[논문]Pollen Typhae total flavones inhibit expression of interleukin-6 in C2C12 skeletal muscle cells cultured with palmitate

Lou, SY

doi:10.3736/jcim20080511

Pollen Typhae total flavones inhibit expression of interleukin-6 in C2C12 skeletal muscle cells cultured with palmitate

中西醫結合學報 = Journal of Chinese integrative medicine, v.6 no.5, 2008년, pp.488 - 492

Lou, SY

Abstract ▼ AI-Helper

OBJECTIVE: To observe the effects of Pollen Typhae total flavones (PTF) on expression of interleukin-6 (IL-6) mRNA and protein secretion in C2C12 cell strain of skeletal muscle cells cultured with palmitate, and to explore the mechanism of PTF in relieving insulin resistance (IR). METHODS: The IR of C2C12 cells was induced by co-culturing with palmitate. The C2C12 cells were divided into normal control group, untreated group, PDTC (a nuclear factor-kappaB inhibitor) treated group, rosiglitazone (ROS)-treated group, ROS+ PDTC-treated group, PTF-treated group and PTF+PDTC-treated group. Sixteen hours after culture, the transportation rate of glucose was observed by (3)H-deoxyglucose uptake method; IL-6 mRNA expression in C2C12 cells was assayed by real time polymerase chain reaction (Real-time PCR), and level of IL-6 protein secretion in culture supernatant was detected by enzyme linked immunosorbent assay. RESULTS: Compared with the normal control group, the transportation rate of glucose of cells in untreated group was decreased 30.43% after 16-hour palmitate culture, and was increased 32.39% in the PTF-treated group. Compared with the untreated group, the levels of IL-6 mRNA expression in cells and IL-6 protein secretion in supernatant were significantly decreased in the PTF-treated group (P<0.05). The levels of IL-6 mRNA expression in cells and IL-6 protein secretion in supernatant were increased in PTF+PDTC-treated group as compared with PFT-treated group (P<0.05). CONCLUSION: PTF can inhibit the IL-6 mRNA expression and IL-6 protein secretion via nuclear factor-kappaB pathway in C2C12 skeletal muscle cells, which may be one of its mechanisms in relieving inflammation conditions and insulin resistance in C2C12 skeletal muscle cells.

참고문헌 (12)

Hirabara, Sandro M., Silveira, Leornardo R., Abdulkader, Fernando, Carvalho, Carla R.O., Procopio, Joaquim, Curi, Rui. Time-dependent effects of fatty acids on skeletal muscle metabolism. Journal of cellular physiology, vol.210, no.1, 7-15.

상세보기
de Luca, C., Olefsky, J.M.. Inflammation and insulin resistance. FEBS letters, vol.582, no.1, 97-105.

상세보기
Bruce, Clinton R., Dyck, David J.. Cytokine regulation of skeletal muscle fatty acid metabolism: effect of interleukin-6 and tumor necrosis factor-α. American journal of physiology, Endocrinology and metabolism, vol.287, no.4, E616-E621.

상세보기
0193-1849 Am. J. Physiol. Endocrinol. Metab. Kem 280 5 E745 2001 10.1152/ajpendo.2001.280.5.E745 Adipose tissue tumor necrosis factor and interleukin-6 expression in human obesity and insulin resistance

상세보기
Jové, Mireia, Planavila, Anna, Laguna, Juan Carlos, Vázquez-Carrera, Manuel. Palmitate-Induced Interleukin 6 Production Is Mediated by Protein Kinase C and Nuclear-Factor κB Activation and Leads to Glucose Transporter 4 Down-Regulation in Skeletal Muscle Cells. Endocrinology, vol.146, no.7, 3087-3095.

상세보기
Jové, Mireia, Planavila, Anna, Sánchez, Rosa M., Merlos, Manuel, Laguna, Juan Carlos, Vázquez-Carrera, Manuel. Palmitate Induces Tumor Necrosis Factor-α Expression in C2C12 Skeletal Muscle Cells by a Mechanism Involving Protein Kinase C and Nuclear Factor-κB Activation. Endocrinology, vol.147, no.1, 552-561.

상세보기
0742-4221 Diabetes Metab. Rev. Reaven 4 7 639 1988 10.1002/dmr.5610040703 Role of insulin in regulation of lipoprotein metabolism in diabetes

상세보기
Itani, S I, Zhou, Q, Pories, W J, MacDonald, K G, Dohm, G L. Involvement of protein kinase C in human skeletal muscle insulin resistance and obesity.. Diabetes, vol.49, no.8, 1353-1358.

상세보기
Pradhan, A D, Manson, J E, Rifai, N, Buring, J E, Ridker, P M. C-reactive protein, interleukin 6, and risk of developing type 2 diabetes mellitus.. JAMA : the journal of the American Medical Association, vol.286, no.3, 327-334.

상세보기
Jiangsu Zhong Yi YaoJiangsu Zhong Yi Yao Yan 26 1 59 2005 Advancement in research of preparation of Pollen Typhae and its chemical components and pharmacological effect
10.3736/jcim20060610
Jiujiang Yi Xue Zhu 17 4 218 2002 Clinical observation on effect of Pollen Typhae in treating 60 cases of hyperlipidemia

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Pollen Typhae total flavones inhibit expression of interleukin-6 in C2C12 skeletal muscle cells cultured with palmitate

Abstract ▼ AI-Helper

참고문헌 (12)

이 논문을 인용한 문헌

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트