[논문]Effects on Goat Meat Extracts on α-Glucosidase Inhibitory Activity, Expression of Bcl-2-Associated X (BAX), p53, and p21 in Cell Line and Expression of Atrogin-1, Muscle Atrophy F-Box (MAFbx), Muscle RING-Finger Protein-1 (MuRF-1), and Myosin Heavy Chain-7 (MYH-7) in C2C12 Myoblsts

Joohyun Kang; Soyeon Kim; Yewon Lee; Jei Oh; Yohan Yoon

doi:10.5851/kosfa.2023.e6

Effects on Goat Meat Extracts on α-Glucosidase Inhibitory Activity, Expression of Bcl-2-Associated X (BAX), p53, and p21 in Cell Line and Expression of Atrogin-1, Muscle Atrophy F-Box (MAFbx), Muscle RING-Finger Protein-1 (MuRF-1), and Myosin Heavy Chain-7 (MYH-7) in C2C12 Myoblsts 원문보기

Food science of animal resources, v.43 no.2, 2023년, pp.359 - 373

Joohyun Kang (Department of Food and Nutrition, Sookmyung Women's University) , Soyeon Kim (Department of Food and Nutrition, Sookmyung Women's University) , Yewon Lee (Department of Food and Nutrition, Sookmyung Women's University) , Jei Oh (Department of Food and Nutrition, Sookmyung Women's University) , Yohan Yoon (Department of Food and Nutrition, Sookmyung Women's University)

Abstract ▼ AI-Helper

This study examined the α-glucosidase inhibitory, and apoptosis- and anti-muscular-related factors of goat meat extracts from forelegs, hind legs, loin, and ribs. The goat meat extracts were evaluated for their α-glucosidase inhibitory activity. The gene and protein expression levels of Bcl-2-associated X (bax), p53, and p21 were examined by reverse transcription polymerase chain reaction (RT-PCR) and immunoblotting in AGS and HT-29 cells. The expression levels of Atrogin-1 and MHC1b were examined by RT-PCR in C2C12 myoblasts, and the expression levels of Atrogin-1, muscle atrophy F-box (MAFbx), muscle RING-finger protein-1 (MuRF-1), and myosin heavy chain-7 were investigated by immunoblotting. α-Glucosidase inhibitory activity was higher in ethanol extract than in hydrous and hot water extracts. BAX and p53 expression levels were higher (p<0.05) in AGS cells treated with goat meat extract than those of cells treated with no goat meat extract. In HT-29 cells, the protein expression levels of BAX, p53, and p21 were higher (p<0.05) in the cells treated with goat meat extract than those of cells not treated with goat meat extract. In dexamethasone-treated C2C12 cells, goat meat extract treatment lower (p<0.05) the expression of Atrogin-1 and lower (p<0.05) the expression of MAFbx and MuRF-1. The results of the present study indicate that goat meat extracts have α-glucosidase inhibitory activity in vitro. In addition, apoptosis was induced in AGS cells and HT-29 cells treated with goat meat extract, and anti-muscular atrophy activity was also observed in C2C12 cells treated with goat meat extract.

주제어

표/그림 (11)

표 Table 1. Primer sequences for anti-cancer-related genes
표 Table 2. Primer sequences used in C2C12 cells for anti-muscle atrophy-related genes
그림 Fig. 1. α-Glucosidase inhibitory activity of goat meat at hydrous extract, hot water extract, and ethanol extract. ^a-e Values are significantly different (p<0.05).
그림 Fig. 2. Anti-cancer-related gene levels in HT-29 cells treated with goat hind legs at hydrous extract (HE), hot water extract (HWE), and ethanol extract (EE). (A) Bcl-2-associated X (bax), (B) p53, and (C) p21. ^a,b Values are significantly different (p<0.05).
그림 Fig. 3. Anti-cancer-related gene levels in AGS cells treated with goat meat extract. (A) Bcl-2-associated X (bax), (B) p53, and (C) p21. ^a,b Values are significantly different (p<0.05).
그림 Fig. 4. Anti-cancer-related protein levels in AGS cells treated with goat meat extract. (A) Bcl-2-associated X (BAX), (B) p53, and (C) p21. ^a,b Values are significantly different (p<0.05).
그림 Fig. 5. Anti-cancer-related gene levels in HT-29 cells treated with goat meat extract. (A) Bcl-2-associated X (bax), (B) p53, and (C) p21. ^a,b Values are significantly different (p<0.05).
그림 Fig. 6. Anti-cancer-related protein levels in HT-29 cells treated with goat meat extract. (A) Bcl-2-associated X (BAX), (B) p53, and (C) p21. ^a,b Values are significantly different (p<0.05).
그림 Fig. 7. Anti-muscular atrophy-related gene levels in C2C12 cells treated with goat hind legs at hydrous extract (HE), hot water extract (HWE), and ethanol extract (EE). (A) Atrogin-1 and (B) myosin heavy chain (MHC) 1b. ^a,b Values are significantly different (p<0.05).
그림 Fig. 8. Anti-muscular atrophy-related gene levels in C2C12 cells treated with goat meat extract. (A) Atrogin-1 and (B) myosin heavy chain (MHC) 1b. ^a-c Values are significantly different (p<0.05).
그림 Fig. 9. Anti-muscular atrophy-related protein levels in C2C12 cells treated with goat meat extract. (A) Atrogin-1, (B) muscle atrophy Fbox (MAFbx), (C) muscle RING-finger protein-1 (MuRF-1), and (D) myosin heavy chain-7 (MYH-7). ^a,b Values are significantly different (p<0.05).

