[논문]MPTP로 유도된 파킨슨병 생쥐 모델에 대한 시호계지탕, 시호소간탕, 시호청간탕의 신경세포 보호 효과 비교 연구

서지은; 이한울; 배창환; 윤동학; 김희영; 김승태

doi:10.14406/acu.2023.007

MPTP로 유도된 파킨슨병 생쥐 모델에 대한 시호계지탕, 시호소간탕, 시호청간탕의 신경세포 보호 효과 비교 연구
Comparative Study of the Neuroprotective Effect of Sihogyeji-tang, Sihosogan-tang, and Sihocheonggan-tang on an MPTP-Induced Parkinson's Disease Mouse Model 원문보기

Korean journal of acupuncture, v.40 no.3, 2023년, pp.90 - 98

서지은 (부산대학교 한의학전문대학원 한의과학과) , 이한울 (부산대학교 건강노화한의과학연구센터) , 배창환 (부산대학교 한의학전문대학원 한의과학과) , 윤동학 (경희윤동학한의원) , 김희영 (부산대학교 건강노화한의과학연구센터) , 김승태 (부산대학교 한의학전문대학원 한의과학과)

Abstract ▼ AI-Helper

Objectives : Parkinson's disease (PD) is the second most common neurodegenerative disorder worldwide and is characterized by the loss of the dopaminergic neurons in the substantia nigra (SN). In a previous in vitro study, we demonstrated that Sihogyeji-tang (SG), Sihosogan-tang (SS), and Sihocheonggan-tang (SC) have the potential to be candidate medicines for PD. This study aimed to compare the neuroprotective effect of SG, SS, and SC using 1-methyl-4-phenyl-1,2,3,6-tetrahydrophridine (MPTP)-induced PD mouse model. Methods : Eight-week-old male C57BL/6 mice were intraperitoneally administered with 30 mg/kg of MPTP for 5 days and orally administered SG, SS and SC for 12 days from the first MPTP injection. Motor function was assessed using the pole test and the rotarod test. Dopaminergic neuronal survival in the SN and striatum was evaluated through tyrosine-hydroxylase immunohistochemistry. Results : MPTP administration resulted in behavioral impairment and dopaminergic neuronal death in the SN and striatum. In the pole test, treatment with SG, SS, and SC alleviated the MPTP-induced motor dysfunction on day 5 and 12. In the rotarod test, SS and SG alleviated the MPTP-induced motor dysfunction on day 5, while only SS showed improvement on day 12. SS and SG significantly protected dopaminergic neurons in the SN from MPTP toxicity, and all three compounds (SG, SS, and SC) showed significant protection in the striatum. Notably, SS demonstrated superior efficacy in suppressing MPTP-induced motor dysfunction and dopaminergic neuronal death compared to SG and SC. Conclusions : These findings suggest that SS is the most effective formula among SG, SS, and SC for PD, indicating its potential role in the treatment of PD.

주제어

표/그림 (5)

