[논문]Chlorothalonil이 미역(Undaria pinnatifida) 배우체의 생존 및 상대성장률에 미치는 영향

박윤호; 심보람; 황운기; 이주욱

doi:10.11626/kjeb.2023.41.3.256

[국내논문] Chlorothalonil이 미역(Undaria pinnatifida) 배우체의 생존 및 상대성장률에 미치는 영향
The impact of chlorothalonil on female gametophyte survival rate and relative growth rate of Undaria pinnatifida 원문보기

환경생물 = Korean journal of environmental biology, v.41 no.3, 2023년, pp.256 - 265

박윤호 (국립환경과학원 환경건강연구부 위해성평가연구과) , 심보람 (국립수산과학원 서해수산연구소 기후환경자원과) , 황운기 (국립수산과학원 기후환경연구부 갯벌연구센터) , 이주욱 (국립수산과학원 서해수산연구소 기후환경자원과)

초록
AI-Helper

TBT 사용이 금지된 이후, 방오도료의 방오능력을 보완하기 위하여 booster biocides를 추가하여 사용하고 있다. Booster biocides 중에서 chlorothalonil은 해양환경 내 지속적으로 유입되며 다양한 해양생물에게 심각한 독성영향을 미치고 있지만 해조류에 대한 연구는 제한적이 다. 따라서 우리나라의 주요 양식생물인 미역(Undaria pinnatifida)의 초기생활사에 chlorothalonil이 미치는 영향을 분석하였다. U. pinnatifida의 암배우체를 chlorothalonil(0, 0.03, 0.05, 0.10, 0.20, 0.40 mg L^-1)에 노출하여 생존율과 상대성장률을 분석하였다. 암배우체 생존율의 무영향 농도(NOEC), 최소영향농도(LOEC), 반수치사농도(LC₅₀)는 0.05, 0.10, 0.141 (0.121~0.166) mg L^-1이었고 상대성장률의 NOEC, LOEC, 반수영향농도(EC₅₀)은 0.10, 0.20, 0.124 (0.119~0.131) mg L^-1로 분석되었다. 따라서, 미역의 암배우체는 0.05~0.10 mg L^-1 이상의 농도에서 독성영향을 받기 시작할 것으로 판단된다. 본 연구의 결과는 chlorothalonil에 대한 U. pinnatifida 초기생활사의 건강도를 평가하기 위한 기준자료로 활용될 것으로 기대된다.

Abstract ▼ AI-Helper

Chlorothalonil is continuously introduced into the marine environment and has significant toxic effects on various marine organisms, however, research on its effect on seaweed is limited. Therefore, we analyzed the impact of chlorothalonil on the early life stages of major aquaculture species in Korea, Undaria pinnatifida. U. pinnatifida female gametophytes were exposed to different concentrations of chlorothalonil (0, 0.03, 0.05, 0.10, 0.20, and 0.40 mg L-1), and their survival rate and relative growth rate were analyzed. The no observed effect concentration (NOEC), lowest observed effect concentration (LOEC), and median lethal concentration (LC50) for female gametophyte survival were determined as 0.05, 0.10, and 0.141(0.121-0.166)mg L-1. NOEC, LOEC, and median effective concentration (EC50) for relative growth rate were 0.10, 0.20, and 0.124 (0.119-0.131) mg L-1. Therefore, female gametophytes of U. pinnatifida are expected to experience toxic effects at concentrations above 0.05-0.10 mg L-1 of chlorothalonil. These research findings are anticipated to serve as crucial reference data for evaluating the effects of chlorothalonil on the health of U. pinnatifida in the early life stages.

