[논문]청폐사간탕(淸肺瀉肝湯)의 피부 항노화 효과에 대한 연구

송영채; 김희택; 전보희; 김용민

doi:10.6114/jkood.2023.36.1.040

[국내논문] 청폐사간탕(淸肺瀉肝湯)의 피부 항노화 효과에 대한 연구
Studies on Skin Anti-aging Effect of Chungpyesagan-tang(CPSGT) 원문보기

韓方眼耳鼻咽喉皮膚科學會誌 = The journal of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, v.36 no.1, 2023년, pp.40 - 49

송영채 (세명대학교 한의과대학 한방안이비인후피부과) , 김희택 (세명대학교 한의과대학 한방안이비인후피부과) , 전보희 (세명대학교 화장품과학과) , 김용민 (세명대학교 화장품뷰티생명공학부)

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Objectives : This study was conducted to confirm the skin anti-aging effect of Chungpyesagan-tang(CPSGT) extract. Methods : We performed MMT assay to confirm the cytotoxicity of CPSGT. After inducing matrix metalloproteinase-1(MMP-1) with tumor necrosis factor-α(TNF-α), we investigated mRNA expression and protein secretion of MMP-1 by real-time RT-PCR and ELISA. In addition, we measured the protein expression of mitogen-activated protein kinases(MAPKs) and transcription factors by western blot. Results : CPSGT was not cytotoxic at 25-800㎍/㎖. The mRNA expression and protein secretion of MMP-1 decreased when treated with CPSGT. The protein expression of p-ERK, p-JNK, p-p38 was decreased by CPSGT. In addition, the protein expression of p-c-jun and p-NF-κB, which are transcription factors, were also decreased. Conclusion : This suggests that CPSGT can inhibit MMP-1 and thus be a potential anti-aging substance.

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표/그림 (7)

표 Table 1. Contents of CPSGT
표 Table 2. Gene Name and Assay ID Number in Real-time PCR Analysis
그림 Fig. 1. The Effects of CPSGT on Cell Viability in Hs68 Cells Control: untreated group; 25, 50, 100, 200, 400, 800: treated with various concentrations of CPSGT(㎍/㎖)
그림 Fig. 2. The Effects of CPSGT on MMP-1 mRNA Expression and Secretion in TNF-α induced Hs68 Cells (A) CPSGT inhibited TNF-α induced MMP-1 mRNA expression. (B) CPSGT inhibited TNF-α induced MMP-1 protein secretion. Forskolin was positive control. ^#p<0.05, significantly different from the control. *p<0.05, significantly different from the TNF-α-treated condition.
그림 Fig. 3. The Effects of CPSGT on MAPKs Protein Expression in Hs68 Cells (A) CPSGT inhibited the phosphorylation of MAPKs. (B) Data analyzed using image J. Forskolin was positive control. ^#p<0.05, significantly different from the control. *p<0.05, significantly different from the TNF-α-treated condition.
그림 Fig. 4. The Effects of CPSGT on p-c-jun Protein Expression in Hs68 Cells (A) CPSGT inhibited the phosphorylation of c-jun. (B) Data analyzed using image J. Forskolin was positive control. ^#p<0.05, significantly different from the control. *p<0.05, significantly different from the TNF-α-treated condition.
그림 Fig. 5. The Effects of CPSGT on p-NF-κB Protein Expression in Hs68 Cells (A) CPSGT inhibited the phosphorylation of NF-κB. (B) Data analyzed using image J. Forskolin was positive control. ^#p<0.05, significantly different from the control. *p<0.05, significantly different from the TNF-α-treated condition.

참고문헌 (30)

Baumann L. Skin ageing and its treatment. J？Pathol. 2007;211(2):241-51.
Lee CS, Bae IH, Han J, Choi G, Hwang KH,？Kim DH, et al. Compound K inhibits MMP-1？expression through suppression of？c-Src-dependent ERK activation in TNF-α-stimulated dermal fibroblast. Exp Dermatol.？2014;23(11):819-24.

