[논문]야생 산삼 지상부 및 지하부의 화학성분 분석과 피부 항노화 효능 평가

노석선; 이광진; 김병현; 황보경; 김효진; 장윤희; 유재군; 주영승

doi:10.6116/kjh.2023.38.5.85

[국내논문] 야생 산삼 지상부 및 지하부의 화학성분 분석과 피부 항노화 효능 평가
Analysis of Chemical Components for Aerial and Underground Parts of Wild Ginseng and Evaluation of Skin Anti-aging Efficacy 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.38 no.5, 2023년, pp.85 - 95

노석선 (대전대학교 한의과대학 한의학과) , 이광진 (LG생활건강 기술연구원) , 김병현 (LG생활건강 기술연구원) , 황보경 (LG생활건강 기술연구원) , 김효진 (LG생활건강 기술연구원) , 장윤희 (LG생활건강 기술연구원) , 유재군 (한국 산삼 중앙회) , 주영승 (우석대학교 한의과대학 한의학과)

Abstract ▼ AI-Helper

Objectives : This study was intended to reveal the chemical profiles of aerial(leaf, stem) and underground(rhizome, radix) parts of wild ginseng, and to investigate their anti-aging effects on human skin cells. Methods : Wild ginseng, estimated for over 20 years, was divided into the aerial and underground parts. Total phenolic contents of each extracts were measured using a Folin-ciocalteu method. The contents of 18 amino acids, 8 minerals and 27 ginsenosides were determined by GC-FID, ICP-MS and LC-MS, respectively. The anti-aging effects, including the radical scavenging activity, the activation of mitochondrial function on human fibroblasts, and the proliferation activity on human keratinocyte progenitor cells, for the whole plant and underground part of wild ginseng were evaluated. Results : The total phenolic acids, amino acids, and minerals in the aerial part were more than twice as high as in the underground part. Compared to the cultivated ginseng root, there were various types of ginsenosides in both parts of wild ginseng, and the total amount was more than twice as high. In particular, the aerial part significantly contained ginsenoside F1, F2, C-Mc1, and C-O, and the distinctive patterns that distinguish each parts of wild ginseng from the cultivated ginseng root were derived. The whole plant and underground part of wild ginseng exhibited significant antioxidant effect(14.3-45.6%), activation of mitochondrial membrane potential(105.5-120.1%), and cell proliferation(112.1-125.4%). Conclusions : The entire plant and underground part of wild ginseng are high value-added plants and have beneficial effects on skin anti-aging properties through its abundant metabolites.

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표/그림 (9)

그림 Fig. 1. Korean wild ginseng
표 Table 1. Contents of the total phenolic acids, amino acids, and minerals in the aerial and underground parts of wild ginseng.
표 Table 2. Contents of the 18 amino acids in the aerial and underground parts of wild ginseng.
표 Table 3. Contents of the 8 minerals in the aerial and underground parts of wild ginseng.
표 Table 4. Contents of the 27 ginsenosides in the aerial and underground parts of wild ginseng.
그림 Fig. 2. Radar charts of ginsenosides in three different ginseng samples; (A) underground part of P. ginseng cultivated for 6 years, (B) underground part of wild P. ginseng (an estimated 20-year-old wild ginseng), (C) aerial part of wild P. ginseng . The contents (mg/kg of dry weight) are plotted as log scales; values with a content of 1 mg/kg or less were considered zero in the scales.
그림 Fig. 3. Effect of the whole plant and underground part of wild ginseng on mitochondrial membrane potential of fibroblast cells. The MITO-ID^® Membrane potential detection kit was used for the assay, and 2 μM of carbonyl cyanide chlorophenylhydrazone CCCP) as a mitochondrial uncoupler was the negative control. Each bar in the graph represents mean ± S.D. and the p-values of all groups were below 0.05.
그림 Fig. 4. Cell proliferation activity of the whole plant and underground part of wild ginseng. Keratinocyte stem cell proliferation in basal media containing the extracts of wild ginseng was measured using CCK-8. KSCM (complete media for keratinocyte stem cell) was used as a positive control. Each bar in the graph represents mean ± S.D. and the p -values of all groups were below 0.05.
표 Table 5. DPPH radical scavenging activities of the whole plant and underground part of wild ginseng.

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