[논문]일반메밀과 쓴메밀의 새싹 추출물의 장내 유익균 증식 및 염증조절 효능 평가

김수정; 손황배; 김종원; 임상현; 이종남; 박수형; 남정환; 김도연; 이예진; 장동칠; 김율호

doi:10.7732/kjpr.2023.36.5.455

일반메밀과 쓴메밀의 새싹 추출물의 장내 유익균 증식 및 염증조절 효능 평가
Growth of Intestinal Bacteria and Intestinal Inflammation of Sprout Extract from Common Buckwheat and Tartary Buckwheat 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.36 no.5, 2023년, pp.455 - 468

초록
AI-Helper

본 연구에서는 메밀 새싹 추출물의 비피더스균과 락토바실러스균 증식에 미치는 영향, 항염증 효과 및 관련 생리활성 물질의 분석을 통해 프리바이오틱스 소재로서의 활용 가능성을 평가하였다. 메밀 새싹 추출물을 10종의 프로바이오틱스에 첨가하여 각각 배양하였을 때, 대조군보다 pH 값이 낮아지고, 생균수는 증가하였다. 쓴메밀 새싹 추출물을 Bifidobacterium longum ssp. infantis BT1에 첨가하여 배양한 실험군의 생균수는 8.61×10⁸ CFU/mL로 증식 효과가 가장 뛰어났다. 메밀 새싹 추출물을 10종의 프로바이오틱스에 첨가한 다음 LPS로 유도하였을 때, 세포독성에 대한 보호 효과와 NO, PGE₂ 발현에서도 유의적인 억제 효과를 보여 우수하였다. 특히, 비피더스균 중 BT1에서 NO 생성량이 29.5 mM로 가장 낮았으며, 락토바실러스균 중에서는 Lacticaseibacillus paracasei LPC5에서 30.6 mM로 NO 생성량이 매우 낮아 항염증 효과가 있었다. 이상의 결과는 루틴, 퀘세틴 및 콜린 등 다양한 생리활성 물질을 가진 메밀 새싹 추출물이 특정 배양액에서 염증 관련 지표인 NO 생성 및 PGE₂ 생성량 억제에 효과가 있음을 나타내는 것으로 메밀이 장내 유익균 증식과 염증 조절에 긍정적인 영향을 줄 수 있을 것으로 판단된다.

Abstract ▼ AI-Helper

We aimed to assess the potential growth-promoting effects of buckwheat sprout on intestinal bacteria and their anti-inflammation effects in a cellular model of intestinal inflammation. The growth of Bifidobacterium longum ssp. infantis BT1 was enhanced with the addition of the sprout extract of tartary buckwheat. Further, in the inflammatory model cells cultured with Raw 264.7 cells were treated with buckwheat sprout including each 10 probiotics before the addition of lipopolysaccharide (LPS) to induce inflammation in Raw 264.7 cells. Buckwheat sprout in both Bifidobacterium longum ssp. infantis BT1 and Lacticaseibacillus paracasei LPC5 significantly reduced the production of NO and PGE2. The above results indicate that buckwheat sprout extract which contains with various physiologically active substances such as rutin, quercetin, and choline is effective in suppressing NO and PGE2 production, which are inflammation-related indicators. The present study suggests that buckwheat sprout could induce positive effects on the intestinal beneficial bacteria and in anti-inflammation.

주제어

표/그림 (10)

