[논문]미세먼지 유발 폐기능 손상 동물모델에서 RML의 호흡기 보호 효과

김수현; 김민주; 신미래; 노성수; 김승형; 박해진

doi:10.6116/kjh.2022.37.3.29.

미세먼지 유발 폐기능 손상 동물모델에서 RML의 호흡기 보호 효과
Respiratory Protective Effect of a RML on PM10D-induced Lung Injury Mouse Model 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.3, 2022년, pp.29 - 39

김수현 (대구한의대학교 한의과대학 본초약리학교실) , 김민주 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 노성수 (대구한의대학교 한의과대학 본초약리학교실) , 김승형 (대전대학교 동서생명과학연구원) , 박해진 (대구한의대학교 DHU바이오융복합시험센터)

Abstract ▼ AI-Helper

Objective : This study is aimed to evaluate the protective effects of Rehmanniae Radix, Mori Folium, and Liriopie Tuber mixture (RML) on lung injury of Particulate matter less than 10 um in diameter and diesel exhaust particles (PM10D) mice model. Methods : To investigate the anti-inflammatory activity of RML, PM10D was diluted in aluminum hydroxide (Alum) in 7-week-old male mice and induced by Intra-Nazal-Tracheal (INT) injection method. Animal experiments were divided into 5 groups. Nor (normal mice), CTL (PM10D-induced mice with the administration of distilled water), DEXA (PM10D-induced mice with the administration of 3 mg/kg Dexamethasone), RML 100 (PM10D-induced mice treated with RML 100 mg/kg weight), and RML 200 (PM10D-induced mice treated with RML 200 mg/kg body weight). After 11 days administration, mice were sacrificed and inflammation-related immune cells in broncho-alveolar lavage fluid (BALF) were analyzed. Inflammation-related biomarkers were also analyzed in blood and lungs. Lung tissue was observed through histological examination. Results : In the PM10D induced model, the PML showed decreases in CXCL-1 and IL-17A in BALF. Expression of inflammatory cytokines and cough-related mRNA genes was significantly decreased in serum and lung tissue. The mixture treatment of RML significantly improved the immune related cells in the serum. In addition, histological observations showed a tendency to decrease the severity of lung injury. Conclusions : Overall, these results confirmed the respiratory protective effect of the RML mixture in a model of lung injury induced by air pollution (PM10+DEP), suggesting that it is a potential treatment for respiratory damage.

주제어

표/그림 (10)

