[논문]Actin stabilizer TAGLN2 potentiates adoptive T cell therapy by boosting the inside-out costimulation via lymphocyte function-associated antigen-1

Jeon, Bu-Nam; Kim, Hye-Ran; Chung, Yun Shin; Na, Bo-Ra; Park, Hyunkyung; Hong, Chorong; Fatima, Yasmin; Oh, Hyeonju; Kim, Chang-Hyun; Jun, Chang-Duk

doi:10.1080/2162402X.2018.1500674

[해외논문] Actin stabilizer TAGLN2 potentiates adoptive T cell therapy by boosting the inside-out costimulation via lymphocyte function-associated antigen-1 원문보기

Oncoimmunology, v.7 no.12, 2018년, pp.e1500674 -

Jeon, Bu-Nam (School of Life Sciences, GIST , Gwangju, Korea) , Kim, Hye-Ran (School of Life Sciences, GIST , Gwangju, Korea) , Chung, Yun Shin (School of Life Sciences, GIST , Gwangju, Korea) , Na, Bo-Ra (School of Life Sciences, GIST , Gwangju, Korea) , Park, Hyunkyung (School of Life Sciences, GIST , Gwangju, Korea) , Hong, Chorong (School of Life Sciences, GIST , Gwangju, Korea) , Fatima, Yasmin (School of Life Sciences, GIST , Gwangju, Korea) , Oh, Hyeonju (School of Life Sciences, GIST , Gwangju, Korea) , Kim, Chang-Hyun (School of Life Sciences, GIST , Gwangju, Korea) , Jun, Chang-Duk (School of Life Sciences, GIST , Gwangju, Korea)

Abstract ▼ AI-Helper

ABSTRACTCorrect temporal and spatial control of actin dynamics is essential for the cytotoxic T cell effector function against tumor cells. However, little is known whether actin engineering in tumor-targeted T cells can enhance their antitumor responses, thereby potentiating the adoptive T cell therapy. Here, we report that TAGLN2, a 22-KDa actin-stabilizing protein which is physically associated with lymphocyte function-associated antigen-1 (LFA-1), potentiates the OTI TCR CD8+ T cells to kill the intercellular adhesion molecule-1 (ICAM-1)-positive/OVA-presenting E0771 cells, but not ICAM-1-negative OVA-B16F10 cells, suggesting an ‘inside-out’ activation of LFA-1, which causes more efficient immunological synapse formation between T cells and tumor cells. Notably, recombinant TAGLN2 fused with the protein transduction domain (TG2P) overcame the disadvantages of viral gene delivery, leading to a significant reduction in tumor growth in mice. TG2P also potentiated the CD19-targeted, chimeric antigen receptor (CAR)-modified T cells to kill Raji B-lymphoma cells. Our findings indicate that activating the TAGLN2–actin–LFA-1 axis is an effective strategy to potentiate the adoptive T-cell immunotherapy.

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참고문헌 (44)

1. Intlekofer AM , Thompson CB. At the bench: preclinical rationale for CTLA-4 and PD-1 blockade as cancer immunotherapy . J Leukoc Biol . 2013 ; 94 ( 1 ): 25 – 39 . doi: 10.1189/jlb.1212621 . 23625198

상세보기
2. Sharma P , Allison JP The future of immune checkpoint therapy . Science . 2015 ; 348 ( 6230 ): 56 – 61 . doi: 10.1126/science.aaa8172 . 25838373

상세보기
3. Yao S , Zhu Y , Chen L Advances in targeting cell surface signalling molecules for immune modulation . Nat Rev Drug Discovery . 2013 ; 12 ( 2 ): 130 – 146 . doi: 10.1038/nrd3877 . 23370250

상세보기
4. June CH , Maus MV , Plesa G , Johnson LA , Zhao Y , Levine BL , Grupp SA , Porter DL Engineered T cells for cancer therapy . Cancer Immunol, ImmunoTher . 2014 ; 63 ( 9 ): 969 – 975 . doi: 10.1007/s00262-014-1568-1 . 24943274

상세보기
5. Rabinovich GA , Gabrilovich D , Sotomayor EM Immunosuppressive strategies that are mediated by tumor cells . Annu Rev Immunol . 2007 ; 25 ( 1 ): 267 – 296 . doi: 10.1146/annurev.immunol.25.022106.141609 . 17134371

상세보기
6. Kershaw MH , Westwood JA , Slaney CY , Darcy PK Clinical application of genetically modified T cells in cancer therapy . Clin Transl Immunol . 2014 ; 3 ( 5 ): e16 . doi: 10.1038/cti.2014.7 .