그림 Fig. 1. Scheme for probiotic with buckwheat sprout on intestinal bacteria and their anti-inflammatory effects. Raw 264.7 cells were divided into 3 groups: CON, SCB and STB. CON, control medium without glucose group; SCB, Medium with sprout of common buckwheat (Fagopyrum esculentum Moench) with alcohol extract group; STB, Medium with sprout of tartary buckwheat (Fagopyrum tataricum Gaertn.) with alcohol extract group.
표 Table 1. Probiotics species used in this study and their origin
표 Table 2. The composition of different MRS and BL medium for the probiotics cultivation
그림 Fig. 2. pH change of 10 probiotics without/with buckwheat sprout extract by incubation time. SCB, Sprout extract of common buckwheat; STB, Sprout extract of tartary buckwheat; CON, Control without glucose medium. (A), Bifidobacterium longum ssp. infantis BT1; (B), Bifidobacterium longum BG7; (C), Bifidobacterium animal subsp. lactis BL3; (D), Bifidobacterium breve BR3; (E), Lactobacillus acidophilus LA1; (F), Lacticaseibacillus casei LC5; (G), Lactobacillus helveticus LH5; (H), Lactiplantibacillus plantarum LP3; (I), Lacticaseibacillus paracasei LPC5; (J), Lacticaseibacillus rhamnosus LR5.
그림 Fig. 3. OD change of 10 probiotics without/with buckwheat sprout extract by incubation time. SCB, Sprout extract of common buckwheat; STB, Sprout extract of tartary buckwheat; CON, Control without glucose medium. (A), Bifidobacterium longum ssp. infantis BT1; (B), Bifidobacterium longum BG7; (C), Bifidobacterium animal subsp. lactis BL3; (D), Bifidobacterium breve BR3; (E), Lactobacillus acidophilus LA1; (F), Lacticaseibacillus casei LC5; (G), Lactobacillus helveticus LH5; (H), Lactiplantibacillus plantarum LP3; (I), Lacticaseibacillus paracasei LPC5; (J), Lacticaseibacillus rhamnosus LR5.
그림 Fig. 4. Change of viable cell count of 10 probiotics without/with buckwheat sprout extract by incubation time. SCB, Sprout extract of common buckwheat; STB, Sprout extract of tartary buckwheat; CON, Control without glucose medium. (A), Bifidobacterium longum ssp. infantis BT1; (B), Bifidobacterium longum BG7; (C), Bifidobacterium animal subsp. lactis BL3; (D), Bifidobacterium breve BR3; (E), Lactobacillus acidophilus LA1; (F), Lacticaseibacillus casei LC5; (G), Lactobacillus helveticus LH5; (H), Lactiplantibacillus plantarum LP3; (I), Lacticaseibacillus paracasei LPC5; (J), Lacticaseibacillus rhamnosus LR5.
그림 Fig. 5. Nitric oxide production in the culture broth of Bifidobacterium animal subsp. lactis BL3 (A) and Lacticaseibacillus paracasei LPC5 (B) probiotics with buckwheat sprout extract by Raw 264.7 cells at 6, 24 and 48 hours. DMEM media was used for negative control (NC). LPS (100 ng/mL) was used for positive control (LPS). Data shown represent the mean ± SD based on three independent experiments. ***Represents a statistically significant difference of p < 0.001 compared with the negative control (NC).
그림 Fig. 6. Nitric oxide production in the culture broth of four Bifidobacterium (A) and six Lactobacillus (B) probiotics with buckwheat sprout extract by Raw 264.7 cells at 24 hours. DMEM media was used for negative control (NC). LPS (100 ng/mL) was used for positive control (LPS). Data shown represent the mean ± SD based on three independent experiments. ***Represents a statistically significant difference of p < 0.001 compared with the negative control (NC). BT1, Bifidobacterium longum ssp. infantis; BG7, Bifidobacterium longum; BL3, Bifidobacterium animal subsp. lactis; BR3, Bifidobacterium breve; LA1, Lactobacillus acidophilus; LC5, Lacticaseibacillus casei; LH5, Lactobacillus helveticus; LP3, Lactiplantibacillus plantarum; LPC5, Lacticaseibacillus paracasei; LR5, Lacticaseibacillus rhamnosus.
그림 Fig. 7. PGE₂ production in the culture broth of four Bifidobacterium (A) and six Lactobacillus (B) probiotics with buckwheat sprout extract by Raw 264.7 cells at 24 hours. DMEM media was used for negative control (NC). LPS (100 ng/mL) was used for positive control (LPS). Data shown represent the mean ±SD based on three independent experiments. ***Represents a statistically significant difference of p < 0.001 compared with the negative control (NC). BT1, Bifidobacterium longum ssp. infantis; BG7, Bifidobacterium longum; BL3, Bifidobacterium animal subsp. lactis; BR3, Bifidobacterium breve; LA1, Lactobacillus acidophilus; LC5, Lacticaseibacillus casei; LH5, Lactobacillus helveticus; LP3, Lactiplantibacillus plantarum; LPC5, Lacticaseibacillus paracasei; LR5, Lacticaseibacillus rhamnosus.
표 Table 3. Rutin, quercetin and choline contents in fresh seed and vinegar extract from common buckwheat and tartary buckwheat

참고문헌 (31)

Bae, J.Y. 2012. The effect of fermented extracts of Portulaca？oleracea against Campylobacter jejuni. Korean J. Food Nutr.？25(2):291-298.
Bruce, S.J., P.A. Guy, S. Rezzi and A.B. Ross. 2010. Quantitative measurement of betaine and free choline in plasma,？cereals and cereal products by isotope dilution LC-MS/MS.？J. Agric. Food Chem. 58(4):2055-2061.

