[논문]국내 자생 해조류 추출물의 항산화능 비교분석 연구

김경; 이경하; 양혜원; 우채현; 정우혁; 박은영; 오윤신

doi:10.7318/kjfc/2023.38.2.112

국내 자생 해조류 추출물의 항산화능 비교분석 연구
Comparative Analysis of Antioxidant Activity of Korean Seaweeds Extracts 원문보기

韓國食生活文化學會誌 = Journal of the Korean Society of Food Culture, v.38 no.2, 2023년, pp.112 - 118

김경 (을지대학교 식품영양학과) , 이경하 (을지대학교 식품영양학과) , 양혜원 (을지대학교 식품영양학과) , 우채현 (을지대학교 식품영양학과) , 정우혁 (전남바이오산업진흥원 해양바이오연구센터) , 박은영 (목포대학교 약학대학) , 오윤신 (을지대학교 식품영양학과)

Abstract ▼ AI-Helper

Seaweed-derived foods have long been popular in Korea because of their high content of nutrients that are beneficial to the human body. Recently, Korean seaweeds have been used as raw materials to produce new natural products with health benefits. Herein, we compared the antioxidant activity of 16 Korean seaweed extracts to explore their potential utility as health foods. The total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of seaweed extracts were determined. We also investigated their ability to protect human diploid fibroblast (HDF) cells against hydrogen peroxide. The results showed that seaweed extracts at a concentration of 100 ㎍/mL did not cause any cell toxicity. Sargassum thunbergii (Jichung-i) had the highest TPC and radical scavenging effects, followed by Porphyra tenera (Gim), Silvetia siliquosa (Tteumbugi), and Sargassum fusiforme (Tot). Hydrogen peroxide increased the production of intracellular reactive oxygen species, while P. tenera (Gim), Saccharina japonica (Dasima), and S. thunbergii (Jichung-i) extracts significantly decreased it. The effect was highest in the S. thunbergii (Jichung-i)-treated HDF cells. These findings indicate that S. thunbergii (Jichung-i) shows promise as a potential antioxidant raw material.

주제어

표/그림 (5)

표 The list of Korean seaweeds used in this study
그림 Correlation between total phenolic content (TPC) and DPPH radical scavenging activity of 16 Korean seaweed extracts.
표 Total polyphenol content and DPPH radical scavenging effects of Korean seaweed extracts
그림 The Cytotoxicity to human diploid fibroblasts (HDF) at 100 mg/mL concentration of Korean seaweed extract. Cell viability was measured by MTT assay. The data are expressed as the mean±SD (n=3). CON, untreated cell; treated cell with Korean seaweed extracts (1~16); The name of Korean seaweed extracts was indicated as follows: 1, Neolpae; 2, Miyeog; 3, Jichung-i; 4, Tot; 5, Soemiyeog; 6, Tteumbugi; 7, Gwaengsaengi-mojaban; 8, Miyeoggwi; 9, Dasima; 10, Semogasali; 11, Gim; 12, Chamdobag; 13, Gasipalae; 14, Maesaeng-i; 15, Chungaac; 16, Palae.
그림 Effect of Korean seaweed extracts on H₂O₂-induced intracellular reactive oxygen species (ROS) generation in human diploid fibroblasts. Intracellular ROS generation was measured by the fluorescence microplate reader. Cells were pretreated with Korean seaweed extract for 24 hr and then exposed with 1.0 mM H₂O₂ for 1.5 hr. All values are presented as mean±SD. Bars with different letters indicate statistically significant differences among groups at p<0.05 by one-way ANOVA. CON, untreated cell; 1mM H₂O₂, treated cell with 1mM H₂O₂; co-treated cell with 1mM H₂O₂ + Korean seaweed extracts (No. 1~16); The name of Korean seaweed extracts was indicated as follows: 1, Neolpae; 2, Miyeog; 3, Jichung-i; 4, Tot; 5, Soemiyeog; 6, Tteumbugi; 7, Gwaengsaengi-mojaban; 8, Miyeoggwi; 9, Dasima; 10, Semogasali; 11, Gim; 12, Chamdobag; 13, Gasipalae; 14, Maesaeng-i; 15, Chungaac; 16, Palae.

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표제어: PCR

동의어: Packet Collision Rate

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