AI 본문요약
AI-Helper

제안 방법

Therefore, this study evaluated the effects of extracts from the meat of goat forelegs, hind legs, loin, and ribs on α-glucosidase inhibitory activity and the expression levels of the apoptosis-related proteins [Bcl-2-associated X (BAX), p53, and p21] in AGS cells and HT-29 cells

성능/효과

Therefore, this study evaluated the effects of extracts from the meat of goat forelegs, hind legs, loin, and ribs on α-glucosidase inhibitory activity and the expression levels of the apoptosis-related proteins [Bcl-2-associated X (BAX), p53, and p21] in AGS cells and HT-29 cells. Further, the expression of muscle atrophy proteins [Atrogin-1, muscle atrophy F-box (MAFbx), muscle RING-finger protein-1 (MuRF-1), and myosin heavy chain-7 (MYH-7)] were evaluated in C2C12 myoblasts.
HT-29 cells treated with EE of goat forelegs showed a tendency to increase the expression levels of bax and p53 compared to those of control (cells treated with no EE; Fig. 5).
In EE-treated HT-29 cells, especially with the forelegs, hind legs, loins, and ribs extracts, the expression levels of BAX were higher (p<0.05) than those of the control (Fig
This study demonstrated that the in vitro α-glucosidase inhibitory activity of goat meat extracts, and expression levels of BAX, p53 and p21 were increased in goat meat extract-treated AGS and HT-29 cells; in contrast, the expression levels of MAFbx and MuRF-1 in C2C12 myoblasts were decreased
p53 expression levels were higher (p<0.05) in cells treated with EE of the forelegs and loin, and p21 expression levels in those treated with loin EE were higher (p<0.05) compared to those of control.

후속연구

These results were obtained only from in vitro experiments. Thus, the results need to be verified in the experiment of in vivo in a future study.

참고문헌 (42)

Anaeto M Adeyeye JA Chioma GO Olarinmoye AO Tayo GO 2010 Goat products: Meeting the challenges of human health and nutrition Agric Biol J N Am 1 1231 1236 10.5251/abjna.2010.1.6.1231.1236
Balagopal P Rooyackers OE Adey DB Ades PA Nair KS 1997 Effects of aging on in vivo synthesis of skeletal muscle myosin heavy-chain and sarcoplasmic protein in humans Am J Physiol 273 E790 E800 10.1152/ajpendo.1997.273.4.E790 9357810

상세보기
Bodine SC Baehr LM 2014 Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1 Am J Physiol Endocrinol Metab 307 E469 E484 10.1152/ajpendo.00204.2014 25096180

상세보기
Brown DM Parr T Brameld JM 2012 Myosin heavy chain mRNA isoforms are expressed in two distinct cohorts during C2C12 myogenesis J Muscle Res Cell Motil 32 383 390 10.1007/s10974-011-9267-4 22012579

상세보기
Ebert SM Al-Zougbi A Bodine SC Adams CM 2019 Skeletal muscle atrophy: Discovery of mechanisms and potential therapies Physiology 34 232 239 10.1152/physiol.00003.2019 31165685

상세보기
El-Deiry WS Harper JW O'Connor PM Velculescu VE Canman CE Jackman J Pietenpol JA Burrell M Hill DE Wang Y Wiman KG Mercer WE Kastan MB Kohn KW Elledge SJ Kinzler KW Vogelstein B 1994 WAF1/CIP1 is induced in p53 -mediated G 1 arrest and apoptosis Cancer Res 54 1169 1174 8118801

상세보기
El-Deiry WS Tokino T Velculescu VE Levy DB Parsons R Trent JM Lin D Mercer WE Kinzler KW Vogelstein B 1993 WAF1 , a potential mediator of p53 tumor suppression Cell 75 817 825 10.1016/0092-8674(93)90500-P 8242752