상세보기
7. Svane IM , Verdegaal EM Achievements and challenges of adoptive T cell therapy with tumor-infiltrating or blood-derived lymphocytes for metastatic melanoma: what is needed to achieve standard of care? Cancer Immunol, ImmunoTher . 2014 ; 63 ( 10 ): 1081 – 1091 . doi: 10.1007/s00262-014-1580-5 . 25099366

상세보기
8. Grakoui A The immunological synapse: a molecular machine controlling T cell activation . Science . 1999 ; 285 ( 5425 ): 221 – 227 . doi: 10.1126/science.285.5425.221 . 10398592

상세보기
9. Davis DM , Chiu I , Fassett M , Cohen GB , Mandelboim O , Strominger JL The human natural killer cell immune synapse . Proc Natl Acad Sci USA . 1999 ; 96 ( 26 ): 15062 – 15067 . doi: 10.1073/pnas.96.26.15062 . 10611338

상세보기
10. Meuer SC , Hussey RE , Fabbi M , Fox D , Acuto O , Fitzgerald KA , Hodgdon JC , Protentis JP , Schlossman SF , Reinherz EL An alternative pathway of T-cell activation: a functional role for the 50 kd T11 sheep erythrocyte receptor protein . Cell . 1984 ; 36 ( 4 ): 897 – 906 . doi: 10.1016/0092-8674(84)90039-4 . 6231105

상세보기
11. Boise LH , Minn AJ , Noel PJ , June CH , Accavitti MA , Lindsten T , Thompson CB CD28 costimulation can promote T cell survival by enhancing the expression of Bcl-xL . Immunity . 1995 ; 3 ( 1 ): 87 – 98 . doi: 10.1016/1074-7613(95)90161-2 . 7621080

상세보기
12. Radvanyi LG , Shi Y , Vaziri H , Sharma A , Dhala R , Mills GB , Miller RG CD28 costimulation inhibits TCR-induced apoptosis during a primary T cell response . J Immunol . 1996 ; 156 ( 5 ): 1788 – 1798 . 8596028

상세보기
13. Samstag Y , Eibert SM , Klemke M , Wabnitz GH Actin cytoskeletal dynamics in T lymphocyte activation and migration . J Leukoc Biol . 2003 ; 73 ( 1 ): 30 – 48 . doi: 10.1189/jlb.0602272.http . 12525560

상세보기
14. van Kooyk Y , van Vliet SJ , Figdor CGC The actin cytoskeleton regulates LFA-1 ligand binding through avidity rather than affinity changes . J Biol Chem . 1999 ; 274 ( 38 ): 26869 – 26877 . doi: 10.1074/jbc.274.38.26869 . 10480895

상세보기
15. Lees-Miller JP , Heeley DH , Smillie LB , Kay CM Isolation and characterization of an abundant and novel 22-kDa protein (SM22) from chicken gizzard smooth muscle . J Biol Chem . 1987 ; 262 ( 7 ): 2988 – 2993 . 3818630
16. Shapland C , Hsuan JJ , Totty NF , Lawson D Purification and properties of transgelin: a transformation and shape change sensitive actin-gelling protein . J Cell Biol . 1993 ; 121 ( 5 ): 1065 – 1073 . doi: 10.1083/jcb.121.5.1065 . 8501116

상세보기
17. Na B-R , Kim H-R , Piragyte I , Oh H-M , Kwon M-S , Akber U , Lee H-S , Park D-S , Song WK , Park Z-Y , et al TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse . J Cell Biol . 2015 ; 209 ( 1 ): 143 – 162 . doi: 10.1083/jcb.201407130 . 25869671

상세보기
18. Katagiri K , Hattori M , Minato N , Kinashi T Rap1 functions as a key regulator of T-cell and antigen-presenting cell interactions and modulates T-cell responses . Mol Cell Biol . 2002 ; 22 ( 4 ): 1001 – 1015 . doi: 10.1128/MCB.22.4.1001 . 11809793

상세보기
19. Katagiri K , Maeda A , Shimonaka M , Kinashi T RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1 . Nat Immunol . 2003 ; 4 ( 8 ): 741 – 748 . doi: 10.1038/ni950 . 12845325

