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Nature communications, v.8 no.1, 2017년, pp.2024 -
Moreno-Mateos, Miguel A. (Department of Genetics, Yale University School of Medicine, New Haven, CT 06510 USA) , Fernandez, Juan P. (Department of Genetics, Yale University School of Medicine, New Haven, CT 06510 USA) , Rouet, Romain (Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 USA) , Vejnar, Charles E. (Department of Genetics, Yale University School of Medicine, New Haven, CT 06510 USA) , Lane, Maura A. (Department of Genetics, Yale University School of Medicine, New Haven, CT 06510 USA) , Mis, Emily (Department of Genetics, Yale University School of Medicine, New Haven, CT 06510 USA) , Khokha, Mustafa K. (Department of Genetics, Yale University School of Medicine, New Haven, CT 06510 USA) , Doudna, Jennifer A. (Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 USA) , Giraldez, Antonio J. (Department of Genetics, Yale University School of Medicine, New Haven, CT 06510 USA)
AI-Helper
Cpf1 is a novel class of CRISPR-Cas DNA endonucleases, with a wide range of activity across different eukaryotic systems. Yet, the underlying determinants of this variability are poorly understood. Here, we demonstrate that LbCpf1, but not AsCpf1, ribonucleoprotein complexes allow efficient mutagene...
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