상세보기
Chang, D.R. and J.B. Kim. 2010. A case study on Pulmone？healthy life's 'Pulmone vegetable lactic acid bacteria' advertising campaign: creating a new category in a growth market.？Korean J. Advertising 21:253-265.
Choi, J.Y. 2013. Protective effects of tartary buckwheat from？recognition impairment through anti-inflammation and protection from neuronal cell. Department of Food Science and？Nutrition, MS Thesis, Pusan National Univ., Korea. pp. 7-90.
Chow, J. 2002. Probiotics and prebiotics: a brief overview. J.？Ren. Nutr. 12:76-86.
DuPont, A.W. and H.L. DuPont. 2011. The intestinal microbiota？and chronic disorders of the gut. Nat. Rev. Gastroenterol.？Hepatol. 8:523-531.
Fooks, L.J. and R.G. Gibson. 2002. In vitro investigations of the？effect of probiotics and prebiotics on selected human intestinal？pathogens, FEMS Microbiol. Ecology 39(1):67-75.
Ganeshpurkar, A. and A.K. Saluja. 2017. The pharmacological？potential of rutin. Saudi Pharm. J. 25:149-164.
Gullo？n, B., T.A. Lu-Chau, M.T. Moreira, J.M. Lema and G.？Eibes. 2017. Rutin: a review on extraction, identification and？purification methods, biological activities and approaches to？enhance its bioavailability. Trends Food Sci. Technol. 67:220-235.

상세보기
Heselmans, M., G. Reid, L.M. Akkermans, H. Savelkoul, H.？Timmerman and F.M. Rombouts. 2005. Gut flora in health？and disease: potential role of probiotics. Curr. Issues Intest.？Microbiol. 6:1-7.

상세보기
Hwang, E.J., S.Y. Lee, S.J. Kwon, M.H. Park and H.O. Boo. 2006. Antioxidative, antimicrobial and cytotoxic activities？of Fagopyrum esculentum Moench extract in germinated？seeds. J. Korean Medicinal Crop Sci. 4:1-7.
Jeon, A.Y., K.H. Kim, S.J. Kwon, S.K. Roy, S.W. Cho and S.H.？Woo. 2015. Effects of LED light conditions on growth and？analysis of functional components in buckwheat sprout.？Korean J. Crop Sci. 60(3):388-393.
Karimi, O. and A.S. Peria. 2008. Indications and challenges of？probiotics, prebiotics, and synbiotics in the management of？arthralgias and spondyloarthropathies in inflammatory bowel？disease. J. Clin. Gastroenterol. 42:136S-141S.
Kim, S.J. and Y.H. Kim. 2018. Agricultural guide buckwheat.？Rural Development Administration, Jeonju, Korea. pp. 7-90.
Kim, S.J., H.B. Sohn, B. Kwon, M.S. Park, S.Y. Hong, J.H.？Nam, J.T. Suh, J.N. Lee, K.D. Kim, D.C. Chang and Y.H.？Kim. 2020. Potential prebiotic effects of yacon extract, a？source of fructooligosaccharides, on Bifidobacterium strains.？Korean J. Food Sci. Technol. 52(2): 149-155.
Kim, S.J., H.B. Sohn, G.H. Kim, Y.Y. Lee, S.Y. Hong, K.D.？Kim, J.H. Nam, D.C. Chang, J.T. Suh, B.J. Koo and Y.H.？Kim. 2017. Comparison and validation of rutin and quercetin？contents according to the extraction method of tartary buckwheat (Fagopyrum tataricum Gaertn.) Korean J. Food Sci.？Technol. 49:258-264.
Kim, S.J., H.B. Sohn, J.H. Nam, J.N. Lee, J.T. Suh, D.C. Chang？and Y.H. Kim. 2022a. Selection and application of pollinating？insects to improve seed production of buckwheat in net？house. Korean J. Plant Res. 35(1):10-22.
Kim, S.J., H.B. Sohn, J.M. Choi, E.J. Cho, J.H. Nam, J.N. Lee,？J.T. Suh, D.C. Chang and Y.H. Kim. 2022b. Anti-diabetic？effects of common buckwheat and tartary buckwheat in type？II diabetes animal model. Korean J. Food Sci. Technol.？54(1):17-27.
Kim, S.J., H.B. Sohn, K.T. Lee, J.S. Shin, S. Kim, J.H. Nam,？S.Y. Hong, J.T. Suh, D.C. Chang and Y.H. Kim. 2019.？Anti-inflammatory effects of seed ethanolic extracts of the？common buckwheat and tartary buckwheat are mediated？through the suppression of inducible nitric oxide synthase？and pro-inflammatory cytokines in LPS-induced Raw 264.7？macrophage cells. Korean J. Food Sci. Technol. 51(6):565-575.
Kim, S.J., O.K. Kwon, S.Y. Hong, J.W. Nam, H.W. Sohn, Y.I.？Jin, D.C. Chang and M.J. Cho. 2013. Characteristics and？effects of fructooligosaccharides in yacon (Samallanthus？sonchifolius). Korean J. Intl. Agri. 25:284-289.

