[논문]Insect Resistance in Transgenic Chrysanthemum [Dendranthema ×grandiflorum (Ramat.) Kitamura] by the Introduction of a Modified δ-endotoxin Gene of Bacillus thuringiensis

Shinoyama, Harue; Mochizuki, Atsushi; Komano, Masayasu; Nomura, Yukio; Nagai, Teruyuki

doi:10.1270/jsbbs.53.359

Insect Resistance in Transgenic Chrysanthemum [Dendranthema ×grandiflorum (Ramat.) Kitamura] by the Introduction of a Modified δ-endotoxin Gene of Bacillus thuringiensis 원문보기

Breeding science, v.53 no.4, 2003년, pp.359 - 367

Shinoyama, Harue (Fukui Agricultural Experiment Station) , Mochizuki, Atsushi (National Institute for Agro-Environmental Sciences) , Komano, Masayasu (Fukui Agricultural Experiment Station) , Nomura, Yukio (Fukui Agricultural Experiment Station) , Nagai, Teruyuki (Fukui Agricultural Experiment Station)

Abstract ▼ AI-Helper

We introduced a modified delta-endotoxin gene, modified cry1Ab (mcbt) of Bacillus thuringiensis, which displays a specific biological activity against lepidopteran insects into chrysanthemum [Dendranthema × grandiflorum (Ramat.) Kitamura]. The chrysanthemum cultivar ‘Shuho-no-chikara’ was transformed using a disarmed strain of Agrobacterium tumefaciens, EHA101, carrying a binary vector, pIG121mcbt that harboured a mcbt gene encoding an insecticidal crystal protein (ICP) fragment of B. thuringiensis var. kurstaki HD-1. Leaf discs were co-cultured with Agrobacterium and thereafter cultured on the callus induction medium containing G418. A total of 317 shoots were regenerated from 3,618 leaf discs on the regeneration medium (8.8 %). The mcbt gene was detected in all the regenerated plantlets by Southern blot analysis. The accumulation of Cry1Ab ICP in 20 transformed lines, selected at random, was confirmed by Western blot analysis. The level of accumulation of Cry1Ab ICP ranged from 10.5 ng to 80 ng per 50 μg total soluble protein (from 0.021 to 0.16 % of the total protein). Insect bioassay was conducted using tobacco budworm (Helicoverpa armigera) larvae and all the larvae of H. armigera tested died during the first instar on the leaves of the mcbt-transformed lines whose expression level of Cry1Ab ICP exceeded 47.6 ng per 50 μg compared to the wild type cry1Ab-transformed lines. Significantly higher feeding inhibition and/or growth inhibition of the insects was observed, compared to those on the non-transformed control plants.

주제어

참고문헌 (28)

Plant Mol. Biol. Adang, M.J., M.S. Brody, G. Cardineau, N. Eagan, R.T. Rouch, C.K. Shewmarker, A. Jonew, J.V. Oakes and K.E. McBride 21 1131 1993 10.1007/BF00023609

상세보기
Bradford, Marion M.. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical biochemistry, vol.72, no.1, 248-254.

상세보기
Chen, Chyi-Ying A., Shyu, Ann-Bin. AU-rich elements: characterization and importance in mRNA degradation. Trends in biochemical sciences, vol.20, no.11, 465-470.

상세보기
0305-1048 Nucleic Acids Res. Dean, C., S. Tamaki, P. Dunsmuir, M. Favreau, C. Katayama, H. Dooner and J. Bedbrook 14 2229 1986 10.1093/nar/14.5.2229

상세보기
0733-222X Bio/Technology 11 10 1151 1993 10.1038/nbt1093-1151

상세보기
0092-8674 Cell Goodall, G. and W. Filipowicz 58 3 473 1989 10.1016/0092-8674(89)90428-5

상세보기
Hiei, Yukoh, Ohta, Shozo, Komari, Toshihiko, Kumashiro, Takashi. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T‐DNA. The Plant journal : for cell and molecular biology, vol.6, no.2, 271-282.

