초록

- 감염형 병원균의 고속 고감도 분자진단을 위한 나노포어센서 플랫폼 개발
- 질병진단을 위한 바이오마커 선정 및 해당 수용체의 고정화 기술 확립
- 고감도의 전기적 감지기구 제작
- Multiplex 검출을 위한 나노포어 감지소자 어레이 개발

Abstract ▼

There has been a rapid advances in biosensor technology. Glucose sensor, the first commercially successful biosensor, has become an essential tool for the people with diabetes. In addition, there are many other clinically useful biosensors developed by modem technologies. Specificity is the most pow

There has been a rapid advances in biosensor technology. Glucose sensor, the first commercially successful biosensor, has become an essential tool for the people with diabetes. In addition, there are many other clinically useful biosensors developed by modem technologies. Specificity is the most powerful aspect of biosensors. Many biosensors utilize the specific recognition ability of biological ligands or receptors. On the other hand, the specific recognition event should be converted to a colorimetric or electrical signal that we can perceive through appropriate devices. An accurate diagnosis for a specific disease or infection requires certain level of marker molecules in clinical specimen. But by the time that the amount of biomarker reaches to the level that we can detect, the progression of disease or infection often advances to the stage that is hard for early treatment and preventing spreads of infectious agents to other animals. In early stage of infection, the amount of infectious agent is often very low. Thus, it is critical to develop a method to detect extreamly low amount of target molecules, biomarkers. The goal of this research is to develop a platform technology for the detection of low level of infectious agents in farm animal in early stage and thus enable us for effective treatment and prevention of further spreading of the infectious agents to other animals. We developed a sensor platform technology that can detect target molecules in highly sensitive manner. For this, we employed nanoporous materials and electrical readout devices which will potentially be applicable to multiplex detection setup. The measurement is based on the current changes upon target binding on nanoporous membrane. The simple setup in combination with functional nanomaterials is suitable for the development of portable equipment. It will find wide applications in point of care diagnostic system and contribute to ensure the safety and health of farm animal.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 7
• CONTENTS ... 8
• 목차 ... 10
• 제 1 장 서론 ... 12
• 1. 연구개발의 목적 및 필요성 ... 12
• 제 2 장 국내외 기술개발 현황 ... 13
• 1. 국내기술개발 현황 ... 13
• 2. 국외기술개발 현황 ... 14
• 3. 국내.외 연구현황 ... 15
• 제 3 장 연구개발수행 내용 ... 16
• 1. 연구개발의 최종목표 및 성격 ... 16
• 2. 연차별 연구내용 ... 16
• 3. 연차별 연구개발의 추진일정 ... 17
• 4. 정량적 성과목표 ... 18
• 5. 평가의 착안점 및 기준 ... 19
• 제 4 장 연구개발의 결과 ... 21
• 1. 1차년도 결과 ... 21
• 2. 2차년도 결과 ... 22
• 3. 3차년도 결과 ... 23
• 4. 최종 결과 ... 28
• 제 5 장 연구개발 목표 달성도 및 기대성과 ... 31
• 1. 연구목표 ... 31
• 2. 국내성과 ... 33
• 3. 국외성과 ... 34
• 제 6 장 연구개발결과의 활용계획 ... 35
• 1. 연구결과의 발전계획 ... 35
• 2. 연구결과의 기대성과 ... 35
• 제 7 장 연구개발과정에서 수집한 해외과학기술정보 ... 37
• 제 8 장 기타 중요 변동사항 ... 39
• 제 9 장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 40
• 제 10 장 참고문헌 ... 41
• 끝페이지 ... 44