상세보기
20. Sebzda E , Bracke M , Tugal T , Hogg N , Cantrell DA Rap1A positively regulates T cells via integrin activation rather than inhibiting lymphocyte signaling . Nat Immunol . 2002 ; 3 ( 3 ): 251 – 258 . doi: 10.1038/ni765 . 11836528

상세보기
21. Kamimura K , Suda T , Zhang G , Liu D Advances in gene delivery systems . Pharmaceut Med . 2011 ; 25 ( 5 ): 293 – 306 . doi: 10.1007/BF03256872 . 22200988

상세보기
22. van den Berg A , Dowdy SF Protein transduction domain delivery of therapeutic macromolecules . Curr Opin Biotechnol . 2011 ; 22 ( 6 ): 888 – 893 . doi: 10.1016/j.copbio.2011.03.008 . 21489777

상세보기
23. Pandit S , Zhou Y , Shiue L , Coutinho-Mansfield G , Li H , Qiu J , Huang J , Yeo GW , Ares M , Fu XD Genome-wide analysis reveals SR protein cooperation and competition in regulated splicing . Mol Cell . 2013 ; 50 ( 2 ): 223 – 235 . doi: 10.1016/j.molcel.2013.03.001 . 23562324

상세보기
24. Piragyte I , Jun C-D Actin engine in immunological synapse . Immune Netw . 2012 ; 12 ( 3 ): 71 – 83 . doi: 10.4110/in.2012.12.3.71 . 22916042

원문보기 상세보기
25. Ritter AT , Angus KL , Griffiths GM The role of the cytoskeleton at the immunological synapse . Immunol Rev . 2013 ; 256 ( 1 ): 107 – 117 . doi: 10.1111/imr.12117 . 24117816

상세보기
26. Kim H-R , Lee H-S , Lee K-S , Jung ID , Kwon M-S , Kim C-H , Kim S-M , Yoon M-H , Park Y-M , Lee S-M , et al An essential role for TAGLN2 in phagocytosis of lipopolysaccharide-activated macrophages . Sci Rep . 2017 ; 7 ( 1 ): 8731 . doi: 10.1038/s41598-017-09144-x . 28821818

상세보기
27. Wang C , Morley SC , Donermeyer D , Peng I , Lee WP , Devoss J , Danilenko DM , Lin Z , Zhang J , Zhou J , et al Actin-bundling protein L-plastin regulates T cell activation . J Immunol . 2010 ; 185 ( 12 ): 7487 – 7497 . doi: 10.4049/jimmunol.1001424 . 21076065

상세보기
28. Eibert SM , Lee K-H , Pipkorn R , Sester U , Wabnitz GH , Giese T , Meuer SC , Samstag Y Cofilin peptide homologs interfere with immunological synapse formation and T cell activation . Proc Natl Acad Sci USA . 2004 ; 101 ( 7 ): 1957 – 1962 . doi: 10.1073/pnas.0308282100 . 14762171

상세보기
29. Gimona M , Mital R The single CH domain of calponin is neither sufficient nor necessary for F-actin binding . J Cell Sci . 1998 ; 111 ( 13 ): 1813 – 1821 . 9625744

상세보기
30. Comrie WA , Burkhardt JK Action and traction: cytoskeletal control of receptor triggering at the immunological synapse . Front Immunol . 2016 ; 7 : (MAR):1–25 . doi: 10.3389/fimmu.2016.00068 .

상세보기
31. Reina M , Espel E Role of LFA-1 and ICAM-1 in cancer . Cancers . 2017 ; 9 ( 12 ): 1 – 14 . doi: 10.3390/cancers9110153 .