상세보기
Galanos P Pappas G Polyzos A Kotsinas A Svolaki I Giakoumakis NN Glytsou C Pateras IS Swain U Souliotis VL Georgakilas AG Geacintov N Scorrano L Lukas C Lukas J Livneh Z Lygerou Z Chowdhury D Sørensen CS Bartek J Gorgoulis VG 2018 Mutational signatures reveal the role of RAD52 in p53-independent p21-driven genomic instability Genome Biol 19 37 10.1186/s13059-018-1401-9 29548335

상세보기
Gan Z Rumsey J Hazen BC Lai L Leone TC Vega RB Xie H Conley KE Auwerx J Smith SR Olson EN Kralli A Kelly DP 2013 Nuclear receptor/microRNA circuitry links muscle fiber type to energy metabolism J Clin Invest 123 2564 2575 10.1172/JCI67652 23676496

상세보기
Gnanapradeepan K Basu S Barnoud T Budina-Kolomets A Kung CP Murphy ME 2018 The p53 tumor suppressor in the control of metabolism and ferroptosis Front Endocrinol 9 124 10.3389/fendo.2018.00124 29695998

상세보기
Gumucio JP Mendias CL 2013 Atrogin-1, MuRF-1, and sarcopenia Endocrine 43 12 21 10.1007/s12020-012-9751-7 22815045

상세보기
Haegens A Schols AM van Essen AL van Loon LJ Langen RC 2012 Leucine induces myofibrillar protein accretion in cultured skeletal muscle through mTOR dependent and -independent control of myosin heavy chain mRNA levels Mol Nutr Food Res 56 741 752 10.1002/mnfr.201100695 22648621

상세보기
Herningtyas EH Okimura Y Handayaningsih AE Yamamoto D Maki T Iida K Takahashi Y Kaji H Chihara K 2008 Branched-chain amino acids and arginine suppress MaFbx/atrogin-1 mRNA expression via mTOR pathway in C2C12 cell line Biochim Biophys Acta Gen Subj 1780 1115 1120 10.1016/j.bbagen.2008.06.004 18616983

상세보기
Hong H Lim JM Kothari D Kwon SH Kwon HC Han SG Kim SK 2021 Antioxidant properties and diet-related α -glucosidase and lipase inhibitory activities of yogurt supplemented with safflower ( Carthamus tinctorius L.) petal extract Food Sci Anim Resour 41 122 134 10.5851/kosfa.2020.e88 33506222

원문보기 상세보기
Hu W Kavanagh JJ 2003 Anticancer therapy targeting the apoptotic pathway Lancet Oncol 4 721 729 10.1016/S1470-2045(03)01277-4 14662428

상세보기
Hwang YH Bakhsh A Lee JG Joo ST 2019 Differences in muscle fiber characteristics and meat quality by muscle type and age of Korean native Black Goat Food Sci Anim Resour 39 988 999 10.5851/kosfa.2019.e92 31950114

원문보기 상세보기
Ivanović S Pavlović I Pasinov B 2016 The quality of goat meat and it's impact on human health Biotechnol Anim Husb 32 111 122 10.2298/BAH1602111I

상세보기
Jäättelä M 2002 Programmed cell death: Many ways for cells to die decently Ann Med 34 480 488 10.1080/078538902321012423 12523503

상세보기
Jeon SK Kim OH Park SM Lee JH Park SD 2020 Effects of glucoraphanin in dexamethasone-induced skeletal muscle atrophy in vitro model Herb Formula Sci 28 29 39
Jo K Jang WY Yun BS Kim JS Lee HS Chang YB Suh HJ 2021 Effect of deer antler extract on muscle differentiation and 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR)-induced muscle atrophy in C2C12 cells Food Sci Anim Resour 41 623 635 10.5851/kosfa.2021.e20 34291211

원문보기 상세보기
Kim BK Ha JJ Lee JK Oh DY Jung DJ Kim DH Hwang EG 2017 Effects of dietary addition of pegmatite on the meat quality characteristics of Hanwoo steers Korean J Food Nutr 30 1235 1244
Kim S Hong SP Lim SD 2021 Physiological characteristics and anti-diabetic effect of Pediococcus pentosaceus KI62 Food Sci Anim Resour 41 274 287 10.5851/kosfa.2020.e99 33987548

원문보기 상세보기
Lee J Park JR Jang A Yang SR 2019 The anti-proliferation and oxidative damage-related mechanism of L-carnitine in human colorectal cancer cells J Food Hyg Saf 34 303 308 10.13103/JFHS.2019.34.3.303

원문보기 상세보기
Malekian F Khachaturyan M Gebrelul S Henson JF 2014 Composition and fatty acid profile of goat meat sausages with added rice bran Int J Food Sci 2014 686298 10.1155/2014/686298 26904643

상세보기
Mazhangara IR Chivandi E Mupangwa JF Muchenje V 2019 The potential of goat meat in the red meat industry Sustainability 11 3671 10.3390/su11133671