상세보기
Yoon Y, Bae S, An S, Choe YB, Ahn KJ, An？IS. Effects of Ultraviolet Radiation on the？Skin and Skin Cell Signaling Pathways. Kor J？Aesthet Cosmetol. 2013;11(3):417-26.
Quan T, Fisher GJ. Role of age-associated？alterations of the dermal extracellular matrix？microenvironment in human skin aging: a？mini-review. Gerontology. 2015;61(5):427-34.

상세보기
Quan T, Qin Z, Xia W, Shao Y, Voorhees JJ.？Matrix-Degrading Metalloproteinases in？Photoaging. J Investig Dermatol Symp Proc.？2009;14(1):20-4.

상세보기
Jian J, Pelle E, Yang Q, Pernodet N, Maes D,？Huang X. Iron sensitizes keratinocytes and fi？broblasts to UVA-mediated matrix metallopr？oteinase-1 through TNF-α and ERK activatio？n. Exp Dermatol. 2011;20(3):249-54.

상세보기
Rittie L, Fisher GJ. UV-light-induced signal？cascades and skin aging. Ageing Res Rev.？2022;1(4):705-20.

상세보기
Pillai S, Oresajo C, Hayward J. Ultraviolet？radiation and skin aging: roles of reactive？oxygen species, inflammation and protease？activation, and strategies for prevention of？inflammation-induced matrix degradation -？a review. Int J Cosmet Sci. 2005;27(1):17-34.

상세보기
Lee JM. Dongeuisusebowon. Seoul:Henglim？Publishing Inc. 1986:7-138.
National Korean Medicine University？Textbook Editing Board. Sasang Medicine.？2nd ed. Seoul:Jipmoondang. 2011:280-300.
Lee JH, Lee EJ. Clinical Practice Guideline for？Taeeumin and Taeyangin Disease of Sasang？Constitutional Medicine: Diagnosis and？Algorithm. J Sasang Constitut Med.？2015;27(1):13-41.

원문보기 상세보기
Jeon SH, Yu JS, Lee EJ. Clinical Practice？Guideline for Taeeumin Disease of Sasang？Constitutional Medicine: Liver Heat-based？Interior Heat (Gansuyeol-liyeol) disease. J？Sasang Constitut Med. 2015;27(1):57-70.
Choi DJ, Jung WS, Moon SK, Cho KH, Kim？YS, Bae HS. The Clinical Efficacy of？Chungpyesagan-tang on Acute Stroke. J？Korean Oriental Med. 2002;23(4):9-14.
Park YJ, Kim SJ, Yang GE, Lee MJ, Lee JS,？Kang DH, et al. Anti-platelet Aggregation？Effect of Cheongpyesagan-tang in Vitro.？Korean J Orient Int Med. 2010;31(4):714-21.
Hong SG, Kang BJ, Kim YJ, Kang SM, Cho？DW. Protective effects of Chungpesagantang against ischemia/reperfusion induced？cell injury. Korean J Orient Int Med.？1999;5(1):111-7.
Oh YH, Jeong SH, Park IS, Shin GC, Lee WC.？The Effects of Chungpesagan-Tang Extracts？on Reversible Forebrain Ischemia in？Sprague-Dawley Rats. Korean J Orient Int？Med. 2001;22(2):161-74.
Kim JH, Park SS. The Effect of？Chungpyesagantang on Lipopolysaccharide？induced Arthritis in Mice. J Sasang Constitut？Med. 2002;14(3):114-31.
Kim EY, Sul YK, Choi JJ, Jeon SH, Kim H,？Kim JW. Effect of Cheongpesagan-tang on？the change of inhibitory effect against lipase？activity and weight loss, plasma and UCP1, 2？mRNA expression in db/db mouse. J Sasang？Constitut Med. 2007;19(1):171-85.
Bae JH, Lee JS, Chung SH, Kim SS, Shin HD.？Effect of Cheongpesagan-tang on the？changes of the weight, plasma and UCP？mRNAexpression in obese SD rats induced？high fat feed. J orient Rehabil Med.？2002;12(1):133-56.
Koo JS, Kim JH. Effects of Chungpyesaganta？ng on the diabetic rats induced by streptozo？tocin. J Korean Orient Pediatr. 1997;11(1):227-48.
Jeon BH, Kim TJ, Kim HT, Kim YM.？Anti-oxidative Effect of Chungpyesagan-tang？in LPS Induced RAW 264.7 Cells. J Korean？Med Ophthalmol Otolaryngol Dermatol.？2021;34(4):24-36.
Egbert M, Ruetze M, Sattler M, Wenck H,？Gallinat S, Lucius R, et al. The matricellular？protein periostin contributes to proper？collagen function and is downregulated？during skin aging. J Dermatol Sci.？2014;73(1):40-8.