상세보기
32. Mukai S , Kagamu H , Shu S , Plautz GE Critical role of CD11a (LFA-1) in therapeutic efficacy of systemically transferred antitumor effector T cells . Cell Immunol . 1999 ; 192 : 122 – 132 . doi: 10.1006/cimm.1998.1439 . 10087180

상세보기
33. Dustin ML , Springer TA T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1 . Nature . 1989 ; 341 ( 6243 ): 619 – 624 . doi: 10.1038/341619a0 . 2477710

상세보기
34. Kandula S , Abraham C LFA-1 on CD4+ T cells is required for optimal antigen-dependent activation in vivo . J Immunol . 2004 ; 173 ( 7 ): 4443 – 4451 . doi: 10.4049/jimmunol.173.7.4443 . 15383575

상세보기
35. Park EJ , Peixoto A , Imai Y , Goodarzi A , Cheng G , Carman CV , Von Andrian UH , Shimaoka M Distinct roles for LFA-1 affinity regulation during T-cell adhesion, diapedesis, and interstitial migration in lymph nodes . Blood . 2010 ; 115 ( 8 ): 1572 – 1581 . doi: 10.1182/blood-2009-08-237917 . 20023213

상세보기
36. Dippold W , Wittig B , Schwaeble W , Mayet W , Meyer zum Buschenfelde KH Expression of intercellular adhesion molecule 1 (ICAM-1, CD54) in colonic epithelial cells . Gut . 1993 ; 34 ( 11 ): 1593 – 1597 . doi: 10.1136/gut.34.11.1593 . 7902311

상세보기
37. Wimmenauer S , Keller H , Ruckauer KD , Rahner S , Wolff-Vorbeck G , Kirste G , von Kleist S , Farthman EH Expression of CD44, ICAM-1 and N-CAM in colorectal cancer. Correlation with the tumor stage and the phenotypical characteristics of tumor- infiltrating lymphocytes . Anticancer Res . 1997 ; 17 ( 4A ): 2395 – 2400 . 9252653
38. Lu W , Dong Z , Donawho C , Fidler IJ ICAM-1 expression and the soluble ICAM-1 level for evaluating the metastatic potential of gastric cancer . Int J Cancer . 2002 ; 100 ( 4 ): 486 – 490 . doi: 10.1002/ijc.10514 . 12115535

상세보기
39. Tachimori A , Yamada N , Sakate Y , Yashiro M , Maeda K , Ohira M , Nishino H , Hirakawa K Up regulation of ICAM-1 gene expression inhibits tumour growth and liver metastasis in colorectal carcinoma . Eur J Cancer . 2005 ; 41 ( 12 ): 1802 – 1810 . doi: 10.1016/j.ejca.2005.04.036 . 16051479

상세보기
40. Basingab FS , Ahmadi M , Morgan DJ IFNγ-dependent interactions between ICAM-1 and LFA-1 counteract prostaglandin E2-mediated inhibition of antitumor CTL responses . Cancer Immunol Res . 2016 ; 4 ( 5 ): 400 – 411 . doi: 10.1158/2326-6066.CIR-15-0146 . 26928462

상세보기
41. Schröder C , Witzel I , Müller V , Krenkel S , Wirtz RM , Jänicke F , Schumacher U , Milde-Langosch K Prognostic value of intercellular adhesion molecule (ICAM)-1 expression in breast cancer . J Cancer Res Clin Oncol . 2011 ; 137 ( 8 ): 1193 – 1201 . doi: 10.1007/s00432-011-0984-2 . 21590495

상세보기
42. Dezfouli S , Hatzinisiriou I , Ralph SJ Enhancing CTL responses to melanoma cell vaccines in vivo: synergistic increases obtained using IFNγ primed and IFNβ treated B7-1 + B16-F10 melanoma cells . Immunol Cell Biol . 2003 ; 81 ( 6 ): 459 – 471 . doi: 10.1046/j.0818-9641.2003.01189.x . 14636243

상세보기
43. Harada H , Kizaka-Kondoh S , Hiraoka M Antitumor protein therapy; application of the protein transduction domain to the development of a protein drug for cancer treatment . Breast Cancer . 2006 ; 13 ( 1 ): 16 – 26 . doi: 10.2325/jbcs.13.16 . 16518058

상세보기
44. Eggermont LJ , Paulis LE , Tel J , Figdor CG Towards efficient cancer immunotherapy: advances in developing artificial antigen-presenting cells . Trends Biotechnol . 2014 ; 32 ( 9 ): 456 – 465 . doi: 10.1016/j.tibtech.2014.06.007 . 24998519

상세보기

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[해외논문] Actin stabilizer TAGLN2 potentiates adoptive T cell therapy by boosting the inside-out costimulation via lymphocyte function-associated antigen-1 원문보기

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[해외논문] Actin stabilizer TAGLN2 potentiates adoptive T cell therapy by boosting the inside-out costimulation via lymphocyte function-associated antigen-1 원문보기

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