[논문]Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing

Zowawi, Hosam M.; Alenazi, Thamer H.; AlOmaim, Waleed S.; Wazzan, Ahmad; Alsufayan, Abdullah; Hasanain, Rihab A.; Aldibasi, Omar S.; Althawadi, Sahar; Altamimi, Sarah A.; Mutabagani, Maysoon; Alamri, Maha; Almaghrabi, Reem S.; Al-Abdely, Hail M.; Memish, Ziad A.; Alqahtani, Saleh A.

doi:10.1128/jcm.03004-20

Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing 원문보기

Journal of clinical microbiology, v.59 no.5, 2021년, pp.e03004-20 - e03004-20

Zowawi, Hosam M. (College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia) , Alenazi, Thamer H. (College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia) , AlOmaim, Waleed S. (Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia) , Wazzan, Ahmad (College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia) , Alsufayan, Abdullah (Prince Sultan Military Medical City, Ministry of Defence, Riyadh, Saudi Arabia) , Hasanain, Rihab A. (IQVIA, Riyadh, Saudi Arabia) , Aldibasi, Omar S. (College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia) , Althawadi, Sahar (Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia) , Altamimi, Sarah A. (Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia) , Mutabagani, Maysoon (Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saud) , Alamri, Maha , Almaghrabi, Reem S. , Al-Abdely, Hail M. , Memish, Ziad A. , Alqahtani, Saleh A.

Abstract ▼ AI-Helper

Combating the ongoing coronavirus disease 2019 (COVID-19) pandemic demands accurate, rapid, and point-of-care testing with fast results to triage cases for isolation and treatment. The current testing relies on reverse transcriptase PCR (RT-PCR), which is routinely performed in well-equipped laboratories by trained professionals at specific locations.Combating the ongoing coronavirus disease 2019 (COVID-19) pandemic demands accurate, rapid, and point-of-care testing with fast results to triage cases for isolation and treatment. The current testing relies on reverse transcriptase PCR (RT-PCR), which is routinely performed in well-equipped laboratories by trained professionals at specific locations. However, during busy periods, high numbers of samples queued for testing can delay the test results, impacting efforts to reduce the infection risk. Besides, the absence of well-established laboratories at remote sites and low-resourced environments can contribute to a silent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). These reasons compel the need to accommodate point-of-care testing for COVID-19 that meets the ASSURED criteria (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable). This study assessed the agreement and accuracy of the portable Biomeme SARS-CoV-2 system against the gold standard tests. Nasopharyngeal and nasal swabs were used. Of the 192 samples tested using the Biomeme SARS-CoV-2 system, the results from 189 samples (98.4%) were in agreement with the reference standard-of-care RT-PCR testing for SARS-CoV-2. The portable system generated simultaneous results for nine samples in 80 min with high positive and negative percent agreements of 99.0% and 97.8%, respectively. We performed separate testing in a sealed glove box, offering complete biosafety containment. Thus, the Biomeme SARS-CoV-2 system can help decentralize COVID-19 testing and offer rapid test results for patients in remote and low-resourced settings.

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참고문헌 (38)

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing 원문보기

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