표 Table 1. Composition of RML
그림 Fig. 1. The animal experimental scheme.
표 Table 2. Sequence of Mouse Real-time PCR Oligonucleotide
그림 Fig. 2. Effect of RML on total cell in BALF, Lung. (A) Total bal cells; (B) Total lung cells. Nor, normal mice; CTL, PM10D-induced mice with the administration of distilled water; DEXA, PM10D-induced mice with the administration of 3 mg/kg Dexamethasone; RML 100, PM10Dinduced mice with the administration of 100 mg/kg RML; RML 200, PM10D-induced mice with the administration of 200 mg/kg RML. All values are mean±SEM (n=8). Significance: ^##P < 0.01 vs. normal mice, *P < 0.05, **P < 0.01 vs. PM10D-induced mice.
그림 Fig. 3. Effect of RML on CXCL-1 and IL-17A levels expression in BALF. (A) C-X-C Motif Chemokine Ligand 1 (CXCL-1); (B) Interleukin-17 (IL-17). Nor, normal mice; CTL, PM10D-induced mice with the administration of distilled water; DEXA, PM10D-induced mice with the administration of 3 mg/kg Dexamethasone; RML 100, PM10D-induced mice with the administration of 100 mg/kg RML; RML 200, PM10Dinduced mice with the administration of 200 mg/kg RML. All values are mean±SEM (n=8). Significance: ^###P < 0.001 vs. normal mice, *P < 0.05, **P < 0.01, ***P < 0.001 vs. PM10D-induced mice.
그림 Fig. 4. Effect of RML on SDMA in serum, and cough related Real-time PCR mRNA in Lung. (A) Symmetric dimethylarginine, (SDMA); (B) Mucin 5AC, (MUC5AC); (C) Transient receptor potential vanilloid 1, (TRPV1); (D) Transient receptor potential cation channel, subfamily A, member 1, (TRPA1). Nor, normal mice; CTL, PM10D-induced mice with the administration of distilled water; DEXA, PM10D-induced mice with the administration of 3 mg/kg Dexamethasone; RML 100, PM10D-induced mice with the administration of 100 mg/kg RML; RML 200, PM10D-induced mice with the administration of 200 mg/kg RML. All values are mean±SEM (n=8). Significance: ^##P < 0.01, ^###P < 0.001 vs. normal mice, *P < 0.05, **P < 0.01 vs. PM10D-induced mice.
그림 Fig. 5. Effect of RML on inflammatory mediator-related Real-time PCR mRNA in Lung. (A) Cyclooxygenase-2, (COX-2); (B) Nitric oxide synthase 2, (NOS-II); (C) Tumor necrosis factor-α, (TNF-α). Nor, normal mice; CTL, PM10D-induced mice with the administration of distilled water; DEXA, PM10D-induced mice with the administration of 3 mg/kg Dexamethasone; RML 100, PM10D-induced mice with the administration of 100 mg/kg RML; RML 200, PM10D-induced mice with the administration of 200 mg/kg RML. All values are mean±SEM (n=8). Significance: ^##P < 0.01, ^###P < 0.001 vs. normal mice, *P < 0.05, **P < 0.01 vs. PM10D-induced mice.
그림 Fig. 6. Effect of RML on immune related cell in Serum. (A) Lymphocyte and Neutrophils; (B) Monocytes and Eosinophils. Nor, normal mice; CTL, PM10D-induced mice with the administration of distilled water; DEXA, PM10D-induced mice with the administration of 3 mg/kg Dexamethasone; RML 100, PM10D-induced mice with the administration of 100 mg/kg RML; RML 200, PM10D-induced mice with the administration of 200 mg/kg RML. All values are mean±SEM (n=8). Significance: ^#P < 0.05, vs. normal mice, *P < 0.05, **P < 0.01, ***P < 0.001 vs. PM10D-induced mice.
그림 Fig. 7. Effect of RML on signal transduction TNF-α protein expression in lung. Nor, normal mice; CTL, PM10D-induced mice with the administration of distilled water; DEXA, PM10D-induced mice with the administration of 3 mg/kg Dexamethasone; RML 100, PM10Dinduced mice with the administration of 100 mg/kg RML; RML 200, PM10D-induced mice with the administration of 200 mg/kg RML.
그림 Fig. 8. Effect of GE on the histopathological change in lung tissue. lung tissue was stained with H&E (Original magnification ×200). Nor, normal mice; CTL, PM10D-induced mice with the administration of distilled water; DEXA, PM10D-induced mice with the administration of 3 mg/kg Dexamethasone; RML 100, PM10D-induced mice with the administration of 100 mg/kg RML; RML 200, PM10D-induced mice with the administration of 200 mg/kg RML.

참고문헌 (43)

Organization for Economic Cooperation and Development (OECD). The economic consequences of outdoor air pollution. 2016 [cited 2019 Nov 26]. Available from:https://www.oecd.org/environment/indicators-modelling-outlooks/Policy-Highlights-Economic-consequences-of-outdoor-air-pollution-web.pdf
Jo HJ, Park SW, Lee HI, Lee SW. Health effects caused by partic-ulate matter and guidelines for health care. Public Health Weekly Report. 2018 ; 11 : 458-62.
Song HW, Ji KY, Kim BK, Yang WK, Han CK, Shin HJ, Park YC, Hwang JS, Kang HS, Kim SH. Respiratory Protective Effect of Salvia plebeia R. Br. Extracts against Ambient Particulate Matter-induced Airway Inflammation. Korean J. Medicinal Crop Sci. 2017 ; 25(5) : 269-81.

원문보기 상세보기
WHO. WHO Expert Consultation: Available evidence for the fu-ture update of the WHO global air quality guidelines (AQGs). 2016 [cited 2019 Nov 26].Available from:http://www.euro.-who.int/en/health-topics/environment-and-health/air-quality/publications/2016/who-expert-consultation-available-evidence-for-the-future-update-of-the-who-global-air-quality-guidelines- aqgs-2016.
Brandt EB, Kovacic MB, Lee GB, Gibson AM, Acciani TH, Le Cras TD, Ryan PH, Budelsky AL, Hershey GKK. Diesel exhaust particle induction of IL-17A contributes to severe asthma. Journal of Allergy and Clinical Immunology. 2013 ; 132 : 1194-204.

상세보기
Doeing DC, Solway J. Airway smooth muscle in the pathophysiology and treatment of asthma. Journal of Applied Physiology. 2013 ; 114 : 834-43.

