초록 ▼

There is provided a brazzein variant comprising XXKCKXXYXNYPXXKCXXAXQCNYDCKLDKHARXGECFYDEXRNX XCXCDYCXYX (SEQ ID NO: 1), wherein X, X, and X are each independently an amino acid with polar uncharged side chain, X is an amino acid with a negatively charged side chain, X is a glutamine (E) or an amino

There is provided a brazzein variant comprising XXKCKXXYXNYPXXKCXXAXQCNYDCKLDKHARXGECFYDEXRNX XCXCDYCXYX (SEQ ID NO: 1), wherein X, X, and X are each independently an amino acid with polar uncharged side chain, X is an amino acid with a negatively charged side chain, X is a glutamine (E) or an amino acid with positively charged side chains, X, X, X, X, and X are each independently an amino acid with hydrophobic side chain, X is a lysine (K) or an amino acid with polar uncharged side chain, X is an arginine (R) or an amino acid with polar uncharged side chain, X is an arginine (R) or an amino acid with negatively charged side chain, X is an amino acid with positively charged side chain, X is an alanine or an amino acid with polar uncharged side chain, X is an amino acid with polar uncharged side chain, X is a glutamic acid, a glutamine, or a lysine, and X is a proline, an arginine, a threonine, or no residue. Also disclosed are polynucleotides encoding the brazzein variant, a cell expressing the brazzein variant, and a method of producing the brazzein variant.

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