상세보기
Kwon, C., J.W. Kim, Y.K. Park, S. Kang, M.J. Chung, S.J. Kim？and S. Lim. 2023. Bioconversion of rutin in tartary buckwheat by the Korean indigenous probiotics. Microbiol. Biotechnol. Lett. 51(1):1-10.
Lee, M.R., K.A. Lee, and S.Y. Lee. 2003. Improving effects of？fructooligosaccharides and isomalto-oligosaccharides contained？in sponge cakes on the constipation of female college students.？Korean J. Food Sci. Technol. 32:621-626.
Marshall, H.G., Y. Pomeranz and G. Chapter. 1982. Buckwheat？description, breeding, production and utilization. In Pomeranz,？Y. (ed.), Advanced in Cereal and Technology, Vol. V. Am.？Ass. Cereal Chem. Incorporated, St. Paul, MN (USA). pp.？157-210.
Niu, D., S. An, X. Chen, H. Bi, Q. Zhang, T. Wang, B. Han, H.？Zhang and J. Kang. 2020. Corni fructus as a natural resource？can treat type 2 diabetes by regulating gut microbiota. Am. J.？Chin. Med. 48(6):1385-1407.
Nuryana, I., A. Andriani, P. Lisdiyanti and Yopi. 2019. Analysis？of organic acids produced by lactic acid bacteria. IOP Conf.？Ser.: Earth Environ. Sci. 251:012054.

상세보기
Oh, N.S. and M.J. In. 2008. Production of a fermented soymilk？using a new strain Leuconostoc mesenteroides Kc51 isolated？from Kimchi. J. Korean Soc. Appl. Biol. Chem. 51(1):88-91.
Phillips, M.M. 2012. Analytical approaches to determination of？total choline in foods and dietary supplements. Anal. Bioanal.？Chem. 403:2103-2112.
Sung, M.K, S.J. Han, H.J. Seo, S.W. Choi, S.H. Nam and J.I.？Chung. 2014. Genotype and environment influence on raffinose？and stachyose content of soybean seed. Korean Soc. Crop？Sci. 59(3):319-324.
USDA Food Composition Databases (USDA). 2018. Nutrient？Lists. USDA National Nutrient Database-Choline USDA.？National Nutrient Database for Standard Reference Legacy？online.
Yun, J.Y, S.Y. Choi, P.J. Park, H.G. Chung, H.M. Shin, K.H.？Suk and B.O. Lim. 2008. Extracts of Artemisia princeps？Pampanini inhibit lipopolysaccharide-induced nitric oxide,？cyclooxygenase-2, prostaglandin E2, and tumor necrosis？factor-α, production from murine macrophage Raw 264.7？Cells. Kor. J. Medi. Crop. Sci. 16:326-331.
Zeisel, S.H. and J.K. Blusztajn. 1994. Choline and human？nutrition. Annu. Rev. Nutr. 14:269-296.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증