상세보기
Fuks K, Moebus S, Hertel S, Viehmann A, Nonnemacher M, Dragano N, Mohlenkamp S, Jakobs H, Kessler C, Erbel R, Hoffmann B. Long-term urban particulate air pollution, traffic, noise and arterial blood pressure. Environmental Health Perspectives. 2011 ; 119 : 1706-11.

상세보기
Peters S, Reid A, Fritschi L, de Klerk N, Musk AW. Long-term effects of aluminium dust inhalation. Occupational and Environmental Medicine. 2013 ; 70 : 864-8.

상세보기
Hoffmann B, Weinmayr G, Hennig F, Fuks K, Moebus S, Weimar C, Dragano N, Hermann DM, Kalsch H, Mahabadi AA, Erbel R, Jockel KH. Air quality, stroke, and coronary events: Results of the heinz nixdorf recall study from the ruhr region. Deutsches Arzteblatt International. 2015 ; 112 : 195-201.

상세보기
Ghio AJ, Smith CB, Madden MC. Diesel exhaust particles and airway inflammation. Current Opinion in Pulmonary Medicine. 2012 ; 18 : 144-50.

상세보기
Barnes PJ. Cytokines as mediators of chronic asthma. American Journal of Respiratory and Critical Care Medicine. 1994 ; 150 : S42-9.

상세보기
Bohadana AB, Massin N, Wild P, Toamain JP, Engel S, Goutet P. Symptoms, airway responsiveness, and exposure to dust in beech and oak wood workers. Occupational and Environmental Medicine. 2000 ; 57 : 268-73.

상세보기
Park YB, Rhee CK, Yoon HK, Oh YM, Lim SY, Lee JH, Yoo KH, Ahn JH. COPD clinical practice guideline of the Korean Academy of Tuberculosis and Respiratory Disease: a summary. Tuberc Respir Dis (Seoul). 2018 ; 81(4) : 261-73.

원문보기 상세보기
Kim TH, Yang WK, Lee SW, Kim SH, Lyu YR, Park YC. Inhibitory Effects of GGX on Lung Injury of Chronic Obstructive Lung Disease (COPD) Mice Model. J Korean Med. 2021 ; 42(3) : 56-71.

원문보기 상세보기
Chae BS, Shin TY. Effect of Fresh Rehmanniae Radix Methanol Extracts on the Production of Cytokines. Yakhak Hoeji. 2006 ; 50(1) : 184-90.
Herbology editorial committee of Korean medicine schools. Herbology [Boncho-hak]. Seoul : Young-Lim Press. 2016 : 232, 633.
Kim JS. Effect of Rhemanniae Radix on the Hyperglycemic Mice Induced with Streptozotocin. The Korean Society of Food Science and Nutrition. 2004 ; 33(7) : 1133-8.
Bae HK, Seo BI. Inhibitory Activities of Rehmanniae Radix 30% Ethanol Extract on Acute Gastritis and Peptic Ulcers. Kor. J. Herbol. 2019 ; 34(2) : 1-14.

원문보기 상세보기
Kwon YD, Song YS. Effects of Rhizoma rehmanniae adminstration on the Innate and Adap tive Responses in the Mouse. j. of Oriental Rehabilitation Medicine. 1998 ; 8(1) : 233-54.
Ju MJ, Kwon JH, Kim HK. Physiological activities of mulberry leaf and fruit extracts with different extraction conditions. Korean J Food Preserv. 2009 ; 16 : 442-8.
Byun SH, Park SM, Kim SC, Cho IJ. Anti-fibrotic Effect of Mori Folium Extract in Hepatic Stellate Cells. Kor J Herbology. 2013 ; 28(4) : 49-55.

원문보기 상세보기
Hunyadi A, Veres K, Danko B, Kele Z, Weber E, Hetenyi A, Zupko I, Hsieh T. In vitro anti-diabetic activity and chemical characterization of an apolar fraction of Morus alba leaf water extract. Phytother Res. 2013 ; 27(6) : 847-51.

상세보기
Naowaboot J, Pannangpetch P, Kukonqviriyapan V, Kongyingyoes B, Kukonqviriyapan U. Antihyperglycemic, antioxidant and antiglycation activities of mulberry leaf extract in streptozotocininduced chronic diabetic rats. Plant Foods Hum Nutr. 2009 ; 64 : 116-21.

상세보기
Freidwald WT, Levy RI, Fredrickson DS. Estimation of concentration of the low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972 ; 18(6) : 499-02.