상세보기
0014-2956 Eur. Biochem. Höfte, H., H. de Greve, J. Seurinck, S. Jansens, J. Mahillon, C. Ampe, J. Vandekerckhove, H. Vanderbruggen, M. van Montague, M. Zabeau and M. Vaeck 161 2 273 1986 10.1111/j.1432-1033.1986.tb10443.x

상세보기
0536-3683 Jpn. J. Breed. HONDA H 40 3 339 1990 10.1270/jsbbs1951.40.339

상세보기
0021-9193 J. Bacteriol. Hood, E.E., Helmer, G.L., Fraley, R.T., and Chilton, M.D. 168 3 1291 1986 10.1128/jb.168.3.1291-1301.1986

상세보기
0733-222X Bio/Technology 11 2 194 1993
0028-0836 Nature Laemmli, U.K. 227 680 1970 10.1038/227680a0

상세보기
0003-6862 Jpn. J. Appl. Zool. Mochizuki, A. 38 46 1994 10.1303/jjaez.38.46

상세보기
0031-9317 Physiol. Plant. Murashige, T. and F. Skoog 15 473 1962 10.1111/j.1399-3054.1962.tb08052.x

상세보기
Plant Mol. Biol. Murray, E.E., T. Rocheleau, M. Eberle, C. Stock, V. Sekar and M. Adang 16 1035 1991 10.1007/BF00016075

상세보기
0002-1369 Agric. Biol. Chem. Nagamatsu, Y., Y. Itai, C. Hatanaka, G. Funatsu and K. Hayashi 48 3 611 1984 10.1271/bbb1961.48.611

상세보기
0027-8424 Proc. Natl. Acad. Sci. USA 90 24 11811 1993 10.1073/pnas.90.24.11811

상세보기
0032-0781 Plant Cell Physiol. OHTA S 31 6 805 1990
0733-222X Bio/Technology 8 10 939 1990
0027-8424 Proc. Natl. Acad. Sci. USA 88 8 3324 1991 10.1073/pnas.88.8.3324

상세보기
0036-8075 Science Saiki, R.K., D. H. Gelfand, S. Stoffel, S.J. Scharf, R. Higuchi, G.T. Horn, K.B. Mullis and H.A. Erlich 487 1988
0092-8674 Cell Shaw, G. and Kamen, R. 46 5 659 1986 10.1016/0092-8674(86)90341-7

상세보기
Shinoyama, Harue, Komano, Masayasu, Nomura, Yukio, Nagai, Teruyuki. Introduction of Delta-endotoxin Gene of Bacillus thuringiensis to Chrysanthemum [Dendranthema × grandiflorum (Ramat.) Kitamura] for Insect Resistance. Breeding science, vol.52, no.1, 43-50.

상세보기
0022-2836 J. Mol. Biol. Southern, E.M. 98 503 1975 10.1016/S0022-2836(75)80083-0

상세보기
0962-8819 Transgenic Res. Sutton, D.W., P.K. Havstand and J.D. Kemp 1 228 1992 10.1007/BF02524753

상세보기
0536-3683 Jpn. J. Breed. 43 192 1993
Plant Mol. Biol. Van Aarssen, R., P. Soetaert, M. Stam, J. Dockx, V. Gossele, J. Seurinck, A. Reynaerts and M. Cornelissen 28 513 1995 10.1007/BF00020398

상세보기
Vaucheret, Hervé, Béclin, Christophe, Elmayan, Taline, Feuerbach, Frank, Godon, Christian, Morel, Jean‐Benoit, Mourrain, Philippe, Palauqui, Jean‐Christophe, Vernhettes, Samantha. Transgene‐induced gene silencing in plants. The Plant journal : for cell and molecular biology, vol.16, no.6, 651-659.

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Insect Resistance in Transgenic Chrysanthemum [Dendranthema ×grandiflorum (Ramat.) Kitamura] by the Introduction of a Modified δ-endotoxin Gene of Bacillus thuringiensis 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (28)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

연관된 기능

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트