그림 Fig. 1. Schedule of experiment. From day 1, the mice were intraperitoneally (i.p.) injected with 30 mg/kg of 1-methyl-4-phenyl-1,2,3,6-tetrahydrophridine (MPTP) per day for 5 days, and were orally (p.o.) administered vehicle or Korean medicine extract for 12 days. Behavior test was performed on days 0 (1 day prior to the first administration of MPTP) 5, and 12.
그림 Fig. 3. Latency to fall in the rotarod test. The time that the mice remained on the cylinder was measured on day 0, 5, and 12. NOR, saline-administrated group; MPTP, MPTP-administrated group; MPTP+SG, MPTP-administrated group with Sihogyegi-tang; MPTP+SS, MPTP-administrated group with Sihosogan-tang; MPTP+SC, MPTP-administrated group with Sihocheonggan-tang. Data presented as means±standard deviation. Means with different letters are significantly different (p<0.05) by Duncan's multiple-range test.
그림 Fig. 2. Total time taken in the pole test. NOR, saline-administrated group; MPTP, MPTP-administrated group; MPTP+SG, MPTP-administrated group with Sihogyegi-tang; MPTP+SS, MPTP-administrated group with Sihosogan-tang; MPTP+SC, MPTP-administrated group with Sihocheonggan-tang. Data presented as means±standard deviation. Means with different letters are significantly different (p<0.05) by Duncan's multiple-range test.
그림 Fig. 4. Comparision of the protective effect of Sihogyegi-tang, Sihosogan-tang, and Sihocheonggan-tang on dopaminergic neurons in the substantia nigra. Number of TH-positive cells in the substantia nigra was manually counted. TH, tyrosine hydroxylase; NOR, saline-administrated group; MPTP, MPTP-administrated group; MPTP+SG, MPTPadministrated group with Sihogyegi-tang; MPTP+SS, MPTPadministrated group with Sihosogan-tang; MPTP+SC, MPTPadministrated group with Sihocheonggan-tang. Scale bar, 200 Ìm. Data presented as means±standard deviation. Means with different letters are significantly different (p<0.05) by Duncan's multiple-range test.
그림 Fig. 5. Comparision of the protective effect of Sihogyegi-tang, Sihosogan-tang, and Sihocheonggan-tang on dopaminergic neurons in the striatum. The optical density of TH-positive neurons in the striatum. TH, tyrosine hydroxylase; NOR, saline-administrated group; MPTP, MPTP-administrated group; MPTP+SG, MPTP-administrated group with Sihogyegi-tang; MPTP+SS, MPTP-administrated group with Sihosogan-tang; MPTP+SC, MPTP-administrated group with Sihocheonggan-tang. Scale bar, 200 Ìm. Means with different letters are significantly different (p<0.05) by Duncan's multiplerange test.

참고문헌 (21)

Dauer W, Przedborski S. Parkinson's disease: mechanisms and models. Neuron. 2003 ; 39(6) : 889-909. https://doi.org/10. 1016/s0896-6273(03)00568-3

상세보기
Luquin MR, Kulisevsky J, Martinez-Martin P, Mir P, Tolosa ES. Consensus on the definition of advanced Parkinson's disease: A neurologists-based delphi study (CEPA study). Parkinsons Dis. 2017 ; 2017 : 4047392. https://doi.org/10.1155/2017/4047392

상세보기
Lee JE, Choi J, Lim HS, Kim JH, Cho JH, Kim GS, et al. The prevalence and incidence of Parkinson' s disease in South Korea: A 10-year nationwide population-based study. J Korean Neurol Assoc. 2017 ; 35(4) : 191-8. https://doi.org/10.17340/jkna.2017.4.1

상세보기
Breydo L, Wu JW, Uversky VN. α-Synuclein misfolding and Parkinson's disease. Biochim Biophys Acta. 2012 ; 1822(2) : 261-85. https://doi.org/10.1016/j.bbadis.2011.10.002

상세보기
Dodson MW, Guo M. Pink1, Parkin, DJ-1 and mitochondrial dysfunction in Parkinson's disease. Curr Opin Neurobiol. 2007 ; 17(3) : 331-7. https://doi.org/10.1016/j.conb.2007.04.010

상세보기
Olanow CW, Watts RL, Koller WC. An algorithm (decision tree) for the management of Parkinson's disease (2001): treatment guidelines. Neurology. 2001 ; 56(11 Suppl 5) : S1-88. https://doi.org/10.1212/wnl.56.suppl_5.s1

상세보기
Seo JE, Lee H, Bae CH, Yoon DH, Kim HY, Kim S. Comparative study of 12 herbal formulae covered by the national health insurance service in Korea. Korean J Acupunct. 2022 ; 39(2) : 34-42. https://doi.org/10.14406/acu.2022.006

원문보기 상세보기
Lee SH, Heo YJ, Cho YS. 4 cases of chronic abdominal pain children treated by Sihogyeji-tanggagam with acupuncture and moxibustion treatment. JPPKM. 2021 ; 35(6) : 261-6. https://doi.org/10.15188/kjopp.2021.12.35.6.261

