Fucosterol exerts protection against amyloid β-induced neurotoxicity, reduces intracellular levels of amyloid β and enhances the mRNA expression of neuroglobin in amyloid β-induced SH-SY5Y cells
Gan, Sook Yee
(Department of Life Science, School of Pharmacy, International Medical University, 126 Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur, Malaysia)
,
Wong, Li Zhe
(School of Postgraduate Studies, International Medical University, Jalan Jalil Perkasa 19, 57000 Kuala Lumpur, Malaysia)
,
Wong, Jia Wun
(BPharm, School of Pharmacy, International Medical University, 126 Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur, Malaysia)
,
Tan, Eng Lai
(Department of Life Science, School of Pharmacy, International Medical University, 126 Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur, Malaysia)
Abstract Alzheimer's disease (AD) is a neurodegenerative disease that leads to progressive loss of neurons which often results in deterioration of memory and cognitive function. The development of AD is highly associated with the formation of senile plaques and neurofibrillary tangles. Amyloid &bet...
Abstract Alzheimer's disease (AD) is a neurodegenerative disease that leads to progressive loss of neurons which often results in deterioration of memory and cognitive function. The development of AD is highly associated with the formation of senile plaques and neurofibrillary tangles. Amyloid β (Aβ) induces neurotoxicity and contributes to the development of AD. Recent evidences also highlighted the importance of neuroglobin (Ngb) in ameliorating AD. This study assessed the ability of fucosterol, a phytosterol found in brown alga, in protecting SH-SY5Y cells against Aβ-induced neurotoxicity. Its effects on the mRNA levels of APP and Ngb as well as the intracellular Aβ levels were also determined in Aβ-induced SH-SY5Y cells. SH-SY5Y cells were exposed to fucosterol prior to Aβ treatment. The effect on apoptosis was determined using Annexin V FITC staining and mRNA expression was studied using RT-PCR. Flow cytometry confirmed the protective effects of fucosterol on SH-SY5Y cells against Aβ-induced apoptosis. Pretreatment with fucosterol increased the Ngb mRNA levels but reduced the levels of APP mRNA and intracellular Aβ in Aβ-induced SH-SY5Y cells. These observations demonstrated the protective properties of fucosterol against Aβ-induced neurotoxicity in neuronal cells. Highlights Aβ plays a role in AD, leading to several lesion events and eventually neuronal loss. Reduced brain levels of neuroglobin correlated with an increased risk of AD. Fucosterol exerts protective effects on SH-SY5Y cells against Aβ-induced apoptosis. Fucosterol enhances the mRNA expression of neuroglobin in Aβ-induced SH-SY5Y cells. Fucosterol down-regulates the mRNA expression of APP and reduces intracellular Aβ levels in Aβ-induced SH-SY5Y cells. Graphical abstract [DISPLAY OMISSION]
Abstract Alzheimer's disease (AD) is a neurodegenerative disease that leads to progressive loss of neurons which often results in deterioration of memory and cognitive function. The development of AD is highly associated with the formation of senile plaques and neurofibrillary tangles. Amyloid β (Aβ) induces neurotoxicity and contributes to the development of AD. Recent evidences also highlighted the importance of neuroglobin (Ngb) in ameliorating AD. This study assessed the ability of fucosterol, a phytosterol found in brown alga, in protecting SH-SY5Y cells against Aβ-induced neurotoxicity. Its effects on the mRNA levels of APP and Ngb as well as the intracellular Aβ levels were also determined in Aβ-induced SH-SY5Y cells. SH-SY5Y cells were exposed to fucosterol prior to Aβ treatment. The effect on apoptosis was determined using Annexin V FITC staining and mRNA expression was studied using RT-PCR. Flow cytometry confirmed the protective effects of fucosterol on SH-SY5Y cells against Aβ-induced apoptosis. Pretreatment with fucosterol increased the Ngb mRNA levels but reduced the levels of APP mRNA and intracellular Aβ in Aβ-induced SH-SY5Y cells. These observations demonstrated the protective properties of fucosterol against Aβ-induced neurotoxicity in neuronal cells. Highlights Aβ plays a role in AD, leading to several lesion events and eventually neuronal loss. Reduced brain levels of neuroglobin correlated with an increased risk of AD. Fucosterol exerts protective effects on SH-SY5Y cells against Aβ-induced apoptosis. Fucosterol enhances the mRNA expression of neuroglobin in Aβ-induced SH-SY5Y cells. Fucosterol down-regulates the mRNA expression of APP and reduces intracellular Aβ levels in Aβ-induced SH-SY5Y cells. Graphical abstract [DISPLAY OMISSION]
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