Keyword

참고문헌 (40)

Al-Dulaimi O, ME Rateb, AS Hursthouse, G Thomson and M？Yaseen. 2021. The brown seaweeds of Scotland, their importance and applications. Environments 8:59. https://doi.org/10.3390/environments8060059

상세보기
Amara I, W Miled, RB Slama and N Ladhari. 2018. Antifouling？processes and toxicity effects of antifouling paints on marine？environment. A review. Environ. Toxicol. Pharmacol. 57:115-130. https://doi.org/10.1016/j.etap.2017.12.001

상세보기
Bao VWW, KMY Leung, JW Qiu and MHW Lam. 2011. Acute toxicities of five commonly used antifouling booster biocides to？selected subtropical and cosmopolitan marine species. Mar.？Pollut. Bull. 62:1147-1151. https://doi.org/10.1016/j.marpolbul.2011.02.041

상세보기
Bellas J. 2006. Comparative toxicity of alternative antifouling？biocides on embryos and larvae of marine invertebrates. Sci.？Total Environ. 367:573-585. https://doi.org/10.1016/j.scitotenv.2006.01.028

상세보기
Christen V, J Krebs and K Fent. 2019. Fungicides chlorothanolin,？azoxystrobin and folpet induce transcriptional alterations in？genes encoding enzymes involved in oxidative phosphorylation and metabolism in honey bees(Apis mellifera) at sublethal？concentrations. J. Hazard. Mater. 377:215-226. https://doi.org/10.1016/j.jhazmat.2019.05.056

상세보기
Cima F, M Bragadrin and L Ballarin. 2008. Toxic effects of new？antifouling compounds on tunicate haemocytes I. Sea-Nine？211TM and chlorothalonil. Aquat. Toxicol. 86:299-312. https://doi.org/10.1016/j.aquatox.2007.11.010

상세보기
DeLorenzo ME and MH Fulton. 2012. Comparative risk assessment of permethrin, chlorothalonil, and diuron to coastal？aquatic species. Mar. Pollut. Bull. 64:1291-1299. https://doi.org/10.1016/j.marpolbul.2012.05.011

상세보기
DeLorenzo ME and L Serrano. 2003. Individual and mixture toxicity of three pesticides; atrazine, chlorpyrifos, and chlorothalonil？to the marine phytoplankton species Dunaliella tertiolecta.？J. Environ. Sci. Health Part B-Pestic. Contam. Agric. Wastes？38:529-538. https://doi.org/10.1081/PFC-120023511

상세보기
Ernst W, K Doe, P Jonah, J Young, G Julien and P Hennigar. 1991.？The toxicity of chlorothalonil to aquatic fauna and the impact？of its operational use on a pond ecosystem. Arch. Environ.？Contam. Toxicol. 21:1-9. https://doi.org/10.1007/BF01055550

상세보기
Garaventa F, C Gambardella, A Di Fino, M Pittore and M Faimali. 2010. Swimming speed alteration of Artemia sp. and Brachionus plicatilis as a sub-lethal behavioural end-point for？ecotoxicological surveys. Ecotoxicology 19:512-519. https://doi.org/10.1007/s10646-010-0461-8

상세보기
Guerreiro ADS, FEL Abreu, G Fillmann and JZ Sandrini. 2020.？Effects of chlorothalonil on the antioxidant defense system？of mussels Perna perna. Ecotox. Environ. Safe. 190:110119.？https://doi.org/10.1016/j.ecoenv.2019.110119

상세보기
Guerreiro ADS, RC Rola, MT Rovani, SRD Costa and JZ Sandrini. 2017. Antifouling biocides: Impairment of bivalve immune system by chlorothalonil. Aquat. Toxicol. 189:194-199. https://doi.org/10.1016/j.aquatox.2017.06.012

상세보기
Hamer M, SK Maynard and S Schneider. 2019. A pulsed-dose？study evaluating chronic toxicity of chlorothalonil to fish: A case？study for environmental risk assessment. Environ. Toxicol.？Chem. 38:1549-1559. https://doi.org/10.1002/etc.4421

상세보기
Haque MN, HJ Eom, SE Nam, YK Shin and JS Rhee. 2019. Chlorothalonil induces oxidative stress and reduces enzymatic？activities of Na + /K + -ATPase and acetylcholinesterase in gill？tissues of marine bivalves. PLoS One 14:e0214236. https://doi.org/10.1371/journal.pone.0214236

상세보기
Heo S, JW Lee, H Choi, SJ Yoon, KY Kwon, UK Hwang and YH？Park. 2021. Toxic effect of chlorothalonil, an antifouling agent,？on survival and population growth rate of a marine rotifer, Brachionus plicatilis. Korean J. Environ. Biol. 39:390-398. https://doi.org/10.11626/KJEB.2021.39.3.390

