[논문]로스팅 담팔수 지상부 추출물 및 분획물의 성분 분석 및 항산화 활성

조양희; 최재혁; 김준일; 곽태일; 이우람

doi:10.15230/scsk.2024.50.1.49

로스팅 담팔수 지상부 추출물 및 분획물의 성분 분석 및 항산화 활성
Comparison of Ingredients and Antioxidant Activity of Roasted Aerial Parts of Elaeocarpus sylvestris Extracts and Fractions

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.50 no.1, 2024년, pp.49 - 58

조양희 ((주)팜스킨) , 최재혁 ((주)팜스킨) , 김준일 ((주)팜스킨) , 곽태일 ((주)팜스킨) , 이우람 ((주)팜스킨)

초록
AI-Helper

담팔수는 괭이밥목 담팔수과의 상록교목으로 추위에 약하여 아열대 지역에서만 자생하는 식물이다. 담팔수는 flavonoid, coumarin, polyphenol 성분이 보고되어져 있으며, 해당 성분들에 의하여 항균 및 항산화 효능들이 있는 것으로 보고되어져 있다. 본 연구에서는 담팔수의 생리활성을 높이기 위하여 로스팅 기법을 도입하였으며, 로스팅한 담팔수 추출물 및 분획물에 대한 성분 변화를 확인한 후 항산화, 총 페놀 및 총 플라보노이드 함량 측정을 진행하였다. 로스팅 전 후 추출물 분석을 진행하여 총 4 개 성분(brevifolin, ellagic acid, quercetin, kaempferol)의 함량이 증가되는 것을 확인하였으며, 로스팅을 한 추출물에서 보다 나은 항산화 효능을 나타내는 것을 확인하였다. 최적 로스팅 조건은 200 ℃에서 30 min 로스팅 처리를 하였을 때 항산화 효능이 가장 뛰어난 것으로 확인하였으며, 총 페놀 및 총 플라보노이드 함량 또한 가장 우수한 것으로 확인하였다. 최적 로스팅 조건으로 제조한 담팔수 추출물에서 향상된 항산화 효능을 확인하였으며, 향후 화장품 및 식품 소재로 활용이 가능할 것으로 사료된다.

Abstract ▼ AI-Helper

Elaeocarpus sylvestris var. ellipticus is an evergreen tree of the family Elaeocarpaceae, which is a plant that grows naturally only in subtropical regions due to its vulnerability to cold. E. sylvestris has been reported to have flavonoids, coumarins, and polyphenols, and it is reported that these components have antibacterial and antioxidant effects. In this study, a roasting technique was introduced to increase the physiological activity of E. sylvestris, and antioxidant, total phenol, and total flavonoid content were measured after confirming changes in the ingredients of roasted E. sylvestris extracts and fractions. We analyzed the extracts before and after roasting and found an increase in the content of four components (brevifolin, ellagic acid, quercetin, and kaempferol), with the roasted extracts showing better antioxidant activity. The optimal roasting condition was confirmed to have the best antioxidant effect when roasting at 200 ℃ for 30 min, and the total phenol and total flavonoid content were also confirmed to be the best. E. sylvestris extract produced under optimal roasting conditions has been confirmed to exhibit improved antioxidant effects, and it is believed that it can be used as a cosmetic and food material in the future.

주제어

표/그림 (10)

표 Table 1. Standard Compounds Contents of E. sylvestris According to Roasting
그림 Figure 1. (A) HPLC chromatogram of E. sylvestris aerial parts extract and (B) HPLC chromatogram of roasted E. sylvestris aerial parts extract; 1. brevifolin, 2. ellagic acid, 3. quercetin, 4. kaempferol.
그림 Figure 2. Antioxidant activity of roasting E. sylvestris according to temperature. (A) DPPH radical scavenging activity (% of control), (B) ABTS radical scavenging activity (% of control). All values are presented as mean ± SD (N = 3).
표 Table 2. Standard Compounds Contents, Total Phenolic Content and Total Flavonoid Content of Roasting E. sylvestris According to Temperature
그림 Figure 3. Antioxidant activity of roasting E. sylvestris according to time. (A) DPPH radical scavenging activity (% of control), (B) ABTS radical scavenging activity (% of control). All values are presented as mean ± SD (N = 3).
표 Table 3. Standard Compounds Contents, Total Phenolic Content and Total Flavonoid Content of Roasting E. sylvestris According to Time
$Figure 4. Chromatogram of roasted E. sylvestris aerial parts extract and fractions using HPLC analysis. (A) Roasted E. sylvestris aerial parts extract, (B) n-hexane fraction, (C) CH<sub>2</sub>Cl<sub>2</sub> fraction, (D) EtOAc fraction, (E) n-BuOH fraction and (F) DW fraction; 1. brevifolin, 2. ellagic acid, 3. quercetin, 4. kaempferol.$ 그림 Figure 4. Chromatogram of roasted E. sylvestris aerial parts extract and fractions using HPLC analysis. (A) Roasted E. sylvestris aerial parts extract, (B) n-hexane fraction, (C) CH₂Cl₂ fraction, (D) EtOAc fraction, (E) n-BuOH fraction and (F) DW fraction; 1. brevifolin, 2. ellagic acid, 3. quercetin, 4. kaempferol.
표 Table 4. Standard Compounds Contents in Extract and Fractions from Roasting E. sylvestris
표 Table 5. Antioxidative Activities of Extract and Fractions from Roasting E. sylvestris
표 Table 6. Total Phenolic and Flavonoid Contents in Extract and Fractions from Roasting E. sylvestris

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