원문보기 상세보기
Ko SJ, Kim JS, Park JW, Ha NY. Korean medicine clinical practice guideline for functional dyspepsia. 1st ed. Gyeongsan : Division of clinical practice guideline of Korean medicine in National Institute for Korean Medicine Development. 2021 : 100. Available from: URL:https://nikom.or.kr/nckm/module/practiceGuide/view.do?guide_idx192&menu_idx14
An JH, Hwang ML, Yoon HJ, Jung HA. A Review on the Sihocheonggan-tang & Sihocheonggan-san for the treatablity of atopic dermatitis. The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology. 2017 ; 30(1) : 10-6. https://doi.org/10.6114/JKOOD.2017.30.1.010

원문보기 상세보기
Kim BR, Huh K, Lim SW. Effect of Sihogeji-tang extract on indomethacin-Induced Gastric mucosal Lesions in Rats. Korean J Orient Int Med. 2010 ; 31(3) : 477-87.
Sun Y, Xu X, Zhang J, Chen Y. Treatment of depression with Chai Hu Shu Gan San: A systematic review and meta-analysis of 42 randomized controlled trials. BMC Complement Altern Med. 2018 ; 18(1) : 66. https://doi.org/10.1186/s12906-018-2130-z

상세보기
Li Z, Zeng Q, Hu S, Liu Z, Wang S, Jin Y, et al. Chaihu Shugan San ameliorated cognitive deficits through regulating gut microbiota in senescence-accelerated mouse prone 8. Front Pharmacol. 2023 ; 14 : 1181226. https://doi.org/10.3389/fphar.2023.1181226

상세보기
Food and Drug Administration. Estimating the maximum safe starting dose in initial clinical trials for therapeutics in adult healthy volunteers. Rockville : U.S. Food and Drug Administration. 2005 : 6-9. Available from: URL:https://www.fda.gov/regulatory-information/search-fda-guidance-documents/estimating-maximum-safe-starting-dose-initial-clinical-trials-therapeutics-adult-healthy-volunteers
Buettner GR. Superoxide dismutase in redox biology: the roles of superoxide and hydrogen peroxide. Anticancer Agents Med Chem. 2011 ; 11(4) : 341-6. https://doi.org/10.2174/1871520 11795677544

상세보기
Abe K, Taguchi K, Wasai T, Ren J, Utsunomia I, Shinohara T, et al. Biochemical and pathological study of endogenous 1-benzyl-1,2,3,4-tetrahydroisoquinoline-induced parkinsonism in the mouse. Brain Res. 2001 ; 907(1-2) : 134-8. https://doi.org/10.1016/s0006-8993(01)02573-2

상세보기
Park G, Park YJ, Yang HO, Oh MS. Ropinirole protects against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity in mice via anti-apoptotic mechanism. Pharmacol Biochem Behav. 2013 ; 104 : 163-8. https://doi.org/10.1016/j.pbb.2013.01.017

상세보기
Kang JM, Park HJ, Choi YG, Choe IH, Park JH, Kim YS, et al. Acupuncture inhibits microglial activation and inflammatory events in the MPTP-induced mouse model. Brain Res. 2007 ; 1131(1) : 211-9. https://doi.org/10.1016/j.brainres.2006.10.089

상세보기
Kum WF, Durairajan SSK, Bian ZX, Man SC, Lam YC, Xie LX, et al. Treatment of idiopathic Parkinson's disease with traditional Chinese herbal medicine: a randomized placebo-controlled pilot clinical study. Evid Based Complement Alternat Med. 2011 ; 2011 : 724353. https://doi.org/10.1093/ecam/nep116

상세보기
Elyasi L, Ghazvini H. The protective effects of Citrus aurantium extract on a 6-Hydroxydopamine-induced model of Parkinson's disease in male rats. Anatomical Sciences. 2020 ; 17(1) : 1-6.
Lee CH, Hwang DS, Kim HG, Oh H, Park H, Cho JH, et al. Protective effect of Cyperi rhizoma against 6-hydroxydopamine-induced neuronal damage. J Med Food. 2010 ; 13(3) : 564-71. https://doi.org/10.1089/jmf.2009.1252

원문보기 상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format