원문보기 상세보기
Hintze S, YSB Hannalla, S Guinchard, D Hunkeler and G Glauser. 2021. Determination of chlorothalonil metabolites in soil and？water samples. J. Chromatogr. A 1655:462507. https://doi.org/10.1016/j.chroma.2021.462507

상세보기
Johansson P, KM Eriksson, L Axelsson and H Blanck. 2012. Effects of seven antifouling compounds on photosynthesis and？inorganic carbon use in sugar kelp Saccharina latissima (Linnaeus). Arch. Environ. Contam. Toxicol. 63:365-377. https://doi.org/10.1007/s00244-012-9778-z

상세보기
Jung SM, JS Bae, SG Kang, JS Son, JH Jeon, HJ Lee, JY Jeon,？M Sidharthan, SH Ryu and HW Shin. 2017. Acute toxicity？of organic antifouling biocides to phytoplankton Nitzschia？pungens and zooplankton Artemia larvae. Mar. Pollut. Bull.？124:811-818. https://doi.org/10.1016/j.marpolbul.2016.11.047

상세보기
Key PB, SL Meyer and KW Chung. 2003. Lethal and sub-lethal？effects of the fungicide chlorothalonil on three life stages of？the grass shrimp, Palaemonetes pugio. J. Environ. Sci. Health？Part B-Pestic. Contam. Agric. Wastes 38:539-549. https://doi.org/10.1081/PFC-120023512

상세보기
Lee H, J Park, K Shin, S Depuydt, S Choi, J de Saeger and T Han.？2020a. Application of a programmed semi-automated Ulva？pertusa bioassay for testing single toxicants and stream water？quality. Aquat. Toxicol. 221:105426. https://doi.org/10.1016/j.aquatox.2020.105426

상세보기
Lee H, S Depuydt, S Choi, T Han and J Park. 2020b. Rapid toxicity assessment of six antifouling booster biocides using a？microplate-based chlorophyll fluorescence in Undaria pinnatifida gametophytes. Ecotoxicology 29:559-570. https://doi.org/10.1007/s10646-020-02207-2

상세보기
Lee JW, H Choi, YH Park, Y Lee, S Heo and UK Hwang. 2019. Toxic evaluation of phenanthrene and zinc undecylenate using the population growth rates of marine diatom, Skeletonema？costatum. Korean J. Environ. Biol. 37:372-379. https://doi.org/10.11626/KJEB.2019.37.3.372

원문보기 상세보기
Lee JW, YH Park, BR Sim, HJ Jeon, S Heo and UK Hwang. 2022.？A study of environmental conditions of survival rate and relative growth rate in female gametophyte of Undaria pinnatifida？for toxicity assessment. J. Mar. Life Sci. 7:86-93. https://doi.org/10.23005/ksmls.2022.7.2.86

원문보기 상세보기
Lee MRN, UJ Kim, IS Lee, M Choi and JE Oh. 2015. Assessment？of organotin and tin-free antifouling paints contamination in？the Korean coastal area. Mar. Pollut. Bull. 99:157-165. https://doi.org/10.1016/j.marpolbul.2015.07.038

상세보기
Lee S, J Chung, H Won, D Lee and YW Lee. 2011. Analysis of？antifouling agents after regulation of tributyltin compounds in？Korea. J. Hazard. Mater. 185:1318-1325. https://doi.org/10.1016/j.jhazmat.2010.10.048

상세보기
Li X, Y Yao, S Wang and S Xu. 2020. Resveratrol relieves chlorothalonil-induced apoptosis and necroptosis through miR-15a/？Bcl2-A20 axis in fish kidney cells. Fish Shellfish Immunol.？107:427-434. https://doi.org/10.1016/j.fsi.2020.11.007

상세보기
Lin H, S Zhao, X Fan, Y Ma, X Wu, Y Su and J Hu. 2019. Residue？behavior and dietary risk assessment of chlorothalonil and its？metabolite SDS-3701 in water spinach to propose maximum？residue limit (MRL). Regul. Toxicol. Pharmacol. 107:104416.？https://doi.org/10.1016/j.yrtph.2019.104416