상세보기
Lee J, Jung E, Lee J, Huh S, Hwang CH, Lee？HY, et al. Emodin inhibits TNF α-induced？MMP-1 expression through suppression of？activator protein-1(AP-1). Life Sci. 2006;79(26):2480-5.

상세보기
Vincenti MP, Brinckerhoff CE.？Transcriptional regulation of collagenase？(MMP-1, MMP-13) genes in arthritis:？integration of complex signaling pathways？for the recruitment of gene-specific？transcription factors. Arthritis Res. 2002;4(3):157-64.
Reunanen N, Li SP, Ahonen M, Foschi M, H？an J, Kahari VM. Activation of p38α MAPK E？nhances Collagenase-1(Matrix Metalloprotei？nase(MMP)-1) and Stromelysin-1(MMP-3) Ex？pression by mRNA Stabilization. J Biol Che？m. 2002;277(35):32360-8.

상세보기
Fisher GJ, Datta S, Wang ZQ, Li XY, Quan T,？Chung JH, et al. c-Jun-dependent inhibition？of cutaneous procollagen transcription？following ultraviolet irradiation is reversed？by all-trans retinoic acid. J Clin Invest.？2000;106(5):663-70.

상세보기
Fisher GJ, Talwar HS, Lin J, Lin P, McPhillips？F, Wang Z, et al. Retinoic Acid Inhibits？Induction of c-Jun Protein by Ultraviolet？Radiation that Occurs Subsequent to？Activation of Mitogen-activated Protein？Kinase Pathways in Human Skin In Vivo. J？Clin Invest. 1998;101(6):1432-40.
Noh EM, Lee G, Lim CH, Kwon KB, Kim JM,？Song HK, et al. Protective effects of Evodiae？Fructus extract against ultraviolet-induced？MMP-1 and MMP-3 expression in human？dermal fibroblasts. J Herb Med. 2022;35:100586.

상세보기
Bae S, Jung Y, Choi YM, Li S. Effects of？Er-Miao-San extracts on TNF-alpha-induced？MMP-1 expression in human dermal？fibroblasts. Biol Res. 2015;48:1-8.

상세보기
Jung YR, Kim DH, Kim SR, An HJ, Lee EK,？Tanaka T, et al. Anti-Wrinkle Effect of？Magnesium Lithospermate B from Salvia？miltiorrhiza BUNGE: Inhibition of MMPs via？NF-kB Signaling. PLoS ONE. 2014;9(8):e102689.

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[국내논문] 청폐사간탕(淸肺瀉肝湯)의 피부 항노화 효과에 대한 연구
Studies on Skin Anti-aging Effect of Chungpyesagan-tang(CPSGT) 원문보기

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[국내논문] 청폐사간탕(淸肺瀉肝湯)의 피부 항노화 효과에 대한 연구 Studies on Skin Anti-aging Effect of Chungpyesagan-tang(CPSGT) 원문보기

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[국내논문] 청폐사간탕(淸肺瀉肝湯)의 피부 항노화 효과에 대한 연구
Studies on Skin Anti-aging Effect of Chungpyesagan-tang(CPSGT) 원문보기

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