상세보기
Monteiro A Costa JM Lima MJ 2017 Goat system productions: Advantages and disadvantages to the animal, environment and farmer Goat science Kukovics S (ed) IntechOpen London, UK 351 366 10.5772/intechopen.70002
Özbolat SN Ayna A 2021 Chrysin suppresses HT-29 cell death induced by diclofenac through apoptosis and oxidative damage Nutr Cancer 73 1419 1428 10.1080/01635581.2020.1801775 32757685

상세보기
Pellett PL Young VR 1990 Role of meat as a source of protein and essential amino acids in human protein nutrition Adv Meat Res 6 329 370
Perumalsamy H Sankarapandian K Veerappan K Natarajan S Kandaswamy N Thangavelu L Balusamy SR 2018 In silico and in vitro analysis of coumarin derivative induced anticancer effects by undergoing intrinsic pathway mediated apoptosis in human stomach cancer Phytomedicine 46 119 130 10.1016/j.phymed.2018.04.021 30097112

상세보기
Rajab P Fox J Riaz S Tomlinson D Ball D Greenhaff PL 2000 Skeletal muscle myosin heavy chain isoforms and energy metabolism after clenbuterol treatment in the rat Am J Physiol Regul Integr Comp Physiol 279 R1076 R1081 10.1152/ajpregu.2000.279.3.R1076 10956268

상세보기
Reed JC 2002 Apoptosis-based therapies Nat Rev Drug Discov 1 111 121 10.1038/nrd726 12120092

상세보기
Robertson RP Harmon J Tran POT Poitout V 2004 β-Cell glucose toxicity, lipotoxicity, and chronic oxidative stress in type 2 diabetes Diabetes 53 S119 S124 10.2337/diabetes.53.2007.S119 14749276

상세보기
Saltin B Henriksson J Nygaard E Andersen P Jansson E 1977 Fiber types and metabolic potentials of skeletal muscles in sedentary man and endurance runners Ann N Y Acad Sci 301 3 29 10.1111/j.1749-6632.1977.tb38182.x 73362

상세보기
Sans P Combris P 2015 World meat consumption patterns: An overview of the last fifty years (1961–2011) Meat Sci 109 106 111 10.1016/j.meatsci.2015.05.012 26117396

상세보기
Shija DS Mtenga LA Kimambo AE Laswai GH Mushi DE Mgheni DM Mwilawa AJ Shirima EJM Safari JG 2013 Chemical composition and meat quality attributes of indigenous sheep and goats from traditional production system in Tanzania Asian-Australas J Anim Sci 26 295 302 10.5713/ajas.2012.12432 25049790

원문보기 상세보기
Si M Lou J Zhou CX Shen JN Wu HH Yang B He QJ Wu HS 2010 Insulin releasing and alpha-glucosidase inhibitory activity of ethyl acetate fraction of Acorus calamus in vitro and in vivo J Ethnopharmacol 128 154 159 10.1016/j.jep.2009.12.044 20051258

상세보기
Sultan KR Henkel B Terlou M Haagsman HP 2006 Quantification of hormone-induced atrophy of large myotubes from C2C12 and L6 cells: Atrophy-inducible and atrophy-resistant C2C12 myotubes Am J Physiol Cell Physiol 290 C650 C659 10.1152/ajpcell.00163.2005 16176969

상세보기
Wan J Chen D Yu B Luo Y Mao X Zheng P Yu J Luo J He J 2017 Leucine protects against skeletal muscle atrophy in lipopolysaccharide-challenged rats J Med Food 20 93 101 10.1089/jmf.2016.3759 28009536

상세보기
Weglarz L Molin I Orchel A Parfiniewicz B Dzierżewicz Z 2006 Quantitative analysis of the level of p53 and p21 WAF1 mRNA in human colon cancer HT-29 cells treated with inositol hexaphosphate Acta Biochim Pol 53 349 356 10.18388/abp.2006_3348 16733561

상세보기
Yamabe N Kang KS Lee AY Lee D Choi JM Lee S Park JY Hwang GS Kim HY Cho EJ Lee S 2014 Identification of anti-cancer active components of Taraxacum coreanum on human gastric cancer AGS cells J Korean Soc Appl Biol Chem 57 187 190 10.1007/s13765-014-4031-2

원문보기 상세보기
Yoo IJ Kim KH Kim YE Park WM 1990 Effects of extraction time, temperature and amount of added water on beef extracts by hot water Korean J Food Sci Technol 22 858 864
Zierath JR Hawley JA 2004 Skeletal muscle fiber type: Influence on contractile and metabolic properties PLOS BIOL 2 e348 10.1371/journal.pbio.0020348 15486583

상세보기

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