상세보기
Lopes FC, ASV Junior, CD Corcini, JAA Sanchez, DM Pires, JR？Pereira, EG Primel, G Fillmannm and CDMG Martins. 2020.？Impacts of the biocide chlorothalonil on biomarkers of oxidative stress, genotoxicity, and sperm quality in guppy Poecilia？vivipara. Ecotox. Environ. Safe. 188:109847. https://doi.org/10.1016/j.ecoenv.2019.109847

상세보기
MOF. 2018. Korean Standard Method of Examination for Marine？Environment. Ministry of Oceans and Fisheries. Sejong,？Korea. https://www.law.go.kr/LSW/admRulInfoP.do?admRulSeq2100000170850#J6-0:0. Accessed August 29, 2023.
Morais LG, PK Gusso-Choueri, FEL Abreu, IB Castro, DM Abessa and RB Choueri. 2023. Multilevel assessment of chlorothalonil sediment toxicity to Latin American estuarine biota:？Effects on biomarkers, reproduction and survival in different？benthic organisms. Sci. Total Environ. 872:162215. https://doi.org/10.1016/j.scitotenv.2023.162215

상세보기
Onduka T, A Kakuno, K Kono, K Ito, K Mochida and K Fujii. 2012.？Toxicity of chlorothalonil to marine organisms. Fish. Sci.？78:1301-1308. https://doi.org/10.1007/s12562-012-0562-9

상세보기
Soroldoni S, F Abreu, IB Castro, FA Duarte and GLL Pinho. 2017.？Are antifouling paint particles a continuous source of toxic？chemicals to the marine environment? J. Hazard. Mater. 330:76-82. https://doi.org/10.1016/j.jhazmat.2017.02.001

상세보기
Tatewaki M. 1966. Formation of a crustose sporophyte with unilocular sporangia in Scitosiphon lomentaria. Phycologia 6:62-66. https://doi.org/10.2216/i0031-8884-6-1-62.1

상세보기
Thomas KV and S Brooks. 2010. The environmental fate and？effects of antifouling paint biocides. Biofouling 26:73-88.？https://doi.org/10.1080/08927010903216564

상세보기
Van Scoy AR and RS Tjeerdema. 2014. Environmental fate and？toxicology of chlorothalonil. Rev. Environ. Contam. Toxicol.？323:89-105. https://doi.org/10.1007/978-3-319-06746-9_4
Voulvoulis N, MD Scrimshaw and JN Lester. 2000. Occurrence？of four biocides utilized in antifouling paints, as alternatives to？organotin compounds, in waters and sediments of a commercial estuary in the UK. Mar. Pollut. Bull. 40:938-946. https://doi.org/10.1016/S0025-326X(00)00034-5

상세보기
Wyss GS, R Charudattan, EN Rosskopf and RC Littell. 2004.？Effects of selected pesticides and adjuvants on germination？and vegetative growth of Phomopsis amaranthicola, a biocontrol agent for Amaranthus spp. Weed Res. 44:469-482.？https://doi.org/10.1111/j.1365-3180.2004.00425.x

상세보기
Yee MSL, PS Khiew, WS Chiu, YF Tan, YY Kok and CO Leong. 2016. Green synthesis of graphene-silver nanocomposites？and its application as a potent marine antifouling agent.？Colloid Surf. B-Biointerfaces 148:392-401. https://doi.org/10.1016/j.colsurfb.2016.09.011

상세보기
Zhang M, Z Xu, Y Teng, P Christie, J Wang, W Ren, Y Luo and Z Li. 2016. Non-target effects of repeated chlorothalonil application？on soil nitrogen cycling: The key functional gene study. Sci.？Total Environ. 543:636-643. https://doi.org/10.1016/j.scitotenv.2015.11.053

상세보기
Zhang Q, M Saleem and C Wang. 2017. Probiotic strain Stenotrophomonas acidaminiphila BJ1 degrades and reduces？chlorothalonil toxicity to soil enzymes, microbial communities？and plant roots. AMB Express 7:227. https://doi.org/10.1186/s13568-017-0530-y

상세보기

저자의 다른 논문 :

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증