Cedrol 및 펩타이드 3 종 Complex 의 In Vitro 효능 및 적용 제형의 입술 주름 개선 및 Volumization 효과에 대한 연구
In Vitro Efficacies of Complex of Cedrol and Three Peptides, and Wrinkle Improvements and Lips Volumization effects of Applied Formulations 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.49 no.4, 2023년, pp.365 - 373

강성수 (LG 생활건강 기술연구원 R&I 연구소) , 전승현 (LG 생활건강 기술연구원 R&I 연구소) , 이진용 (LG 생활건강 기술연구원 R&I 연구소) , 안명진 (LG 생활건강 기술연구원 R&I 연구소) , 강내규 (LG 생활건강 기술연구원 R&I 연구소)

초록
AI-Helper

입술은 인간의 매력을 표현할 수 있는 대표적인 안면부 부위로써, 이에 대한 미적 관심은 인류 역사에서 항상 존재해왔다. 그러나 입술은 노화가 진행됨에 따라, 주름이 형성되고, 얇아지고, 그 볼륨이 감소하는 경향을 가지며, 이를 대처하기 위해 필러나 지방 이식 등 시술이 시행되곤 한다. 본 연구에서는 립세린^® 에 적용된 주 효능 컴플랙스인 cedrol과 펩타이드 3 종(acetyl hexapeptide-8, acetyl tetrapeptide-9, desamidocollagen)의 조합이 광노화 방어 및 입술 주름을 개선할 수 있음을 in vitro상에서 검증하였다. 피부 섬유아세포의 콜라겐과 엘라스틴 발현량을 향상, 광노화 조건에서 MMP 발현을 경감하는 효과를 검증하였다. 또한 지방 줄기 세포 분화 실험에서 지방 줄기 세포 분화 촉진 및 지방 생성량을 증대시킴으로, 입술 내 지방 조직량을 증가시킬 수 있음을 in vitro상에서 그 가능성을 확인하였다. 2 주간 사용된 인체 적용 시험에서도, 입술의 주름, 결, 탄력, 볼륨이 개선되는 효과를 확인하였다. 본 연구는 cedrol 및 펩타이드 3 종 조합이 입술의 다양한 노화 징후를 해결할 수 있는 효과적인 립케어 소재로써 활용 가능함을 검증하였다.

Abstract ▼ AI-Helper

Since the lips are a representative facial part that can express human attraction, aesthetic interest in them has always existed in human history. However, as lips age, they tend to form wrinkles, become thinner, and lose their volume. To counteract this phenomenon, medical procedures such as fillers or fat transplantation have been suggested. In this study, we verified that the one of main effective material complex of L G H&H L IPCERIN®, combination of cedrol, a sesquiterpene found in cedarwood, and three peptides (acetyl hexapeptide-8, acetyl tetrapeptide-9, and desamidocollagen) could ameliorate the photo-aging and reduce the wrinkles through in vitro experiments. The possibility of improving collagen and elastin expression in skin fibroblasts and reducing MMP expression under photoaging conditions was verified. In addition, it was confirmed that the amount of fat tissue in the lips can be increased by promoting adipose stem cell differentiation and increasing the amount of fat produced in the in vitro adipose stem cell differentiation experiment. Two weeks of human application tests confirmed that a combination of cedrol and peptides can improve the wrinkles, texture, elasticity, and volume of the lips. This study verified that the combination of cedrol and three peptides can be used as effective cosmetic materials to decrease the various signs of aging in the lips.

주제어

표/그림 (6)

표 Table 1. Concentrations of peptide complex which were applied to in vitro experiments. Each concentration refers to ingredient's (in parenthesis) concentration. All units are ppm
표 Table 2. Ingredients for lip balm formulation
그림 Figure 1. In vitro efficacy of cerol and peptides complex to skin fibroblast. (A) Collagen type I alpha 1 chain (COL) and elastin (ELN) gene expressions of HS68 when cedrol & peptides complex was treated. (B) Collagen type I alpha 1 chain (COL), elastin (ELN), matrix metalloproteinases 1 (MMP) gene expressions of HS68 under photo-aging model. Ultraviolet (UVA 15 mJ/cm², UVB 20 mJ/cm²) was irradiated. After treatment of 24 hours, cells were harvested and analyzed. * p < 0.05; error bar was shown; ppm, parts per million.
그림 Figure 2. Effect of cedrol and peptides complex on adipogenesis in human adipose-derived stem cells. Cedrol and peptides complexes and positive control rosiglitazone (l μM) were treated for 10 days. After differentiation, adipocytes were stained with Oil red O. (A) Quantification of adipocytes stained with Oil red O. Absorbance at 515 nm were measured. (B) Fluorescence microscopy image to verify adipogenesis and lipid droplets. The data of cedrol 1 ppm and Conc. 2 was shown. (C) Adipocyte spheroids cultured and differentiated for 10 days. The data of cedrol 1 ppm and Conc. 2 was shown. 50 μm scale bar (white) was shown. * p < 0.05; error bar was shown; ppm, parts per million.
그림 Figure 3. In vivo human application test and clinical assessments. Twenty four women had tested lip balm including cedrol and peptides complex for 2 weeks. Lips were analyzed by DSLR camera, antera 3D and cutometer. (A) Wrinkle improvements of lips. Lengths, average widths, average depths, and maximum depths were analyzed. The percentage differences were shown as bar graph. DLSR camera images and antera 3D's wrinkle analyzing mode image were shown. (B)Texture improvements of lips. Roughness (Ra) was measured using antera 3D. (C) Elasticity improvements of lips. R0 and R8 were measured to evaluate external elasticity and elastic restoring force using cutometer. (D) Volumization effects of cedrol & peptides complex. Using antera 3D's elevation mode, the volume of lips were analyzed. Statistical analysis were performed as described in material and method section.
그림 Figure 4. Self-assessment for lip balm including cedrol and peptides complex. Twenty four women had tested lip balm including cedrol and peptides complex for 2 weeks. After 2 weeks, subjects were asked how they recognized and satisfied the benefits and efficacies.

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