UV irradiation on a skin brings about the qualitative and quantitative alterations of the extracellular matrix. Repeated-UV irradiation suppressed the synthesis of collagen and activated the expression of the matrix metalloprotease (MMP). In this paper, on the purpose of development of novel anti-ag...
UV irradiation on a skin brings about the qualitative and quantitative alterations of the extracellular matrix. Repeated-UV irradiation suppressed the synthesis of collagen and activated the expression of the matrix metalloprotease (MMP). In this paper, on the purpose of development of novel anti-aging agents from natural sources, effects of several natural products on in vitro MMP-1 activity and UVA induced MMP-1 synthesis in human dermal fibroblast (HDF) culture were studied. We measured MMP-1 activities by fluorescence assay using gelatin as substrates. As a result, the extract of Dicentra spectabilis, and flower buds of Tussilago farfara showed strong inhibitory effect. Among them, the extract of flower buds of Tussilago fartara and Dicentra spectabilis inhibited MMP-1 activity by 92% and 87% at 0.05% (w/v). And UVA induced MMP-1 expression were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in HDF culture. The extract of flower buds of Tussilago farfara and Dicentra spectabilis suppressed the UVA induced expression of MMP-1 by similar level of Vitamin C 200$\mu$M at 0.1% (w/v). These results suggest that the extract of Dicentra spectabilis, and flower buds of Tussilago farfara effectively prevent skin from the UV-induced photoaging. So the extracts are thought to have potential as effective raw materials for anti-aging cosmetics.
UV irradiation on a skin brings about the qualitative and quantitative alterations of the extracellular matrix. Repeated-UV irradiation suppressed the synthesis of collagen and activated the expression of the matrix metalloprotease (MMP). In this paper, on the purpose of development of novel anti-aging agents from natural sources, effects of several natural products on in vitro MMP-1 activity and UVA induced MMP-1 synthesis in human dermal fibroblast (HDF) culture were studied. We measured MMP-1 activities by fluorescence assay using gelatin as substrates. As a result, the extract of Dicentra spectabilis, and flower buds of Tussilago farfara showed strong inhibitory effect. Among them, the extract of flower buds of Tussilago fartara and Dicentra spectabilis inhibited MMP-1 activity by 92% and 87% at 0.05% (w/v). And UVA induced MMP-1 expression were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in HDF culture. The extract of flower buds of Tussilago farfara and Dicentra spectabilis suppressed the UVA induced expression of MMP-1 by similar level of Vitamin C 200$\mu$M at 0.1% (w/v). These results suggest that the extract of Dicentra spectabilis, and flower buds of Tussilago farfara effectively prevent skin from the UV-induced photoaging. So the extracts are thought to have potential as effective raw materials for anti-aging cosmetics.
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대상 데이터
In this paper, for an attempt to develop materials protecting UV-induced skin damage, 20 plant extracts were evaluated for their inhibitory activities on MMPs.
이론/모형
Fig 4. The effects of several plant extracts on the expression of MMP-1 evaluated by ELISA method.
To evaluate effects of plant extracts on the MMP production from UVA irradiated HDFs, Enzyme-linked immunosorbent assay (ELISA) were used. To determine whether the plant extracts could modulate the production of MMP-1 on UVA irradiated HDFs, each plant extracts was applied for 24hr after UVA irradiation to the cells, In the presence of ethanol extract of flower buds of Tussilago farfara and ethanol extract of Dicentra spectabilis, the UVA induced expression of MMP-1 was suppressed about 92% and 80% at a concentration of 0.
성능/효과
And the result of the gelatin-based zymography in HDF culture showed that in the presence of the ethanol extract of flower buds of Tussilago farfara and ethanol extract of Dicentra spectabilis showed significantly decreased MMP-1 and MMP-2 activity in comparison to UVA irradiated control. Enzyme-linked immunosorbent assay (ELISA) were used to evaluate the MMP production from UVA irradiated HDFs.
And, MMP-1 inhibition activity assay by fluorescence method revealed the extract of Dicentra spectabilis, and flower buds of Tussilago farfara had strong inhibitory effect by 92% and 87% at a concentration of 0.02%(w/v).
From the results of these screening procedures, we suggest that the extract of Dicentra spectabilis, and flower buds of Tussilago farfara, which can suppress the expression of MMPs and inhibit the activities of MMPs, can protect the skin from photoaging.
To determine whether the plant extracts could modulate the production of MMP-1 on UVA irradiated HDFs, each plant extracts was applied for 24hr after UVA irradiation to the cells. In the presence of ethanol extract of flower buds of Tussilago farfara and ethanol extract of Dicentra spectabilis showed significantly decreased MMP-1 and MMP-2 activity at 0.1% (w/v) (Lane 3, 6) in comparison to UVA irradiated control.
These results suggest that the extract of Dicentra spectabilis, and flower buds of Tussilago farfara have the strong inhibitory activity on MMP-1 activity, and suppressive effect of the UVA induced MMP-1 synthesis.
immunosorbent assay (ELISA) were used. To determine whether the plant extracts could modulate the production of MMP-1 on UVA irradiated HDFs, each plant extracts was applied for 24hr after UVA irradiation to the cells, In the presence of ethanol extract of flower buds of Tussilago farfara and ethanol extract of Dicentra spectabilis, the UVA induced expression of MMP-1 was suppressed about 92% and 80% at a concentration of 0.1% (w/v).
후속연구
05%(w/v). From these results, the extract of Dicentra spectabilis, and flower buds of Tussilago farfara were selected for further study.
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