상세보기
Editorial Committee of Oriental Medicine and Herbal Medicine. Herbal medicine. Seoul : Younglimsa. 2005 : 647-48.
National College of Korean Medicine Textbook Compilation Committee. Herbology. Seoul : Youglimsa. 2008 : 644-55.
Kim H. Effect of Maekmoondong-Tang on the immunomodulatory action. Korean Journal of Oriental Physiology & Pathology. 2003 ; 17 : 946-51.
Kim HK, Lee JY, Han HS, Kim YJ, Kim HJ, Kim YS, Kim HM, Ko SG, An HJ, Lee YJ, Park W. Immunomodulatory Effects of Liriope Platyphylla Water Extract on Lipopolysaccharide-Activated Mouse Macrophage. Nutrients. 2012 ; 4(12) : 1887-97.

상세보기
Ahn JH. Effect of Liriopis tuber on Liver function activity. Department of Pharmacy, Catholic University of Daegu. 2000.
Choi SB, Wha JD, Park S. The insulin sensitizing effect of homoisoflavone-enriched fraction in Liriope platyphylla Wang et Tang via PI3-kinase pathway. Life Sci. 2004 ; 75(22) : 2653-64.

상세보기
Huang TJ, Tsai YC, Chiang SY, Wang GJ, Kuo YC, Chang YC, Wu YY, Wu YC. Anti-viral effect of a compound isolated from Liriope platyphylla against hepatitis B virus in vitro. Virus Res. 2014 ; 192 : 16-24.

상세보기
Yoshitaka I. Research on the quality distribution of JIS type-II fly ash in Japan. World of Coal Ash. www.flyash.info/2007/67ishikawa.pdf (cited by 2007 May 7).
Lim HB, Kim SH. The effect of crude saponins of Korean red ginseng against airway inflammation and airway hyperresponsiveness induced by diesel exhaust particles in mice. Korean Journal of Medicinal Crop Science. 2009 ; 17 : 90-6.
Fonio, A. Uber ein neues Verfahren der Blutplattchenzahlung. Dtsch. Z. Chir. 1912 ; 117, 176.
Grace MS, Baxter M, Dubuis E, Birrell MA, Belvisi MG. Transient receptor potential (TRP) channels in the airway: role in airway disease. Br J Pharmacol. 2014 ; 171 : 2593-607.

상세보기
Wang G, Xu Z, Wang R, Al-Hijji M, Salit J, Strulovici-Barel Y, Tilley AE, Mezey JG, Crystal RG. Genes associated with MUC5AC expression in small airway epithelium of human smokers and nonsmokers. BMC Med Genomics. 2012 ; 5 : 21.

상세보기
van Manen MJG, Birring SS, Vancheri C, Cottin V, Renzoni EA, Russell AM, Wijsenbeek MS. Cough in idiopathic pulmonary fibrosis. European Respiratory Review. 2016 ; doi:10.1183/16000617.0090-2015.

상세보기
Park MK, Seong MG, Lee MK. Effects of TRPV1 in formalin-induced nociceptive behavior in theorofacial area of rats. Journal of the Korea Academia-Industrial cooperation Society. 2014 ; 15(1) : 316-22.

원문보기 상세보기
Son HJ, Kim YS, Misaka T, Noh BS, Rhyu MR. Activation of the Chemosensory Ion Channels TRPA1 and TRPV1 by Hydroalcohol Extract of Kalopanax pictus Leaves. Biomol Ther. 2012 ; 20(6) : 550-5.

원문보기 상세보기
Lukacs NW, Hogaboam CM, Kunkel SL. Chemokines and their receptors in chronic pulmonary disease. Curr Drug Targets Inflamm Allergy. 2005 ; 4(3) : 313-7.
Traves SL, Donnelly LE. Th17 cells in airway diseases. Curr Mol Med. 2008 ; 8(5) : 416-26.

상세보기
Ge Y, Huang M, Yao YM. Biology of Interleukin-17 and Its Pathophysiological Significance in Sepsis. Front. Immunol. 2020 ; doi.org/10.3389/fimmu.2020.01558.

상세보기
McCreanor J, Cullinan P, Nieuwenhuijsen MJ, Stewart-Evans J, Malliarou E, Jarup L, Harrington R, Svartengren M, Han IK, Ohman-Strickland P, Chung KF, Zhang J. Respiratory effects of exposure to diesel traffic in persons with asthma. N Engl J Med 2007 ; 357(23) : 2348-58.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증